2019-12-11RELPDBePDBePDBe2018-06-142018-08-222019-01-162019-12-11Medical Research Council (United Kingdom)MC_UU_12014/7United KingdomBiotechnology and Biological Sciences Research CouncilBB/J013854/1United KingdomFeline Calicivirus Strain F9Conley MJBhella DConley MJMcElwee MAzmi LGabrielsen MByron OGoodfellow IGBhella DCalicivirus VP2 forms a portal-like assembly following receptor engagement.NatureUK565377381201930626974doi:10.1038/s41586-018-0852-11476-46870006NATUASEMD-0054associated EM volumeFeline Calicivirus Strain F96gshFULLOVERLAPT=3 Icosahedral Capsid.T=3 Icosahedral Capsid.01T=3 Icosahedral CapsidFeline calicivirusFelis catusVP1Feline calicivirus0.0733466643Felis catusLEVOMCSTCANVLKYYNWDPHFRLVIDPNKFLSVGFCDNPLMCCYPELLPEFGTVWDCDQSPLQIYLESILGDDEWASTYEAID
PAVPPMHWDAAGKIFQPHPGVLMHHLIGEVAKAWDPNLPMFRLEADGDGSITAPEQGTPVGGVIAEPSAQMSAAADMATG
KSVDSEWEAFFSFHTSVNWSTSETQGKILFKQSLGPLLNPYLEHLAKLYVAWSGSIDVRFSISGSGVFGGKLAAIVVPPG
VDPVQSTSMLQYPHVLFDARQVEPVIFSIPDLRSTLYHLMSDTDTTSLVIMVYNDLINPYANDSNSSGCIVTVETKPGAD
FKFHLLKPPGSMLTHGSVPSDLIPKSSSLWIGNRHWTDITDFVIRPFVFQANRHFDFNQETAGWSTPRYRPITITISEKN
GAKLGIGVATDYIVPGIPDGWPDTTIPEKLTPAGDYAITNKSGNDITTAAGYDGADVIVNNTNFKGMYICGSLQRAWGDK
KISNTAFITTATKVDNAIEPSNVIDMTKIAVYQDTHVGKEVQTSDDTLSLLGYTGIGEQAIGSDRDRVVRISVLPETGAR
GGNHPIFYKNSIKLGYVIRSIDVFNSQILHTSRQLSLNHYLLPPDSFAVYRIIDSNGSWFDIGIDSDGFSFVGVSSIGKL
EFPLTASYMGIQLAKIRLASNIRSSMTKLPOTASSIUM ION3.9098e-053KsingleParticleparticle17.2Phosphate buffered salineETHANE100277FEI VITROBOT MARK IVPurified enveloped virionsFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.775000.0FEI FALCON III (4k x 4k)INTEGRATING1519863.0Each micrograph was recorded as a movie of 50 individual fractions with a total dose of 63 e/angstrom squared1Images were motion-corrected using motioncor2
Defocus estimation was performed using GCTF59531Autopicking in RelionGctfCTF correction was implemented through RelionPreviously published models calculated at lower resolution.I3.0FSC 0.143 CUT-OFFRELION2.141436PROJECTION MATCHINGRELION2.1MAXIMUM LIKELIHOODRELION2.110RELION2.1Origin and orientation values were determined by 3D auto refine in RELION, assuming full icosahedral symmetry (i.e. with full gold-standard methods).