2020-11-25RELPDBePDBePDBe2018-07-302018-08-222019-07-312020-11-25Medical Research Council (United Kingdom)MC_U105663141United KingdomRhesus macaque mitochondrial complex IAgip ANABlaza JNFedor JGHirst JAgip AABlaza JNFedor JGHirst JMammalian Respiratory Complex I Through the Lens of Cryo-EM.Annu Rev Biophys48165184201930786232doi:10.1146/annurev-biophys-052118-1157041936-1238EMD-0151associated EM volumeRhesus macaque mitochondrial complex IRhesus macaque mitochondrial complex I0Macaca mulattaHeartMitochondria1singleParticleparticle37.14150.0NaClSodium chloride20.0Tris0.05DDMQuantifoil, UltrAuFoilGOLD300GLOW DISCHARGEUltrAuFoil gold grids (0.6/1) was used for the preparation of macaque complex I grids. In brief, UltrAuFoil gold grids (0.6/1) were glow discharged at 20 mA for 60 sec using a EMITECH glow-discharger unit. Then, the grids were incubated in 5 mM 11-mercaptoundecyl hexaethyleneglycol (SPT-0011P6, SensoPath Technologies) in ethanol for two days under anaerobic conditions.ETHANE100277FEI VITROBOT MARK IV8-16s blotting.. The sample was collected from the peak fraction of a size-exclusion column and was not concentrated.FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.759000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENFEI FALCON II (4k x 4k)INTEGRATING2.050.01Gctf1.06C1FOURIER SPACE4.1FSC 0.143 CUT-OFFRELION2.1patch b141688PROJECTION MATCHINGRELION2.1patch b1PROJECTION MATCHINGRELION2.1patch b14RELION2.1patch b1emd_0151_msk_1.mapemd_0151_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
360
360360
0
00360360360500.4500.4500.490.090.090.0XYZ-0.034489490.149893-0.00000357060.00433739231.391.391.39emd_0151_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)