2019-12-11RELPDBePDBePDBe2018-10-222019-07-242019-07-242019-12-11Structure of McrBC without DNA binding domains (one half of the full complex)Itoh YNirwan NSaikrishnan KAmunts ANirwan NItoh YSingh PBandyopadhyay SVinothkumar KRAmunts ASaikrishnan KStructure-based mechanism for activation of the AAA+ GTPase McrB by the endonuclease McrC.Nat CommunUK1030583058201931296862doi:10.1038/s41467-019-11084-12041-1723EMD-0310associated EM volumeStructure of McrBC without DNA binding domains (one half of the full complex)6hz4FULLOVERLAPMcrB and McrC complex without DNA binding domainsMcrB and McrC complex without DNA binding domains012The N-terminal DNA binding domain of McrB is truncatedEscherichia coli K-12Escherichia coliBL21-AI0.515-methylcytosine-specific restriction enzyme BEscherichia coli (strain K12)K120.0357584926Escherichia coliLEVOMSKTESYCLEDALNDLFIPETTIETILKRLTIKKNIILQGPPGVGKTFVARRLAYLLTGEKAPQRVNMVQFHQSYSYEDF
IQGYRPNGVGFRRKDGIFYNFCQQAKEQPEKKYIFIIDEINRANLSKVFGEVMMLMEHDKRGENWSVPLTYSENDEERFY
VPENVYIIGLMNTADRSLAVVDYALRRRFSFIDIEPGFDTPQFRNFLLNKKAEPSFVESLCQKMNELNQEISKEATILGK
GFRIGHSYFCCGLEDGTSPDTQWLNEIVMTDIAPLLEEYFFDDPYKQQKWTNKLLGDSSGSHHHHHH3.1.21.-Protein McrCEscherichia coli (strain K12)K120.0406436252Escherichia coliLEVOMEQPVIPVRNIYYMLTYAWGYLQEIKQANLEAIPGNNLLDILGYVLNKGVLQLSRRGLELDYNPNTEIIPGIKGRIEFAK
TIRGFHLNHGKTVSTFDMLNEDTLANRIIKSTLAILIKHEKLNSTIRDEARSLYRKLPGISTLHLTPQHFSYLNGGKNTR
YYKFVISVCKFIVNNSIPGQNKGHYRFYDFERNEKEMSLLYQKFLYEFCRRELTSANTTRSYLKWDASSISDQSLNLLPR
METDITIRSSEKILIVDAKYYKSIFSRRMGTEKFHSQNLYQLMNYLWSLKPENGENIGGLLIYPHVDTAVKHRYKINGFD
IGLCTVNLGQEWPCIHQELLDIFDEYLKPHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER0.0005221963GNPMAGNESIUM ION2.4305e-053MGGUANOSINE-5'-DIPHOSPHATE0.0004432013GDPsingleParticleparticle4.07.40.1NaClsodium chloride10.0Tris-HCltris(hydroxymethyl)aminomethane hydrochloride buffer5.0MgCl2Magnesium chloride2.0C10H17N6O13P35'-Guanylyl imidodiphosphateC-flat-2/2 4CCOPPER400GLOW DISCHARGEAIRETHANE100277FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30070.02.70.35.0130000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGIF Quantum LS20GATAN K2 SUMMIT (4k x 4k)COUNTING2-20133268.01.51771763Gctf1.06C13.6FSC 0.143 CUT-OFFRELION2.1.0225201ANGULAR RECONSTITUTIONRELION2.1.0ANGULAR RECONSTITUTIONRELION2.1.0RELION2.1.0AB INITIO MODELCross-correlation coefficientREAL74emd_0310_msk_1.mapemd_0310_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
240
240240
0
00240240240251.99998251.99998251.9999890.090.090.0XYZ-0.0116872620.0509790780.00036685710.0031849181.051.051.05emd_0310_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)