2023-10-11RELRCSBRCSBRCSB2018-11-202019-02-062019-02-062023-10-11National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)R35 GM122588United StatesMicroED structure of Proteinase K at 2.75A resolution from a single milled crystal.Martynowycz MWZhao Wbroad-spectrum serum proteinase, HYDROLASEMartynowycz MWZhao WHattne JJensen GJGonen TCollection of Continuous Rotation MicroED Data from Ion Beam-Milled Crystals of Any Size.StructureUK27545548.e2201930661853doi:10.1016/j.str.2018.12.0031878-41862005STRUE66n4uFULLOVERLAPProteinase KProteinase K01Engyodontium album0.02893Proteinase KEngyodontium album0.0289587911Engyodontium albumLEVOAAQTNAPWGLARISSTSPGTSTYYYDESAGQGSCVYVIDTGIEASHPEFEGRAQMVKTYYYSSRDGNGHGTHCAGTVGSR
TYGVAKKTQLFGVKVLDDNGSGQYSTIIAGMDFVASDKNNRNCPKGVVASLSLGGGYSSSVNSAAARLQSSGVMVAVAAG
NNNADARNYSPASEPSVCTVGASDRYDRRSSFSNYGSVLDIFGPGTDILSTWIGGSTRSISGTSMATPHVAGLAAYLMTL
GKTTAASACRYIADTANKGDLSNIPFGTVNLLAYNNYQAP068733.4.21.64CALCIUM ION4.0078e-052CASULFATE ION9.606299999999999e-051SO4water1.8015e-0523HOHelectronCrystallographythreeDArray207.5Quantifoil R2/2COPPER200CARBONHOLEY12.0GLOW DISCHARGEOTHERETHANE100273.0FEI VITROBOT MARK IVProteinase K purchased from Sigma.FEI TALOS ARCTICAFLOOD BEAMDIFFRACTIONFIELD EMISSION GUN200100.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGEN77.090.0FEI CETA (4k x 4k)4096409611002.00.04Continuous rotation from -30 to +30 with a rotation rate of 0.2 degrees per second and a readout every 3 seconds.17001This was the new CetaD.2.75DIFFRACTION PATTERN/LAYERLINESPHENIX2.8.2310131080788.4000000000000060.390.4722.4122.4102.752.753.1483183128.10999999999999988.2300000000000042.95I9SPDBexperimental modelRIGID BODY FITRRECIPROCAL21.100000000000001