2019-02-20
REL
RCSB
RCSB
RCSB
2018-11-20
2019-01-16
2019-02-06
2019-02-20
Apo form metabotropic glutamate receptor 5 with Nanobody 43
Koehl A
Hu H
Feng D
Zhang Y
Sun B
Kobilka TS
Pardon E
Steyaert J
Mathiesen JM
Skiniotis G
Kobilka BK
Koehl A
Hu H
Feng D
Sun B
Zhang Y
Robertson MJ
Chu M
Kobilka TS
Laermans T
Steyaert J
Tarrasch J
Dutta S
Fonseca R
Weis WI
Mathiesen JM
Skiniotis G
Kobilka BK
Structural insights into the activation of metabotropic glutamate receptors.
Nature
UK
566
79
84
2019
30675062
doi:10.1038/s41586-019-0881-4
1476-4687
0006
NATUAS
EMD-0347
associated EM volume
EMD-0346
other EM volume
EMD-0345
other EM volume
Metabotropic Glutamate Receptor 5 - Nb43 Complex
Metabotropic Glutamate Receptor 5 - Nb43 Complex
0
1
2
Complex between metabotropic glutamate receptor 5 and Nb43
Homo sapiens
Spodoptera frugiperda
SF9
0.22
Metabotropic Glutamate Receptor 5
Homo sapiens
Polypeptide encoding metabotropic glutamate receptor 5
Spodoptera frugiperda
LEVO
QSSERRVVAHMPGDIIIGALFSVHHQPTVDKVHERKCGAV
REQYGIQRVEAMLHTLERINSDPTLLPNITLGCEIRDSCWHSAVALEQSIEFIRDSLISS
EEEEGLVRCVDGSSSSFRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLS
DKTLFKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGESGMEAFKDMSAKEGI
CIAHSYKIYSNAGEQSFDKLLKKLTSHLPKARVVACFCEGMTVRGLLMAMRRLGLAGEFL
LLGSDGWADRYDVTDGYQREAVGGITIKLQSPDVKWFDDYYLKLRPETNHRNPWFQEFWQ
HRFQCRLEGFPQENSKYNKTCNSSLTLKTHHVQDSKMGFVINAIYSMAYGLHNMQMSLCP
GYAGLCDAMKPIDGRKLLESLMKTNFTGVSGDTILFDENGDSPGRYEIMNFKEMGKDYFD
YINVGSWDNGELKMDDDEVWSKKSNIIRSVCSEPCEKGQIKVIRKGEVSCCWTCTPCKEN
EYVFDEYTCKACQLGSWPTDDLTGCDLIPVQYLRWGDPEPIAAVVFACLGLLATLFVTVV
FIIYRDTPVVKSSSRELCYIILAGICLGYLCTFCLIAKPKQIYCYLQRIGIGLSPAMSYS
ALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFILICIQLGIIVALFIMEPPDIM
HDYPSIREVYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYT
TCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTS
TVVRMHVGDGKSSSAASRSSSLVNL
Nanobody 43
Lama glama
Nanobody 43
Escherichia coli
LEVO
QVQLVESGGGLVQAGGSLRLSCAASGRTFTSYAMGWFRQAPGKERESVAAISSSGGSTHYADSVKGRFTISRDNSKNTVYLQMNSLKPEDTAVYYCAAAMYGSRWPDWEYDYWGQGTQVTVSSAA
singleParticle
particle
10
7.5
100.0
NaCl
sodium chloride
20.0
HEPES
HEPES
0.02
GDN
Glyco Disogenin
0.002
CHS
Cholesteryl Hemisuccinate
Quantifoil R1.2/1.3
GOLD
200
GLOW DISCHARGE
ETHANE
Sample was mono disperse as assayed by gel filtration and SDS PAGE.
FEI TITAN KRIOS
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
50.0
29000.
58139.
GATAN K2 SUMMIT (4k x 4k)
COUNTING
50.0
1
The apo structure was used as starting model.
7.9
FSC 0.143 CUT-OFF
RELION
2.1
44831
RANDOM ASSIGNMENT
MAXIMUM LIKELIHOOD