2023-09-06RELRCSBRCSBRCSB2019-01-122019-02-202019-07-102023-09-06Howard Hughes Medical Institute (HHMI)United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)P01GM051487United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)F32GM122334United StatesLife Sciences Research FoundationUnited StatesCryo-EM structure of the T. thermophilus Csm complex bound to target ssRNALiu JJLiu TYCsm, RNA substrate, RNA BINDING PROTEINLiu TYLiu J-JAditham AJNogales EDoudna JATarget preference of Type III-A CRISPR-Cas complexes at the transcription bubbleNat CommunUK2019doi:10.1038/s41467-019-10780-22041-17236o1ounknownThermus thermophilus Csm complex bound to target ssRNAThermus thermophilus Csm complex bound to target ssRNA01Thermus thermophilusRNAThermus thermophilusGGAUAUGCGCCUUGACGUGGUCGUCCCCGGGCGCCUUAUCUACGGUAUCAsingleParticleparticle7.9ETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300DIRECT ELECTRON DE-16 (4k x 4k)45.013.8FSC 0.143 CUT-OFF87321ANGULAR RECONSTITUTIONMAXIMUM LIKELIHOOD