2019-04-17RELRCSBRCSBRCSB2019-01-312019-02-272019-03-132019-04-17Big conformation of apo CRISPR_Csm complexZhang KPintilie GLi SZhu YChiu WHuang ZGuo MZhang KZhu YPintilie GDGuan XLi SSchmid MFMa ZChiu WHuang ZCoupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry.Cell Res.CN29305312201930814678doi:10.1038/s41422-019-0151-x1748-7838EMD-0518associated EM volumeBig conformation of apo CRISPR_Csm complexEMD-0516other EM volumeSmall conformation of ssRNA-bound CRISPR_Csm complexEMD-0517other EM volumeBig conformation of ssRNA-bound CRISPR_Csm complexEMD-0519other EM volumeSmall conformation of apo CRISPR_Csm complexBig conformation of apo CRISPR_Csm complexBig conformation of apo CRISPR_Csm complex0Streptococcus thermophilusEscherichia coli 'BL21-Gold(DE3)pLysS AG'singleParticleparticle0.47.6unspecifiedETHANE100FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)7.013.4FSC 0.143 CUT-OFFcryoSPARC0.6584024MAXIMUM LIKELIHOODMAXIMUM LIKELIHOOD