2024-03-20RELRCSBRCSBRCSB2019-01-312019-02-272019-03-132024-03-20National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)P41GM103832United StatesNational Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)U54GM103297United StatesNational Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)R01GM079429United StatesNational Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)S10OD021600United StatesNational Natural Science Foundation of China (NSFC)31825008ChinaNational Natural Science Foundation of China (NSFC)31422014ChinaSmall conformation of apo CRISPR_Csm complexZhang KPintilie GCRISPR, Type III-A, ssRNAase, ssDNase, HYDROLASE, TRANSFERASE-RNA complexGuo MZhang KZhu YPintilie GDGuan XLi SSchmid MFMa ZChiu WHuang ZCoupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry.Cell Res.CN29305312201930814678doi:10.1038/s41422-019-0151-x1748-7838EMD-0516other EM volumeSmall conformation of ssRNA-bound CRISPR_Csm complexEMD-0517other EM volumeBig conformation of ssRNA-bound CRISPR_Csm complexEMD-0518other EM volumeBig conformation of apo CRISPR_Csm complex6nueFULLOVERLAPSmall conformation of apo CRISPR_Csm complexSmall conformation of apo CRISPR_Csm complex012345Streptococcus thermophilusCRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)Streptococcus thermophilus0.0869306721Escherichia coli 'BL21-Gold(DE3)pLysS AG'LEVOMKKEKIDLFYGALLHDIGKVIQRATGERKKHALVGADWFDEIADNQVISDQIRYHMANYQSDKLGNDHLAYITYIADNIA
SGVDRRQSNEESDEDASAKIWDTYTNQADIFNVFGAQTDKRYFKPTVLNLKSKPNFASATYEPFSKGDYAAIATRIKNEL
AEFEFNQAQIDSLLNLFEAILSFVPSSTNSKEIADISLAEHSRLTAAFALAIYDYLEDKGRHNYKEDLFTKASAFYEEEA
FLLASFDLSGIQDFIYNIATSGAAKQLKARSLYLDFMSEYIADSLLDKLGLNRANLLYVGGGHAYFVLANTEKTVETLVQ
FEKDFNQFLLANFQTRLYVAFGWGSFAAKDIMSELNSPESYRQIYQKASRMISEKKISRYDYRTLMLLNRGGKSSERECE
ICHSVENLVSYHDQKVCDICRGLYQFSKEIAHDHFIITENEGLPIGPNACLKGVAFEKLSQESFSRVYVKNDYKAGTIKA
THVFVGDYQCDEIHKYAALSKNEDGLGIKRLAVVRLDVDDLGAAFMAGFSRQGNGQYSTLSRSATFSRSMSLFFKVYINQ
FASDKKLSIIYAGGDDVFAIGSWQDIIAFTVELRQNFIKWTNGKLTLSAGIGLFADKTPISLMAHQTGELEEAAKGNEKD
SISLFSSDYTFKFDRFITNVYDDKLEQIRYFFNHQDERGKNFIYKLIELLRNYESEEKMNVARLAYYLTRLEELTDKDER
DKFKQFKKLFFKWYTNNESDRKEAELALLLYVYEIRKDA0A0A7HFE13.1.-.-CRISPR system Cms protein Csm2Streptococcus thermophilus0.0142383913Escherichia coli 'BL21-Gold(DE3)pLysS AG'LEVOMAILTDENYVDKAERAISLLEKDNKGNYLLTTSQIRKLLSLCSSLYDRSKERKFDELINDVSYLRVQFVYQAGREIAVKD
LIEKAQILEALKEIKDRETLQRFCRYMEALVAYFKFYGGKDA0A0A7HIX1CRISPR type III-associated RAMP protein Csm3Streptococcus thermophilus0.0246009185Escherichia coli 'BL21-Gold(DE3)pLysS AG'LEVOMTFAKIKFSAQIRLETGLHIGGSDAFAAIGAIDSPVIKDPITNIPIIPGSSLKGKMRTLLAKVYNEKVAEKPSDDSDILS
RLFGNSKDKRFKMGRLIFRDAFLSNADELDSLGVRSYTEVKFENTIDRITAEANPRQIERAIRNSTFDFELIYEITDENE
NQVEEDFKVIRDGLKLLELDYLGGSGSRGYGKVAFEKLKATTVFGNYDVKTLNELLTAEVA0A0A7HIF0crRNAStreptococcus thermophilus0.0229355529999999971ACGGAAACUUUCGUAACUGUUUAAUUCUGUUCACUUAUUCCACCGAUAUAAACCUAAUUACCUCGAGAGGGGCRISPR type III-associated RAMP protein Csm4Streptococcus thermophilus0.0337869491Escherichia coli 'BL21-Gold(DE3)pLysS AG'LEVOMTYKLYIMTFQNAHFGSGTLDSSKLTFSADRIFSALVLESLKMGKLDAFLAEANQDKFTLTDAFPFQFGPFLPKPIGYPK
HDQIDQSVDVKEVRRQAKLSKKLQFLALENVDDYLNGELFENEEHAVIDTVTKNQPHKDGNLYQVATTRFSNDTSLYVIA
NESDLLNELMSSLQYSGLGGKRSSGFGRFELDIQNIPLELSDRLTKNHSDKVMSLTTALPVDADLEEAMEDGHYLLTKSS
GFAFSHATNENYRKQDLYKFASGSTFSKTFEGQIVDVRPLDFPHAVLNYAKPLFFKLEVA0A0A7HGA1ADENOSINE-5'-TRIPHOSPHATE0.0005071811ATPsingleParticleparticle0.47.6unspecifiedETHANE100FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)7.013.3FSC 0.143 CUT-OFFcryoSPARC0.6581540MAXIMUM LIKELIHOODMAXIMUM LIKELIHOOD