2019-12-04RELRCSBRCSBRCSB2019-02-132019-02-202019-11-062019-12-04National Institutes of Health/National Institute of General Medical Sciences2R01GM111730-05United StatesCryo-EM Map of the active Ragulator-RagA-RagC ComplexYokom ALFromm SAHurley JHLawrence REFromm SAFu YYokom ALKim DJThelen AMYoung LNLim CYSamelson AJHurley JHZoncu RStructural mechanism of a Rag GTPase activation checkpoint by the lysosomal folliculin complex.ScienceUS366971977201931672913doi:10.1126/science.aax03641095-92030038SCIEASEMD-0554other EM volumeEMD-7072other EM volumeEMD-0556associated EM volumeactive RagA-RagC-Ragulator Complexactive RagA-RagC-Ragulator Complex0123456789RagA bound to GTP; RagC bound to XDP
RagC has T90N and D181N mutationsHomo sapiensSpodoptera frugiperdaSf90.15singleParticleparticle0.57.425.0HEPES130.0NaClsodium chloride2.5MgCl2magnesium chloride0.5TCEPC-flat-2/1COPPER400CARBONHOLEYETHANE90298FEI VITROBOT MARK IVFEI TITAN KRIOSOTHERBRIGHT FIELDFIELD EMISSION GUN3002.7FEI TITAN KRIOS AUTOGRID HOLDERNITROGEN20GATAN K2 SUMMIT (4k x 4k)SUPER-RESOLUTION8.048.01GctfcryoSPARC v2 ab-initioC18.1FSC 0.143 CUT-OFFcryoSPARC275996NOT APPLICABLENOT APPLICABLE