2024-03-27
REL
PDBj
PDBj
PDBj
2019-01-15
2019-05-29
2019-05-29
2024-03-27
Ministry of Science and Technology (China)
2016YFA0501100 to H.W.
China
The reconstruction of apo-state streptavidin at 3.3 Angstrom resolution
Fan X
Wang J
Lei JL
Wang HW
streptavidin, CYTOSOLIC PROTEIN
Fan X
Wang J
Zhang X
Yang Z
Zhang JC
Zhao L
Peng HL
Lei J
Wang HW
Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution.
Nat Commun
UK
10
2386
2386
2019
31160591
doi:10.1038/s41467-019-10368-w
2041-1723
EMD-0689
other EM volume
the same protein with biotin
6j6k
FULLOVERLAP
Streptavidin
Streptavidin
0
1
Streptomyces avidinii
0.052
Streptavidin
Streptomyces avidinii
0.012596640999999999
4
LEVO
GITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQ
YVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVK
P22629
singleParticle
particle
0.2
7.5
25.0
(HOCH2)3CNH2
Tris-HCl
75.0
NaCl
Sodium chloride
ETHANE
100
285
FEI VITROBOT MARK IV
FEI TITAN KRIOS
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
50.0
0.01
-0.8
-0.8
215000.0
FEI TITAN KRIOS AUTOGRID HOLDER
NITROGEN
VOLTA PHASE PLATE
spherical aberration corrector
GATAN K2 SUMMIT (4k x 4k)
SUPER-RESOLUTION
3838
3710
1-32
1
1450
2.56
50.0
1
709967
Relion 3D initial model reconstruction
1
D2
3.3
FSC 0.143 CUT-OFF
RELION
23991
RANDOM ASSIGNMENT
RELION
MAXIMUM LIKELIHOOD
RELION
3
26430.0
RELION