2022-03-30RELPDBePDBePDBe2019-06-052019-06-122020-02-052022-03-30National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM082251United StatesThe Francis Crick InstituteFC001061United KingdomSIVrcm intasomeCherepanov PNans ACook NCook NJLi WBerta DBadaoui MBallandras-Colas ANans AKotecha ARosta EEngelman ANCherepanov PStructural basis of second-generation HIV integrase inhibitor action and viral resistance.ScienceUS367806810202032001525doi:10.1126/science.aay49191095-92030038SCIEASEMD-10041associated EM volumeSIVrcm intasome6rwlFULLOVERLAPSIVrcm intasome in complex with bictegravirSIVrcm intasome in complex with bictegravir012345Pol Protein1123Simian immunodeficiency virusEscherichia coliDNA145Simian immunodeficiency virussynthetic constructPol proteinSimian immunodeficiency virus0.0327321828Escherichia coliLEVOGFLDGIEKAQEEHEKYHNNWRAMAEDFQIPQVVAKEIVAQCPKCQVKGEAMHGQVDASPKTWQMDCTHLEGKVIIVAVHV
ASGYIEAEVLPAETGKETAHFLLKLAARWPVKHLHTDNGDNFTSSAVQAVCWWAQIEHTFGVPYNPQSQGVVESMNHQLK
TIITQIRDQAEKIETAVQMAVLIHNFKRKGGIGGYSAGERIIDIIASDLQTTKLQNQISKIQNFRVYFREGRDQQWKGPA
TLIWKGEGAVVIQDGQDLKVVPRRKCKIIKDYGRKDVDSETSMEGRQEKDPol proteinSimian immunodeficiency virus0.0326751312Escherichia coliLEVOFLDGIEKAQEEHEKYHNNWRAMAEDFQIPQVVAKEIVAQCPKCQVKGEAMHGQVDASPKTWQMDCTHLEGKVIIVAVHVA
SGYIEAEVLPAETGKETAHFLLKLAARWPVKHLHTDNGDNFTSSAVQAVCWWAQIEHTFGVPYNPQSQGVVESMNHQLKT
IITQIRDQAEKIETAVQMAVLIHNFKRKGGIGGYSAGERIIDIIASDLQTTKLQNQISKIQNFRVYFREGRDQQWKGPAT
LIWKGEGAVVIQDGQDLKVVPRRKCKIIKDYGRKDVDSETSMEGRQEKDPol proteinSimian immunodeficiency virus0.0326881699999999962Escherichia coliLEVOGFLDGIEKAQEEHEKYHNNWRAMAEDFQIPQVVAKEIVAQCPKCQVKGEAMHGQVDASPKTWQMDCTHLEGKVIIVAVHV
ASGYIEAEVLPAETGKETAHFLLKLAARWPVKHLHTDNGANFTSSAVQAVCWWAQIEHTFGVPYNPQSQGVVESMNHQLK
TIITQIRDQAEKIETAVQMAVLIHNFKRKGGIGGYSAGERIIDIIASDLQTTKLQNQISKIQNFRVYFREGRDQQWKGPA
TLIWKGEGAVVIQDGQDLKVVPRRKCKIIKDYGRKDVDSETSMEGRQEKDDNA (5'-D(P*GP*CP*TP*AP*AP*GP*AP*AP*AP*AP*AP*TP*CP*TP*CP*TP*AP*CP*CP*A)-3')Simian immunodeficiency virus0.0092239952(DG)(DT)(DT)(DC)(DT)(DA)(DG)(DA)(DA)(DG)(DG)(DC)(DT)(DA)(DA)(DG)(DA)(DA)(DA)(DA)
(DA)(DT)(DC)(DT)(DC)(DT)(DA)(DC)(DC)(DA)DNADNA (5'-D(*AP*AP*CP*TP*GP*GP*TP*AP*GP*AP*GP*AP*TP*TP*TP*TP*TP*CP*TP*TP*AP*GP*C)-3')Simian immunodeficiency virus0.0101345409999999992(DA)(DA)(DC)(DT)(DG)(DG)(DT)(DA)(DG)(DA)(DG)(DA)(DT)(DT)(DT)(DT)(DT)(DC)(DT)(DT)
(DA)(DG)(DC)(DC)(DT)(DT)(DC)(DT)(DA)(DG)(DA)(DA)(DC)DNAZINC ION6.5409e-058ZNsingleParticleparticle7.00.35NaClsodium chloride0.025Hepes4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid0.005MgSO4magnesium sulfateETHANE95295FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.71.63.5FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)COUNTING1-302802750.41Gctf1.06per-particle estimationab initio, as implemented in CryoSPARCC23.36FSC 0.143 CUT-OFFcryoSPARC2171893RANDOM ASSIGNMENTcryoSPARCMAXIMUM LIKELIHOODcryoSPARC18RELION2.12B4J1EX41K6YRIGID BODY FITREALemd_10041_msk_1.mapemd_10041_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
360
360360
0
00360360360496.8496.8496.890.090.090.0XYZ-0.462784742.22798630.00333574650.0598699821.381.381.38Unsharpened CryoSPARC mapemd_10041_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
360
360360
0
00360360360496.8496.8496.890.090.090.0XYZ-1.5880453.95533750.0033357120.0772417041.381.381.38Autosharpened map from CryoSPARCemd_10041_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
360
360360
0
00360360360496.8496.8496.890.090.090.0XYZ-0.52900452.34878970.00305872180.082270531.381.381.38Half map Bemd_10041_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
360
360360
0
00360360360496.8496.8496.890.090.090.0XYZ-0.56686972.35099320.00304289140.082360551.381.381.38Half map A