2019-12-11
REL
PDBe
PDBe
PDBe
2019-10-02
2019-11-20
2019-12-04
2019-12-11
German Research Foundation
SPP1879-WI3285/5-2
Germany
Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis
Muller C
Beckert B
Wilson DN
Shimokawa-Chiba N
Muller C
Fujiwara K
Beckert B
Ito K
Wilson DN
Chiba S
Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis.
Nat Commun
UK
10
5397
5397
2019
31776341
doi:10.1038/s41467-019-13408-7
2041-1723
EMD-10353
associated EM volume
BrfA, a RF-dependent ribosome rescue factor in the Gram-positive bacterium Bacillus subtilis
6szs
FULLOVERLAP
E. coli 70S stalled on truncated mRNA in complex BsBrfA and BsRF2-Q251P
E. coli 70S stalled on truncated mRNA in complex BsBrfA and BsRF2-Q251P
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E. coli 70S stalled on truncated mRNA in complex with Bacillus ribosome rescue factor A (BrfA) and a hydrolysis deminished mutant of Bacillus peptide chain release factor 2 (RF2-Q251P)
Escherichia coli ribosome with P-site tRNA-Pro(CGG, carrying the nascent chain
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Escherichia coli K-12
Escherichia coli K-12
Bacillus subtilis ribosome rescue factor (BrfA; former YqkK) and peptide chain release factor 2 (RF2)
1
55
56
Bacillus subtilis (strain 168)
Escherichia coli BL21(DE3)
50S ribosomal protein L32
Escherichia coli (strain K12)
0.006463445
1
Escherichia coli K-12
LEVO
MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK
50S ribosomal protein L33
Escherichia coli (strain K12)
0.006388631
1
Escherichia coli K-12
LEVO
MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVRQHVIYKEAKIK
50S ribosomal protein L34
Escherichia coli (strain K12)
0.005397463
1
Escherichia coli K-12
LEVO
MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK
50S ribosomal protein L35
Escherichia coli (strain K12)
0.007313032
1
Escherichia coli K-12
LEVO
MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHLRPKAMVSKGDLGLVIACLPYA
50S ribosomal protein L36
Escherichia coli (strain K12)
0.00437739
1
Escherichia coli K-12
LEVO
MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG
50S ribosomal protein L31
Escherichia coli (strain K12)
0.007887117
1
Escherichia coli K-12
LEVO
MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFFTGKQRDVATGGRVDRFNKRFNIPGSK
23S ribosomal RNA
Escherichia coli K-12
0.941306188
1
GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGUCAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCG
GUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAUGGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGA
AUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAUCUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCC
AGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAUCAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGA
UACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGUGAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUG
AAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAA
GAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUGUACGUACAAGCAGUGGGAGCACGCUUAGGCGUGUGACUGC
GUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUC
UUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCCGGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACU
AACUGGAGGACCGAACCGACUAAUGUUGAAAAAUUAGCGGAUGACUUGUGGCUGGGGGUGAAAGGCCAAUCAAACCGGGA
GAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGUGAAUUCAUCUCCGGGGGUAGAGCACUGUUUCGGCAAGG
GGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUACCGGAGAAUGUUAUCACGGGAGACACACGGCGGGUGCUAA
CGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUAAGGUCCCAAAGUCAUGGUUAAGUGGGAAACGAUGUGGGAA
GGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGGCCUG
CGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUGCGGCAGCGACGCUUAUGCGUUGUUGGGUAGGGGAGCGUUC
UGUAAGCCUGCGAAGGUGUGCUGUGAGGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACAUAAGUAACGAUAAAGCGG
GUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCCAACGUUAAUCGGGGCAGGGUGAGUCGACCCCUAAGGCGAG
GCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGGUGUUACUGCGAAGGGGGGACGGAGAAGGCUAU
GUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGCUGGUUUUCCAGGCAAAUCCGGAAAAUCAAGGCUGAGGCGU
GAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUGCUUCCAGGAAAAGCCUCUAAGCAUCAGGUAACAUCAAAUC
GUACCCCAAACCGACACAGGUGGUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCGGGUGAAGGAACUAGGCAAAAU
GGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGUGAGGUCCCUCGCGGAUGGAGCUGAAAUCAGUCGAAGAUAC
CAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGCAAACACGAAAGUGGACGUAUACGGUGUGACGCCUGCCCGG
UGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCUCUUGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAAC
GGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACC
CGAGACUCAGUGAAAUUGAACUCGCUGUGAAGAUGCAGUGUACCCGCGGCAAGACGGAAAGACCCCGUGAACCUUUACUA
UAGCUUGACACUGAACAUUGAGCCUUGAUGUGUAGGAUAGGUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGC
CGACCUUGAAAUACCACCCUUUAAUGUUUGAUGUUCUAACGUUGACCCGUAAUCCGGGUUGCGGACAGUGUCUGGUGGGU
AGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGGAGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGU
UAGUGCAAUGGCAUAAGCCAGCUUGACUGCGAGCGUGACGGCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGG
UUCUGAAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUC
GACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGAAGUAGGUCCCAAGGGUAUGGCUGUUCGCCA
UUUAAAGUGGUACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCCCUAUCUGCCGUGGGCGCUGGAGAACUGAG
GGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAUCACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUGCCCGGU
AGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGCACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACCCUUUAA
GGGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGCCGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAACCGGUAC
UAAUGAACCGUGAGGCUUAACCU
5S ribosomal RNA
Escherichia coli (strain K12)
0.03879009
1
UGCCUGGCGGCCGUAGCGCGGUGGUCCCACCUGACCCCAUGCCGAACUCAGAAGUGAAACGCCGUAGCGCCGAUGGUAGU
GUGGGGUCUCCCCAUGCGAGAGUAGGGAACUGCCAGGCAU
50S ribosomal protein L2
Escherichia coli (strain K12)
0.029923619
1
Escherichia coli K-12
LEVO
MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVER
LEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNF
GKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK
50S ribosomal protein L3
Escherichia coli (strain K12)
0.022277535
1
Escherichia coli K-12
LEVO
MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDGYRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLW
EFRLAEGEEFTVGQSISVELFADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA
50S ribosomal protein L4
Escherichia coli (strain K12)
0.022121566
1
Escherichia coli K-12
LEVO
MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRS
GGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA
50S ribosomal protein L5
Escherichia coli (strain K12)
0.020333611
1
Escherichia coli K-12
LEVO
MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIR
QGYPIGCKVTLRGERMWEFFERLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
KSDEEGRALLAAFDFPFRK
50S ribosomal protein L6
Escherichia coli (strain K12)
0.018932791
1
Escherichia coli K-12
LEVO
MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVT
EGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
GVRYADEVVRTKEAKKK
50S ribosomal protein L9
Escherichia coli (strain K12)
0.01578902
1
Escherichia coli K-12
LEVO
MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEARRAELEAKLAEVLAAANARAEKINALETVTI
ASKAGDEGKLFGSIGTRDIADAVTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE
50S ribosomal protein L13
Escherichia coli (strain K12)
0.016050606
1
Escherichia coli K-12
LEVO
MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGH
IGGIKQATFEEMIARRPERVIEIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI
50S ribosomal protein L14
Escherichia coli (strain K12)
0.013565067
1
Escherichia coli K-12
LEVO
MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFD
GNACVLLNNNSEQPIGTRIFGPVTRELRSEKFMKIISLAPEVL
50S ribosomal protein L15
Escherichia coli (strain K12)
0.015008471
1
Escherichia coli K-12
LEVO
MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLS
DLAKVEGGVVDLNTLKAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE
50S ribosomal protein L16
Escherichia coli (strain K12)
0.015312269
1
Escherichia coli K-12
LEVO
MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAV
RMGKGKGNVEYWVALIQPGKVLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM
50S ribosomal protein L17
Escherichia coli (strain K12)
0.014393657
1
Escherichia coli K-12
LEVO
MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLF
NELGPRFASRAGGYTRILKCGFRAGDNAPMAYIELVDRSEKAEAAAE
50S ribosomal protein L18
Escherichia coli (strain K12)
0.012794668
1
Escherichia coli K-12
LEVO
MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAE
RALEKGIKDVSFDRSGFQYHGRVQALADAAREAGLQF
50S ribosomal protein L19
Escherichia coli (strain K12)
0.013159278
1
Escherichia coli K-12
LEVO
MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPV
VDSISVKRRGAVRKAKLYYLRERTGKAARIKERLN
50S ribosomal protein L20
Escherichia coli (strain K12)
0.013528024
1
Escherichia coli K-12
LEVO
MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFI
NGLKKASVEIDRKILADIAVFDKVAFTALVEKAKAALA
50S ribosomal protein L21
Escherichia coli (strain K12)
0.011586374
1
Escherichia coli K-12
LEVO
MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRR
KHYRKQQGHRQWFTDVKITGISA
50S ribosomal protein L22
Escherichia coli (strain K12)
0.012253359
1
Escherichia coli K-12
LEVO
METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGP
SMKRIMPRAKGRADRILKRTSHITVVVSDR
50S ribosomal protein L23
Escherichia coli (strain K12)
0.01122216
1
Escherichia coli K-12
LEVO
MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDW
KKAYVTLKEGQNLDFVGGAE
50S ribosomal protein L24
Escherichia coli (strain K12)
0.01133925
1
Escherichia coli K-12
LEVO
MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKA
DRVGFRFEDGKKVRFFKSNSETIK
50S ribosomal protein L25
Escherichia coli (strain K12)
0.010713465
1
Escherichia coli K-12
LEVO
MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDHDKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRH
PYKPKLQHIDFVRA
50S ribosomal protein L27
Escherichia coli (strain K12)
0.00914654
1
Escherichia coli K-12
LEVO
MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFI
SIEAE
50S ribosomal protein L28
Escherichia coli (strain K12)
0.009027551
1
Escherichia coli K-12
LEVO
MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEKRFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY
50S ribosomal protein L29
Escherichia coli (strain K12)
0.007286464
1
Escherichia coli K-12
LEVO
MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAGA
50S ribosomal protein L30
Escherichia coli (strain K12)
0.00655482
1
Escherichia coli K-12
LEVO
MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAVSFMVKVEE
16S ribosomal RNA
Escherichia coli (strain K12)
0.499054625
1
AAAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGA
AGCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAAUGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGA
AACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUG
GGAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGA
ACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGC
CGCGUGUAUGAAGAAGGCCUUCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUU
GACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAGCAGCCGCGGUAAUACGGAGGGUGCAAGCGUUAAUCGGAA
UUACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAGAUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGA
UACUGGCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACC
GGUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCAAACAGGAUUAGAUACCCUGG
UAGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCCUUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACC
GCCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAU
UCGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAGAAUGUGCCUUCGGGAACCGU
GAGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGAAAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCC
UUUGUUGCCAGCGGUCCGGCCGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUC
AUCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCGACCUCGCGAGAGCAAGCGGA
CCUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACUCGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCA
GAAUGCCACGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGU
AGCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGAAGUCGUAACAAGGUAACCGUAGGGGAAC
CUGCGGUUGGAUCACCUCCU
30S ribosomal protein S2
Escherichia coli (strain K12)
0.02678167
1
Escherichia coli K-12
LEVO
MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELNKIASRKGKILFVGTKRAASEAV
KDAALSCDQFFVNHRWLGGMLTNWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVAATVREGRSQDLASQAEESFVEA
E
30S ribosomal protein S3
Escherichia coli (strain K12)
0.026031316
1
Escherichia coli K-12
LEVO
MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLTKELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKK
GEDVEKLRKVVADIAGVPAQINIAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPEKPAAQPKKQQRKGRK
30S ribosomal protein S4
Escherichia coli (strain K12)
0.023514199
1
Escherichia coli K-12
LEVO
MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAA
RLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK
30S ribosomal protein S5
Escherichia coli (strain K12)
0.017629398
1
Escherichia coli K-12
LEVO
MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNGRVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGT
LQHPVKGVHTGSRVFMQPASEGTGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
VEEILGK
30S ribosomal protein S6
Escherichia coli (strain K12)
0.015727512
1
Escherichia coli K-12
LEVO
MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRF
NDAVIRSMVMRTKHAVTEASPMVKAKDERRERRDDFANETADDAEAGDSEEEEEE
30S ribosomal protein S7
Escherichia coli (strain K12)
0.020055156
1
Escherichia coli K-12
LEVO
MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELEAFEVALENVRPTVEVKSRRV
GGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRWLSLR
SFSHQAGASSKQPALGYLN
30S ribosomal protein S8
Escherichia coli (strain K12)
0.014146557
1
Escherichia coli K-12
LEVO
MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEGFIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSR
PGLRIYKRKDELPKVMAGLGIAVVSTSKGVMTDRAARQAGLGGEIICYVA
30S ribosomal protein S9
Escherichia coli (strain K12)
0.01488627
1
Escherichia coli K-12
LEVO
MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETARMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIR
HGITRALMEYDESLRSELRKAGFVTRDARQVERKKVGLRKARRRPQFSKR
30S ribosomal protein S10
Escherichia coli (strain K12)
0.011755597
1
Escherichia coli K-12
LEVO
MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPT
EKTVDALMRLDLAAGVDVQISLG
30S ribosomal protein S11
Escherichia coli (strain K12)
0.013870975
1
Escherichia coli K-12
LEVO
MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIK
NLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHNGCRPPKKRRV
30S ribosomal protein S12
Escherichia coli (strain K12)
0.013768157
1
Escherichia coli K-12
LEVO
MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVI
LIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA
30S ribosomal protein S13
Escherichia coli (strain K12)
0.013128467
1
Escherichia coli K-12
LEVO
MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVKISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRL
MDLGCYRGLRHRRGLPVRGQRTKTNARTRKGPRKPIKK
30S ribosomal protein S14
Escherichia coli (strain K12)
0.01160656
1
Escherichia coli K-12
LEVO
MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNAVLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLS
RIKVREAAMRGEIPGLKKASW
30S ribosomal protein S15
Escherichia coli (strain K12)
0.010290816
1
Escherichia coli K-12
LEVO
MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQ
LIERLGLRR
30S ribosomal protein S16
Escherichia coli (strain K12)
0.009207572
1
Escherichia coli K-12
LEVO
MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIASEKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNK
AA
30S ribosomal protein S17
Escherichia coli (strain K12)
0.009724491
1
Escherichia coli K-12
LEVO
MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKLHVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVE
KAVL
30S ribosomal protein S18
Escherichia coli (strain K12)
0.009005472
1
Escherichia coli K-12
LEVO
MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRITGTRAKYQRQLARAIKRARYLSLLPYTDRHQ
30S ribosomal protein S19
Escherichia coli (strain K12)
0.010455355
1
Escherichia coli K-12
LEVO
MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNMIGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTY
RGHAADKKAKKK
30S ribosomal protein S20
Escherichia coli (strain K12)
0.009708464
1
Escherichia coli K-12
LEVO
MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLT
AQINKLA
30S ribosomal protein S21
Escherichia coli (strain K12)
0.008524039
1
Escherichia coli K-12
LEVO
MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTERKRAKASAVKRHAKKLARENARRTRLY
mRNA
Escherichia coli K-12
0.001866181
1
CCGCCG
P-site tRNA-Pro(CGG)
Escherichia coli (strain K12)
0.024846746
1
CGGUGAUUGGCGCAGCCUGGUAGCGCACUUCGUUCGGGACGAAGGGGUCGGAGGUUCGAAUCCUCUAUCACCGACCA
Uncharacterized protein YqkK
Bacillus subtilis (strain 168)
0.008113376
1
Escherichia coli BL21(DE3)
LEVO
MAKSQAKKKRGHRLRNGGRDVLLSRGSTPSFSTHGRMTKSKKEILNKRKHKNPYDHTAVDDKDFFVPQKAA
Peptide chain release factor 2
Bacillus subtilis (strain 168)
0.041638559
-Q251P mutation (hydrolysis defective release factor) -low resolution regions were only the backbone was traced were kept as ALA
1
Escherichia coli BL21(DE3)
LEVO
EIRAELENMASRLADFRGSLDLESKEARIAELDEQMADPEFWNDQQKAQTVINEANGLKDYVNSYKKLNESHEELQMTHD
LLKEEPDTDLQLELEKELKSLTKEFNEFELQLLLSEPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWGERRGFKVET
LDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSCEVMPEFNDEIDIDIRTEDIKVDTYR
ASGAGGPHVNTTDSAVRITHLPTNVVVTCQTERSQIKNRERAMKMLKAKLYQRRIEEQQAELDEIRGEQKEIGWGSQIRS
YVFHPYSMVKDHRTNTEMGNVQAVMDGDIDTFIDAYLRSKLS
singleParticle
particle
7.2
ETHANE
FEI TITAN KRIOS
SPOT SCAN
BRIGHT FIELD
FIELD EMISSION GUN
300
2.7
FEI FALCON III (4k x 4k)
83.0
1
5MGP
C1
3.06
FSC 0.143 CUT-OFF
RELION
3.0
154405
MAXIMUM LIKELIHOOD
MAXIMUM LIKELIHOOD
5MGP
REAL