2021-04-14RELPDBePDBePDBe2019-10-222020-10-142020-10-142021-04-14French National Research AgencyANR-17CE11-0028FranceMexA-MexB cryoEM mapGlavier MPuvanendran DSalvador DDecossas MPhan GGarnier CFrezza ECece QSchoehn GPicard MTaveau J-CDaury LBroutin ILambert OGlavier MPuvanendran DSalvador DDecossas MPhan GGarnier CFrezza ECece QSchoehn GPicard MTaveau JCDaury LBroutin ILambert OAntibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex.Nat CommunUK1149484948202033009415doi:10.1038/s41467-020-18770-52041-1723EMD-10395associated EM volumeMexAB complex in nanodisc interacting with OprM moleculesMexAB complex in nanodisc interacting with OprM molecules012Pseudomonas aeruginosaEscherichia coliMexBPseudomonas aeruginosaLEVOMSKFFIDRPIFAWVIALVIMLAGGLSILSLPVNQYPAIAPPAIAVQVSYPGASAETVQDT
VVQVIEQQMNGIDNLRYISSESNSDGSMTITVTFEQGTDPDIAQVQVQNKLQLATPLLPQ
EVQRQGIRVTKAVKNFLMVVGVVSTDGSMTKEDLSNYIVSNIQDPLSRTKGVGDFQVFGS
QYSMRIWLDPAKLNSYQLTPGDVSSAIQAQNVQISSGQLGGLPAVKGQQLNATIIGKTRL
QTAEQFENILLKVNPDGSQVRLKDVADVGLGGQDYSINAQFNGSPASGIAIKLATGANAL
DTAKAIRQTIANLEPFMPQGMKVVYPYDTTPVVSASIHEVVKTLGEAILLVFLVMYLFLQ
NFRATLIPTIAVPVVLLGTFGVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVM
AEEGLSPREAARKSMGQIQGALVGIAMVLSAVFLPMAFFGGSTGVIYRQFSITIVSAMAL
SVIVALILTPALCATMLKPIEKGDHGEHKGGFFGWFNRMFLSTTHGYERGVASILKHRAP
YLLIYVVIVAGMIWMFTRIPTAFLPDEDQGVLFAQVQTPPGSSAERTQVVVDSMREYLLE
KESSSVSSVFTVTGFNFAGRGQSSGMAFIMLKPWEERPGGENSVFELAKRAQMHFFSFKD
AMVFAFAPPSVLELGNATGFDLFLQDQAGVGHEVLLQARNKFLMLAAQNPALQRVRPNGM
SDEPQYKLEIDDEKASALGVSLADINSTVSIAWGSSYVNDFIDRGRVKRVYLQGRPDARM
NPDDLSKWYVRNDKGEMVPFNAFATGKWEYGSPKLERYNGVPAMEILGEPAPGLSSGDAM
AAVEEIVKQLPKGVGYSWTGLSYEERLSGSQAPALYALSLLVVFLCLAALYESWSIPFSV
MLVVPLGVIGALLATSMRGLSNDVFFQVGLLTTIGLSAKNAILIVEFAKELHEQGKGIVE
AAIEACRMRLRPIVMTSLAFILGVVPLAISTGAGSGSQHAIGTGVIGGMVTATVLAIFWV
PLFYVAVSTLFKDEASKQQASVEKGQMexAPseudomonas aeruginosaLEVOMQRTPAMRVLVPALLVAISALSGCGKSEAPPPAQTPEVGIVTLEAQTVTLNTELPGRTNA
FRIAEVRPQVNGIILKRLFKEGSDVKAGQQLYQIDPATYEADYQSAQANLASTQEQAQRY
KLLVADQAVSKQQYADANAAYLQSKAAVEQARINLRYTKVLSPISGRIGRSAVTEGALVT
NGQANAMATVQQLDPIYVDVTQPSTALLRLRRELASGQLERAGDNAAKVSLKLEDGSQYP
LEGRLEFSEVSVDEGTGSVTIRAVFPNPNNELLPGMFVHAQLQEGVKQKAILAPQQGVTR
DLKGQATALVVNAQNKVELRVIKADRVIGDKWLVTEGLNAGDKIITEGLQFVQPGVEVKT
VPAKNVASAQKADAAPAKTDSKGsingleParticleparticle7.4ETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 QUANTUM (4k x 4k)42.01364914Gctf3C13.2FSC 0.143 CUT-OFFAfter 3D alignment on MexB, an average map was calculed from the three aligned maps and a two-by-two comparison of the maps by FSC leads to a 3.2 angstroems resolution82432MAXIMUM LIKELIHOODRELIONMAXIMUM LIKELIHOODRELION327000.0RELIONThree 3D maps( calculed from Class3D and after REfine3D) were similar but show three different orientations (0,120,240 degree rotation) with respect to MexB