2020-10-14RELPDBePDBePDBe2020-04-062020-09-302020-09-302020-10-14Novo Nordisk FoundationNNF14CC0001DenmarkDanish Council for Independent Research8123-00002BDenmarkStructure of a protonation mimic of unplugged C. jejuni MotABSantiveri MRoa-Eguiara ATaylor NMISantiveri MRoa-Eguiara AKuhne CWadhwa NHu HBerg HCErhardt MTaylor NMIStructure and Function of Stator Units of the Bacterial Flagellar Motor.Cell183244257.e16202032931735doi:10.1016/j.cell.2020.08.0161097-4172EMD-10830associated EM volumeStructure of a protonation mimic of unplugged C. jejuni MotAB6ykrFULLOVERLAPStator unit MotAB(Delta41-60, D22N)Stator unit MotAB(Delta41-60, D22N)012The stator unit consists of a dimer of MotB surrounded by a pentamer of MotA.
Single mutation D22N simulates the protonated state of the channel.
This stator unit is unplugged (deletion of aminoacids 41 to 60 of MotB).Campylobacter jejuni subsp. jejuni 81-176Escherichia coliC43(DE3)Chemotaxis protein MotA, putativeCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)81-1760.0281958165Escherichia coliLEVOMDLSTILGMVLAVTSISVGDILEGGNPLHVIHLSSFLIVMPTAAFCAMTSTHKKIVKAAYKELKVVFKGSGVNLPERIAQ
LIEFAIIARRDGLLALESRTNEIENEFLKNAMMMLVDGKSFEEIHESMEIQTEQLEEHYKECAEYWIVFGETCPTMGLVG
AVFGLILALKLLDNPQAMAAGISGAFTATVTGIFGAYALFAPWGKKLKANGMDLVKEQIVITEAIKGIAEGANPRDLEAK
LFNFLSHDDPRISQFDKGChemotaxis protein MotB, putativeCampylobacter jejuni subsp. jejuni serotype O:23/36 (strain 81-176)81-1760.0298626432Escherichia coliLEVOMAKKHKCPECPAGEKWAVPYANFLSLLLALFIALWAISKTTQTVKEESKTQEKYKGAAKEESDELKSLKQMTMTQQETIK
RLQAALDQSDNQVALNLPSKVEFERGSAQIVSADIQDYLKRMAELTTYLPPQAKIEIRGYTDNSDSIIRSYELAYQRAEN
VLKYFIEGGANLKNISIKSYGLNNPINGNPQALENNRVEIYFKVDTADTSTQKSVLELINKIGTKAPGTLEVLFQGPGGS
GSAWSHPQFEKGGGSGGGSGGSAWSHPQFEKwater1.8015e-056HOHsingleParticleparticle0.688Quantifoil R2/1GLOW DISCHARGEETHANEFEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300FEI FALCON III (4k x 4k)COUNTING43.441C13.0FSC 0.143 CUT-OFFRELION3.0456384OTHERPROJECTION MATCHINGemd_10830_msk_1.mapemd_10830_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
256
256256
0
00256256256212.992212.992212.99290.090.090.0XYZ-0.0283104390.07894012-0.00000785410.0032116970.8320.8320.832emd_10830_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)