2020-09-02RELPDBePDBePDBe2020-04-072020-07-292020-07-292020-09-02Rix1-Rea1 pre-60S particle - Rix1-subcomplex, body 3 (rigid body refinement)Kater LBeckmann RKater LMitterer VThoms MCheng JBerninghausen OBeckmann RHurt EConstruction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.Mol.CellUS79615628.e5202032668200doi:10.1016/j.molcel.2020.06.0321097-27652168MOCEFLEMD-10836associated EM volumeRix1-Rea1 pre-60S particle - Rix1-subcomplex, body 3 (rigid body refinement)6yleFULLOVERLAPRix1-Rea1 pre-60S assembly particle - Rix1-subcomplexRix1-Rea1 pre-60S assembly particle - Rix1-subcomplex0123Rix1-TAP Flag-Rea1 derived pre-60S assembly complexSaccharomyces cerevisiaePre-rRNA-processing protein IPI3Baker's yeast0.0618366952LEVOMDEQVIFTTNTSGTIASVHSFEQINLRQCSTQSRNSCVQVGNKYLFIAQAQKALINVYNLSGSFKRESVEQRLPLPEILK
CLEVVENDGVQYDRIQGVNHNLPDFNLPYLLLGSTESGKLYIWELNSGILLNVKPMAHYQSITKIKSILNGKYIITSGND
SRVIIWQTVDLVSASNDDPKPLCILHDHTLPVTDFQVSSSQGKFLSCTDTKLFTVSQDATIRCYDLSLIGSKKKQKANEN
DVSIGKTPVLLATFTTPYSIKSIVLDPADRACYIGTAEGCFSLNLFYKLKGNAIVNLLQSAGVNTVQKGRVFSLVQRNSL
TGGENEDLDALYAMGQLVCENVLNSNVSCLEISMDGTLLLIGDTEGKVSIAEIYSKQIIRTIQTLTTSQDSVGEVTNLLT
NPYRLERGNLLFEGESKGKQPSNNNGHNFMKIPNLQRVIFDGKNKGHLHDIWYQIGEPEAETDPNLALPLNDFNAYLEQV
KTQESIFSHIGKVSSNVKVIDNKIDATSSLDSNAAKDEEITELKTNIEALTHAYKELRDMHEKLYEEHQQMLDKQPre-rRNA-processing protein RIX1Baker's yeast0.0868398442LEVOMSEEFIAVSTLARNLEIAKGNEFHTILATLRSPVYINEQLLKSELSFLVTKILKLIRSGNDFDLWKGCHTSVVTCAYNPL
VLSTHGGQLLAAIYSRLEQKTGFYSSVISSSHGKQLFNTLISSVAIIIDLMKNKPTLSREALVPKLKAIIPTLITLSQYE
PELVLPVLQRILKRNTTTFKPFTNKFRTVLINLIISDYASLGTKTQRLVCENFAYLHLLKIQVSDTSDDETQAHHKIYAD
SNWRTGLMSILSQFKPIIQLCGEILDFEQDNELYKLIKSLPVIDESNNKEEFLPSLKLDFNAPLTLWEIPQRLSLLADML
VAFISLPTPFPIRVPLGGINSLCEVLLGVSNKYLPLKKELRHDNELNGVINTILPQIQFQGIRLWEIMVSKYGKCGLSFF
EGILSSIELFIPLKKKSNNEIDFNVVGSLKFEFATVFRLVNMILSHLGHQLNIISVISQLIEVALFLSHDKTLIDSLFKN
RKSIMKQQTKTKQSKRSKSAEGAFSDIYTHPELFVCKNSMNWFNEINDFFITALNNWILPSTPHIQILKYSITQSLRLKE
RFGYIPESFVNLLRCEVLHPGSERVSILPIAISLLKNINDDMFELLCHPKVPVGMVYQLHKPLDLGEDGEVRDDINKKEV
ETNESSSNANTGLETLKALENLENVTIPEPKHEVPKVVDDTAIFKKRSVEEVIERESTSSHKKVKFVEETTVDNGEELIV
KKAVSQTKEEEKPMEDSEDEEQEEFEIPAIELSDDEEEEEEEEPre-rRNA-processing protein IPI1Baker's yeast0.0379288951LEVOMTKSRKQKQKKQDFLRKKLKVGKPKEKARNATDTSFVSKTISIRNQHLDQNPHDLTKRLTLLKHHNINVRKETLTTFQKS
IPSIIKSRLMTPLLTQSIPLICDESQQVRQGLIDLVDEIGSHDAEILKLHCNIFVLYINMAMTHIVTQIQADSTKFLSHL
LKYCGDEVVRKSWVKLLNGVFGVLGWGQVGKNDSASIVQTKKRNAKYVTIHLNALYTLVEYGCQDERARSDGDTAETTED
SGTLRNPYLIPDYPQPFEHLKLFTRELKVQDATSSGVNATLLSLATQDIDTRKAVFIEQFLPIVRKKIEVIIKEGGECGK
SANKLKTLLAKIFDsingleParticleparticle7.5Quantifoil R3/3COPPER300CARBONCONTINUOUSGLOW DISCHARGEAIRETHANEFEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 QUANTUM (4k x 4k)INTEGRATING383837105.01-48110.075.01273799Gctf1.06EMD-3199C1FOURIER SPACE3.3FSC 0.143 CUT-OFFRELION3.0.8114398MAXIMUM LIKELIHOODRELION3.0.8MAXIMUM LIKELIHOODRELION3.0.8RELION3.0.8AB INITIO MODELemd_10836_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
200
200200
0
00200200200211.8211.8211.890.090.090.0XYZ-0.0218378210.0463709270.00255409230.00534681841.0591.0591.059Relion half map (unfiltered, unmasked)emd_10836_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)