2022-04-06RELPDBePDBePDBe2020-04-152020-04-292020-04-292022-04-06Medical Research Council (MRC, United Kingdom)MR/N00065X/1United KingdomWellcome Trust101122/Z/13/ZUnited KingdomStructure of the SARS-CoV-2 spike S1 protein in complex with CR3022 FabHuo JZhao YRen JZhou DDuyvesteyn HMECarrique LMalinauskas TRuza RRShah PNMFry EEOwens RStuart DIHuo JZhao YRen JZhou DDuyvesteyn HMEGinn HMCarrique LMalinauskas TRuza RRShah PNMTan TKRijal PCoombes NBewley KRTree JARadecke JPaterson NGSupasa PMongkolsapaya JScreaton GRCarroll MTownsend AFry EEOwens RJStuart DINeutralization of SARS-CoV-2 by Destruction of the Prefusion Spike.Cell Host MicrobeUS28445454.e6202032585135doi:10.1016/j.chom.2020.06.0101934-6069EMD-10863associated EM volumeStructure of the SARS-CoV-2 spike S1 protein in complex with CR3022 Fab6yorFULLOVERLAPBinary complex of SARS-CoV-2 spike S1 with CR3022 FabBinary complex of SARS-CoV-2 spike S1 with CR3022 Fab0123RBD11Severe acute respiratory syndrome coronavirus 2Homo sapiensImmunoblobulin heavy chain12Homo sapiensHomo sapiensImmunoblobulin light chain13Homo sapiensHomo sapiensSpike glycoproteinSevere acute respiratory syndrome coronavirus 20.0227585182Homo sapiensLEVOPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQ
IAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCY
FPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNIgG H chainHomo sapiens0.0244555199999999982Homo sapiensLEVOTQMQLVQSGTEVKKPGESLKISCKGSGYGFITYWIGWVRQMPGKGLEWMGIIYPGDSETRYSPSFQGQVTISADKSINTA
YLQWSSLKASDTAIYYCAGGSGISTPMDVWGQGTTVTVASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWN
SGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKHHHHHHIgG L chainHomo sapiens0.0242898849999999972Homo sapiensLEVODIQLTQSPDSLAVSLGERATINCKSSQSVLYSSINKNYLAWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLT
ISSLQAEDVAVYYCQQYYSTPYTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNAL
QSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECsingleParticleparticle8.02 mM Tris pH 8.0, 200 mM NaCl, 0.02 % NaN3ETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K3 (6k x 4k)1133071.442.014852523Initial from template generated with subset of data.Gctf1.06Default settings in Gctf.6VSB1C1BACK PROJECTION3.3FSC 0.143 CUT-OFFRELION3.1327945MAXIMUM LIKELIHOODcryoSPARC2.14.1-liveMAXIMUM LIKELIHOOD2.14.1-livecryoSPARC2.14.1-live14852523GctfRBD-CR3022 map in this deposition.1C1BACK PROJECTION3.9FSC 0.143 CUT-OFFcryoSPARC2.14.1-live100295ANGULAR RECONSTITUTIONcryoSPARC2.14.1-liveANGULAR RECONSTITUTIONcryoSPARC2.14.1-livecryoSPARC2.14.1-live