2020-09-23RELPDBePDBePDBe2020-07-202020-09-162020-09-162020-09-23European Research Council (ERC)679734FranceFrench National Research AgencyANR-10-LABX-0030-INRTFranceFrench Infrastructure for Integrated Structural Biology (FRISBI)ANR-10-INBS-05FranceEuropean Molecular Biology Organization (EMBO)LTF375-2019FranceFrench National Research AgencyANR-10-IDEX-0002-02FranceE. coli 70S-RNAP expressome complex in NusG-coupled state (42 nt intervening mRNA)Webster MWTakacs MWeixlbaumer AWebster MWTakacs MZhu CVidmar VEduljee AAbdelkareem MWeixlbaumer AStructural basis of transcription-translation coupling and collision in bacteria.ScienceUS36913551359202032820062doi:10.1126/science.abb50361095-92030038SCIEASEMD-11421associated EM volumeE. coli 70S-RNAP expressome complex in NusG-coupled state (42 nt intervening mRNA)6ztnFULLOVERLAPE. coli 70S-RNAP expressome complex in coupled state (42 nt intervening mRNA)E. coli 70S-RNAP expressome complex in coupled state (42 nt intervening mRNA)01234567891011121314151617181920212223242526272829303132333435363738394041424344454647484950515253545556575859606170S ribosome within the MF42-NusG expressome1123456789101112131415161718192021252627282930313233343536373839404142434445464748495051525354Escherichia coliDNA-directed RNA polymerase within the MF42-NusG expressome123245556575861Escherichia coliEscherichia coli BL21(DE3)pEcrpoABC(-XH)Zsynthetic mRNA and template DNA1225960synthetic constructsynthetic construct16S ribosomal RNAEscherichia coli0.4998884061AAAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGC
AGCUUGCUGCUUUGCUGACGAGUGGCGGACGGGUGAGUAAUGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGA
AACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUG
GGAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGA
ACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGC
CGCGUGUAUGAAGAAGGCCUUCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUU
GACGUUACCCGCAGAAGAAGCACCGGCUAACUCCG(PSU)GCCAGCAGCC(G7M)CGGUAAUACGGAGGGUGCAAGCGUU
AAUCGGAAUUACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAGAUGUGAAAUCCCCGGGCUCAACCUGGGAACU
GCAUCUGAUACUGGCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGA
GGAAUACCGGUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCAAACAGGAUUAGA
UACCCUGGUAGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCCUUGAGGCGUGGCUUCCGGAGCUAACGCGUUA
AGUCGACCGCCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGU
GGUUUAAUUCGAU(2MG)(5MC)AACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAGAAUG
UGCCUUCGGGAACCGUGAGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGAAAUGUUGGGUUAAGUCCCGCAACG
AGCGCAACCCUUAUCCUUUGUUGCCAGCGGUCCGGCCGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUG
GGGAUGACGUCAAGUCAUCAUG(2MG)CCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCG
ACCUCGCGAGAGCAAGCGGACCUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACUCGACUCCAUGAAGUCGGAA
UCGCUAGUAAUCGUGGAUCAGAAUGCCACGGUGAAUACGUUCCCGGGCCUUGUACACACCG(4OC)CCGU(5MC)ACACC
AUGGGAGUGGGUUGCAAAAGAAGUAGGUAGCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGA
AGUCG(UR3)AACAAGGUAACCGUAGG(2MG)G(MA6)(MA6)CCUGCGGUUGGAUCACCUCCUUA30S ribosomal protein S2Escherichia coli0.026781671LEVOMATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELNKIASRKGKILFVGTKRAASEAV
KDAALSCDQFFVNHRWLGGMLTNWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVAATVREGRSQDLASQAEESFVEA
E30S ribosomal protein S3Escherichia coli0.0260313161LEVOMGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLTKELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKK
GEDVEKLRKVVADIAGVPAQINIAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPEKPAAQPKKQQRKGRK30S ribosomal protein S4Escherichia coli0.0235141991LEVOMARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAA
RLKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK30S ribosomal protein S5Escherichia coli0.0176174551LEVOMAHIEKQACELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNGRVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGT
LQHPVKGVHTGSRVFMQPASEGTGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPAMVAAKRGKS
VEEILGK30S ribosomal protein S6Escherichia coli0.0151970321LEVOMRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRF
NDAVIRSMVMRTKHAVTEASPMVKAKDERRERRDDFANETADDADAGDSEE30S ribosomal protein S7Escherichia coli0.0176374451LEVOMPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELEAFEVALENVRPTVEVKSRRV
GGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW30S ribosomal protein S8Escherichia coli0.0141465571LEVOMSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEGFIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSR
PGLRIYKRKDELPKVMAGLGIAVVSTSKGVMTDRAARQAGLGGEIICYVA30S ribosomal protein S9Escherichia coli0.014886271LEVOMAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETARMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIR
HGITRALMEYDESLRSELRKAGFVTRDARQVERKKVGLRKARRRPQFSKR30S ribosomal protein S10Escherichia coli0.0117555971LEVOMQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPT
EKTVDALMRLDLAAGVDVQISLG30S ribosomal protein S11Escherichia coli0.0138709751LEVOMAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIK
NLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHNGCRPPKKRRV30S ribosomal protein S12Escherichia coli0.0138142491LEVOMATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVI
LIRGGRVK(D2T)LPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA30S ribosomal protein S13Escherichia coli0.0131284671LEVOMARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVKISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRL
MDLGCYRGLRHRRGLPVRGQRTKTNARTRKGPRKPIKK30S ribosomal protein S14Escherichia coli0.011606561LEVOMAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNAVLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLS
RIKVREAAMRGEIPGLKKASW30S ribosomal protein S15Escherichia coli0.0102908161LEVOMSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQ
LIERLGLRR30S ribosomal protein S16Escherichia coli0.0092075721LEVOMVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIASEKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNK
AA30S ribosomal protein S17Escherichia coli0.0097244911LEVOMTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKLHVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVE
KAVL30S ribosomal protein S18Escherichia coli0.0090054721LEVOMARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRITGTRAKYQRQLARAIKRARYLSLLPYTDRHQ30S ribosomal protein S19Escherichia coli0.0104553551LEVOMPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNMIGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTY
RGHAADKKAKKK30S ribosomal protein S20Escherichia coli0.0097084641LEVOMANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLT
AQINKLA30S ribosomal protein S21Escherichia coli0.0085240391LEVOMPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTERKRAKASAVKRHAKKLARENARRTRLYmRNAsynthetic construct0.018409081ACAAAGGAGGUAUUCAUGUUCACAUACACAUAUAGAAGUAGACACAAACGGCGCGCGtRNA(fmet) P-siteEscherichia coli0.0248329181CGCGGGG(4SU)GGAGCAGCCUGG(H2U)AGCUCGUCGGG(OMC)UCAUAACCCGAAGAUCGUCGG(5MU)(PSU)CAAA
UCCGGCCCCCGCAACCAPhe-NH-tRNA(Phe) A-siteEscherichia coli0.0247510182GCCCGGA(4SU)AGCUCAG(H2U)CGG(H2U)AGAGCAGGGGA(PSU)UGAA(MIA)A(PSU)CCCCGU(7MG)(3AU)C
CUUGG(5MU)(PSU)CGAUUCCGAGUCCGGGCACCA23S ribosomal RNAEscherichia coli0.9418205621GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGUCAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCG
GUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAUGGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGA
AUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAUCUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCC
AGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAUCAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGA
UACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGUGAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUG
AAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAA
GAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUGUACGUACAAGCAGUGGGAGCACAGAAAUCUGUGUGACUGC
GUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUC
UUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCCGGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACU
AACUGGAGGACCGAACCGACUAAU(1MG)(PSU)(5MU)GAAAAAUUAGCGGAUGACUUGUGGCUGGGGGUGAAAGGCCA
AUCAAACCGGGAGAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGUGAAUUCAUCUCCGGGGGUAGAGCACU
GUUUCGGCAAGGGGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUACCGGAGAAUGUUAUCACGGGAGACACAC
GGCGGG(PSU)GCUAACGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUAAGGUCCCAAAGUCAUGGUUAAGUG
GGAAACGAUGUGGGAAGGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCAC
UGGUCGAGUCGGCCUGCGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUGCGGCAGCGACGCUUAUGCGUUGUU
GGGUAGGGGAGCGUUCUGUAAGCCUGUGAAGGUGUGCUGUGAGGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACAUA
AGUAACGAUAAAGCGGGUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCCAACGUUAAUCGGGGCAGGGUGAGU
CGACCCCUAAGGCGAGGCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGGUGUUACUGCGAAGGGG
GGACGGAGAAGGCUAUGUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGCUGGUUUUCCAGGCAAAUCCGGAAA
AUCAAGGCUGAGGCGUGAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUGCUUCCAGGAAAAGCCUCUAAGCAU
CAGGUAACAUCAAAUCGUACCCCAAACCGACAC(6MZ)GGUGGUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCGG
GUGAAGGAACUAGGCAAAAUGGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGUGAGGUCCCUCGCGGAUGGAG
CUGAAAUCAGUCGAAGAUACCAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGCAAACACGAAAGUGGACGUAU
ACGGUGUGACGCCU(2MG)CCCGGUGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCUCUUGAUCGAAGCCCCG
GUAAACGGCGGCCG(PSU)AAC(3TD)A(PSU)AACGGUCCUAAGGUAGCGAAA(5MU)UCCUUGUCGGGUAAGUUCCGA
C(5MC)UGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACCCGAGACUCAGUGAAAUUGAACUCGCUGUG(6MZ)AG
AUGCAGUGUACCCGCGGCAAGACGGAAAGACCCCGU(G7M)AACCUUUACUAUAGCUUGACACUGAACAUUGAGCCUUGA
UGUGUAGGAUAGGUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGCCGACCUUGAAAUACCACCCUUUAAUGUU
UGAUGUUCUAACGUUGACCCGUGAUCCGGGUUGCGGACAGUGUCUGGUGGGUAGUUUGACUG(OMG)GGCGGUCUCCUCC
UAAAGAGUAACGGAGGAGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGUUAGUGCAAUGGCAUAAGCCAGCUU
GACUGCGAGCGUGACGGCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGGUUCUGAAUGGAAGGGCCAUCGCUC
AACGGAUAAAAGGUACUCCG(2MG)GGA(H2U)AACAGGC(PSU)GAUACCGCCCAAGAGUUCAUAUCGACGGCGGUGUU
UGGCA(OMC)CUCG(2MA)(PSU)GUCGGCUCAUCACAUCCUGGGGCUGAAGUAGGUCCCAAGGGUAUGGC(OMU)GUUC
GCCAUUUAAAGUGGUACGCGAGC(PSU)GGGUUUAGAACGUCGUGAGACAG(PSU)(PSU)CGGUCCCUAUCUGCCGUGG
GCGCUGGAGAACUGAGGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAUCACUGGUGUUCGGGUUGUCAUGCC
AAUGGCACUGCCCGGUAGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGCACGAAACUUGCCCCGAGAUGAGUU
CUCCCUGACUCCUUGAGAGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGCCGGGUGUGUAAGCGCAGCGAUGCG
UUGAGCUAACCGGUACUAAUGAACCGUGAGGCUUAACCUU5S ribosomal RNAEscherichia coli0.038790091UGCCUGGCGGCCGUAGCGCGGUGGUCCCACCUGACCCCAUGCCGAACUCAGAAGUGAAACGCCGUAGCGCCGAUGGUAGU
GUGGGGUCUCCCCAUGCGAGAGUAGGGAACUGCCAGGCAU50S ribosomal protein L2Escherichia coli0.0299236191LEVOMAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVER
LEYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNF
GKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK50S ribosomal protein L3Escherichia coli0.0222915621LEVOMIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDGYRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLW
EFRLAEGEEFTVGQSISVELFADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQN(MEQ)TPGKVF
KGKKMAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA50S ribosomal protein L4Escherichia coli0.0221215661LEVOMELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRS
GGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA50S ribosomal protein L5Escherichia coli0.0203336111LEVOMAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIR
QGYPIGCKVTLRGERMWEFFERLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
KSDEEGRALLAAFDFPFRK50S ribosomal protein L6Escherichia coli0.0189327911LEVOMSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVT
EGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
GVRYADEVVRTKEAKKK50S ribosomal protein L9Escherichia coli0.015789021LEVOMQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEARRAELEAKLAEVLAAANARAEKINALETVTI
ASKAGDEGKLFGSIGTRDIADAVTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE50S ribosomal protein L13Escherichia coli0.0160506061LEVOMKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGH
IGGIKQATFEEMIARRPERVIEIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI50S ribosomal protein L14Escherichia coli0.0135650671LEVOMIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFD
GNACVLLNNNSEQPIGTRIFGPVTRELRSEKFMKIISLAPEVL50S ribosomal protein L15Escherichia coli0.0149944431LEVOMRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLS
DLAKVEGGVVDLNTLKAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE50S ribosomal protein L16Escherichia coli0.0153272611LEVOMLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAV
(4D4)MGKGKGNVEYWVALIQPGKVLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM50S ribosomal protein L17Escherichia coli0.0143936571LEVOMRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLF
NELGPRFASRAGGYTRILKCGFRAGDNAPMAYIELVDRSEKAEAAAE50S ribosomal protein L18Escherichia coli0.0127946681LEVOMDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAE
RALEKGIKDVSFDRSGFQYHGRVQALADAAREAGLQF50S ribosomal protein L19Escherichia coli0.0131592781LEVOMSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPV
VDSISVKRRGAVRKAKLYYLRERTGKAARIKERLN50S ribosomal protein L20Escherichia coli0.0135280241LEVOMARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFI
NGLKKASVEIDRKILADIAVFDKVAFTALVEKAKAALA50S ribosomal protein L21Escherichia coli0.0115863741LEVOMYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRR
KHYRKQQGHRQWFTDVKITGISA50S ribosomal protein L22Escherichia coli0.0122533591LEVOMETIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGP
SMKRIMPRAKGRADRILKRTSHITVVVSDR50S ribosomal protein L23Escherichia coli0.011222161LEVOMIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDW
KKAYVTLKEGQNLDFVGGAE50S ribosomal protein L24Escherichia coli0.011339251LEVOMAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKA
DRVGFRFEDGKKVRFFKSNSETIK50S ribosomal protein L25Escherichia coli0.0107134651LEVOMFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDHDKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRH
PYKPKLQHIDFVRA50S ribosomal protein L27Escherichia coli0.009146541LEVOMAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFI
SIEAE50S ribosomal protein L28Escherichia coli0.0090275511LEVOMSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEKRFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY50S ribosomal protein L29Escherichia coli0.0072864641LEVOMKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAGA50S ribosomal protein L30Escherichia coli0.006554821LEVOMAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAVSFMVKVEE50S ribosomal protein L32Escherichia coli0.0064634451LEVOMAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK50S ribosomal protein L33Escherichia coli0.0063886311LEVOMAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVRQHVIYKEAKIK50S ribosomal protein L34Escherichia coli0.0053974631LEVOMKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK50S ribosomal protein L35Escherichia coli0.0073130321LEVOMPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHLRPKAMVSKGDLGLVIACLPYA50S ribosomal protein L36Escherichia coli0.005720061LEVOMKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQGLIFSHIFLAKLGDNA-directed RNA polymerase subunit alphaEscherichia coli0.036558682Escherichia coli BL21(DE3)LEVOMQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILE
ILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLRDVRQP
EVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLEN
WPPASIADE2.7.7.6DNA-directed RNA polymerase subunit betaEscherichia coli0.1508208751Escherichia coli BL21(DE3)LEVOMVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVF
DVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQME
LVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLD
LLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAV
GRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLS
LGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCP
IETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKG
ESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKR
GGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNM
RVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGG
DILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDL
SEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGD
DLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMA
AKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLK
LGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEA
YGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNVLLKEIRSLGINIELEDE2.7.7.6DNA-directed RNA polymerase subunit beta'Escherichia coli0.1553667811Escherichia coli BL21(DE3)LEVOMKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKH
RGVICEKCGVEVTQTKVRRERMGHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILT
VLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSN
KRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKM
VEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEA
RALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDAN
GELVAKTSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVG
IDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFN
SIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRL
VDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADILVPRNTLLHEQWCDLLEENSVDAV
KVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKL
SNVKSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITEVSGFVRFTDM
IDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDVLIPGTDMPAQYFLPGKAIVQLEDGVQISSGDT
LARIPQESGGTKDITGGLPRVADLFEARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFE
GERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKATIVNAGSSDFLEGEQV
EYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFISAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAG
TGYAYHQDRMRRRAAGEAPAAPQVTAEDASASLAELLNAGLGGSDNE2.7.7.6DNA-directed RNA polymerase subunit omegaEscherichia coli0.0102495471Escherichia coli BL21(DE3)LEVOMARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALREIEEGLINNQILDVRERQEQQEQEAAEL
QAVTAIAEGRR2.7.7.6Non-template DNA strandsynthetic construct0.0120637541(DG)(DG)(DT)(DC)(DA)(DG)(DT)(DA)(DC)(DG)(DT)(DC)(DC)(DT)(DA)(DT)(DC)(DG)(DA)(DT)
(DC)(DT)(DT)(DC)(DG)(DG)(DA)(DA)(DG)(DA)(DG)(DA)(DT)(DT)(DC)(DA)(DG)(DA)(DG)DNATemplate DNA strandsynthetic construct0.0118725961(DC)(DT)(DC)(DT)(DG)(DA)(DA)(DT)(DC)(DT)(DC)(DT)(DT)(DC)(DC)(DC)(DG)(DC)(DG)(DC)
(DG)(DC)(DC)(DG)(DT)(DA)(DG)(DG)(DA)(DC)(DG)(DT)(DA)(DC)(DT)(DG)(DA)(DC)(DC)DNATranscription termination/antitermination protein NusGEscherichia coli0.0205605231Escherichia coli BL21(DE3)LEVOMSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMVPTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASW
HLVRSVPRVMGFIGGTSDRPAPISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
SVSIFGRATPVELDFSQVEKAMAGNESIUM ION2.4305e-05487MGPHENYLALANINE0.0001651891PHEZINC ION6.5409e-052ZNsingleParticleparticle810.0HEPESHEPES150.0KOAcPotassium acetate5.0Mg(OAc)2Magneium acetate10.0NH4ClAmmonium chloride2.0ZnCl2Zinc chloride2.0DTTDithiothreitolQuantifoil R2/2COPPER300GLOW DISCHARGEAIRETHANE90283FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3000.73.5FEI TITAN KRIOS AUTOGRID HOLDERGATAN K2 SUMMIT (4k x 4k)COUNTING42.01314705Gctf0.563.9FSC 0.143 CUT-OFFRELION3.0.434590MAXIMUM LIKELIHOODRELION3.0.4MAXIMUM LIKELIHOODRELION3.0.4Particle selection was performed by analysis of RNAP-70S relative orientation.4YBB6ALHRIGID BODY FITCorrelation coefficientREALemd_11421_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
416
416416
0
00416416416723.84723.84723.8490.090.090.0XYZ-0.324991850.384206650.00098286270.0298729821.741.741.74NusG-coupled E. coli expressome with intervening mRNA length 42 nucleotides: half map 2 of expressome refinement.emd_11421_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
416
416416
0
00416416416723.84723.84723.8490.090.090.0XYZ-0.0286878570.078005510.0009774010.0047976641.741.741.74NusG-coupled E. coli expressome with intervening mRNA length 42 nucleotides: expressome map non-composite.emd_11421_additional_3.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
416
416416
0
00416416416723.84723.84723.8490.090.090.0XYZ-0.147474591.971240.00546437550.0530366381.741.741.74NusG-coupled E. coli expressome with intervening mRNA length 42 nucleotides: composite map with local density sharpening.emd_11421_additional_4.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
416
416416
0
00416416416723.84723.84723.8490.090.090.0XYZ-0.367057140.38279810.0009854350.027577231.741.741.74NusG-coupled E. coli expressome with intervening mRNA length 42 nucleotides: half map 1 of expressome refinement.emd_11421_additional_5.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
416
416416
0
00416416416723.84723.84723.8490.090.090.0XYZ-0.103480250.255765020.000295205160.0109608231.741.741.74NusG-coupled E. coli expressome with intervening mRNA length 42 nucleotides: focused refinement 1 of 2 (70S masked).emd_11421_additional_6.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
288
288288
0
00288288288501.12501.12501.1290.090.090.0XYZ-0.062753930.101727280.00147913270.0077820771.741.741.74NusG-coupled E. coli expressome with intervening mRNA length 42 nucleotides: half map 1 of focused refinement 2 of 2 (RNAP masked).emd_11421_additional_7.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
288
288288
0
00288288288501.12501.12501.1290.090.090.0XYZ-0.0378984470.1066404950.000055707520.00300569371.741.741.74NusG-coupled E. coli expressome with intervening mRNA length 42 nucleotides: focused refinement 2 of 2 (RNAP masked).emd_11421_additional_8.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
288
288288
0
00288288288501.12501.12501.1290.090.090.0XYZ-0.0571490340.096508530.00335368770.0074767241.741.741.74NusG-coupled E. coli expressome with intervening mRNA length 42 nucleotides: half map 2 of focused refinement 2 of 2 (RNAP masked).emd_11421_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
416
416416
0
00416416416723.84723.84723.8490.090.090.0XYZ-0.0533796620.172177080.00093680590.007851931.741.741.74NusG-coupled E. coli expressome with intervening mRNA length 42 nucleotides: half map 2 of focused refinement 1 of 2 (70S masked).emd_11421_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
416
416416
0
00416416416723.84723.84723.8490.090.090.0XYZ-0.0511823970.172595250.000874400260.0079519571.741.741.74NusG-coupled E. coli expressome with intervening mRNA length 42 nucleotides: half map 1 of focused refinement 1 of 2 (70S masked).