2021-01-13RELPDBePDBePDBe2020-10-222020-12-022020-12-022021-01-13Austrian Science FundP33367AustriaTomogram of the actin network in an extracted and fixed mouse fibroblast lamellipodium.Faessler FDimchev GHodirnau VVWan WSchur FKMFassler FDimchev GHodirnau VVWan WSchur FKMCryo-electron tomography structure of Arp2/3 complex in cells reveals new insights into the branch junction.Nat CommunUK1164376437202033353942doi:10.1038/s41467-020-20286-x2041-1723EMD-11869other EM volumeD_1292111822 contains the structure and model associated with this entryEMD-11870associated EM volumeActin network in a mouse fibroblast lamellipodiumActin network in a mouse fibroblast lamellipodium012345678Branched actin network of extracted and fixed mouse fibroblast lamellipodiumMus musculustomographycell6.110.0MES150.0sodium chloride5.0EGTA5.0Glucose5.0Magnesium chlorideAdjust to pH 6.1 using NaOHQuantifoil R2/2GOLD200CARBONHOLEYGLOW DISCHARGEAIRAfter glow discharging of the grid and prior to the seeding of cells, the grid was coated using 25ug/ml FibronectinETHANE80277Leica GP2, 3,5sec back-blotting, sensor on, 0,1mm movement after contact, manually pre-blotted within the chamber prior to the application of fiducials. AURION10NO SECTIONINGFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30050.02.7-0.00175-0.005542000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGIF Bioquantum20GATAN K3 BIOQUANTUM (6k x 4k)611.212.79Images were collected in movie-mode with 7 frames per tilt1BACK PROJECTIONIMOD4.9.1261