2021-04-21RELPDBePDBePDBe2020-11-062021-04-142021-04-142021-04-21Cancer Research UKFC001179United KingdomMedical Research Council (MRC, United Kingdom)FC001179United KingdomWellcome TrustFC001179United KingdomMedical Research Council (MRC, United Kingdom)MR/P019374/1United KingdomSctV (SsaV) cytoplasmic domainMatthews-Palmer TRSGonzalez-Rodriguez NCalcraft TLagercrantz SZachs TYu XJGrabe GHolden DNans ARosenthal PRouse SBeeby MMatthews-Palmer TRSGonzalez-Rodriguez NCalcraft TLagercrantz SZachs TYu XJGrabe GJHolden DWNans ARosenthal PBRouse SLBeeby MStructure of the cytoplasmic domain of SctV (SsaV) from the Salmonella SPI-2 injectisome and implications for a pH sensing mechanism.J.Struct.Biol.US213107729107729202133774138doi:10.1016/j.jsb.2021.1077291095-86570803JSBIEMEMD-11928associated EM volumeSctV (SsaV) cytoplasmic domain7awaFULLOVERLAPHomo-nonameric ring complex of SsaV (SctV) type III secretion system export apparatus protein.Homo-nonameric ring complex of SsaV (SctV) type III secretion system export apparatus protein.01Full length protein including transmembrane domain recombinantly expressed in E.coli C41 and extracted from membrane fraction with detergent DDM.
Transmembrane domain present but not resolved.Salmonella entericaLT20.68Escherichia coliC41(DE3)pQlinkN-ssaV6hisSecretion system apparatus protein SsaVSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)LT2 / SGSC1412 / ATCC 7007200.076221358999999991Escherichia coli BL21(DE3)LEVOMRSWLGEGVRAQQWLSVCAGRQDMVLATVLLIAIVMMLLPLPTWMVDILITINLMFSVILLLIAIYLSDPLDLSVFPSLL
LITTLYRLSLTISTSRLVLLQHNAGNIVDAFGKFVVGGNLTVGLVVFTIITIVQFIVITKGIERVAEVSARFSLDGMPGK
QMSIDGDLRAGVIDADHARTLRQHVQQESRFLGAMDGAMKFVKGDTIAGIIVVLVNIIGGIIIAIVQYDMSMSEAVHTYS
VLSIGDGLCGQIPSLLISLSAGIIVTRVPGEKRQNLATELSSQIARQPQSLILTAVVLMLLALIPGFPFITLAFFSALLA
LPIILIRRKKSVVSANGVEAPEKDSMVPGACPLILRLSPTLHSADLIRDIDAMRWFLFEDTGVPLPEVNIEVLPEPTEKL
TVLLYQEPVFSLSIPAQADYLLIGADASVVGDSQTLPNGMGQICWLTKDMAHKAQGFGLDVFAGSQRISALLKCVLLRHM
GEFIGVQETRYLMNAMEKNYSELVKELQRQLPINKIAETLQRLVSERVSIRDLRLIFGTLIDWAPREKDVLMLTEYVRIA
LRRHILRRLNPEGKPLPILRIGEGIENLVRESIRQTAMGTYTALSSRHKTQILQLIEQALKQSAKLFIVTSVDTRRFLRK
ITEATLFDVPILSWQELGEESLIQVVESIDLSEEELADNEEHHHHHHsingleParticleparticle18.050.0Tris-HCl50.0NaCl0.03n-Dodecyl-beta-D-maltoside5.0DithiothreitolQuantifoil R2/2COPPER200CARBONCONTINUOUS0.30000000000000004CARBONHOLEY12.0GLOW DISCHARGEAIRETHANE-PROPANE100277FEI VITROBOT MARK IVblot 2.5s. Sample was monodisperse on a continuous carbon film.TFS KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300NITROGENGIF Bioquantum20GATAN K2 SUMMIT (4k x 4k)COUNTING2672912.070.01988000interactive training particle picker in Xmipp, used in the Scipion wrapperCTFFINDAb initio 3D model generation from a balanced set of 2D views using stochastic gradient descent implemented in Relion. Initial model heavily low-pass filtered.C93.5FSC 0.143 CUT-OFFRELION3.0361000MAXIMUM LIKELIHOODRELIONMAXIMUM LIKELIHOODRELIONOTHERemd_11928_msk_1.map