2021-05-26RELPDBePDBePDBe2020-12-032021-05-262021-05-262021-05-26German Research Foundation (DFG)GermanyE. coli 70S containing suppressor tRNA in the A-site stabilized by a Negamycin analogue and P-site tRNA-nascent chain.Albers SBeckert BMatthies MSchuster RRiedner MSeuring CSanyal STorda AWilson NDIgnatova ZAlbers SBeckert BWilson NDIgnatova ZHarnessing evolutionarily flexibility in tRNAs to maximize nonsense suppression activitiesNat CommunUK20212041-1723EMD-12035associated EM volumeE. coli 70S containing suppressor tRNA in the A-site stabilized by a Negamycin analogue and P-site tRNA-nascent chain.7b5kFULLOVERLAP70S ribosome with A,P,E-site tRNA70S ribosome with A,P,E-site tRNA012345678910111213141516171819202122232425262728293031323334353637383940414243444546474849505152535455565770S ribosome with A,P,E-site tRNA11234567891011121314151617181920212223242526272829303132333435363738394041424344454647484950515253545556Escherichia coli K-12mRNA157Escherichia coli K-12synthetic construct23S rRNAEscherichia coli K-120.939607251GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGUCAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCG
GUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAUGGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGA
AUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAUCUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCC
AGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAUCAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGA
UACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGUGAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUG
AAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAA
GAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUGUACGUACAAGCAGUGGGAGCACGCUUAGGCGUGUGACUGC
GUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUC
UUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCCGGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACU
AACUGGAGGACCGAACCGACUAAU(1MG)(PSU)(5MU)GAAAAAUUAGCGGAUGACUUGUGGCUGGGGGUGAAAGGCCA
AUCAAACCGGGAGAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGUGAAUUCAUCUCCGGGGGUAGAGCACU
GUUUCGGCAAGGGGGUCACUUACCAACCCGAUGCAAACUGCGAAUACCGGAGAAUGUUAUCACGGGAGACACACGGCGGG
(PSU)GCUAACGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUAAGGUCCCAAAGUCAUGGUUAAGUGGGAAAC
GAUGUGGGAAGGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCG
AGUCGGCCUGCGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUGCGGCAGCGACGCUUAUGCGUUGUUGGGUAG
GGGAGCGUUCUGUAAGCCUGCGAAGGUGUGCUGUGAGGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACAUAAGUAAC
GAUAAAGCGGGUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCCAACGUUAAUCGGGGCAGGGUGAGUCGACCC
CUAAGGCGAGGCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGGUGUUACUGCGAAGGGGGGACGG
AGAAGGCUAUGUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGCUGGUUUUCCAGGCAAAUCCGGAAAAUCAAG
GCUGAGGCGUGAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUGCUUCCAGGAAAAGCCUCUAAGCAUCAGGUA
ACAUCAAAUCGUACCCCAAACCGACAC(6MZ)GGUGGUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCGGGUGAAG
GAACUAGGCAAAAUGGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGUGAGGUCCCUCGCGGAUGGAGCUGAAA
UCAGUCGAAGAUACCAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGCAAACACGAAAGUGGACGUAUACGGUG
UGACGCCU(2MG)CCCGGUGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCUCUUGAUCGAAGCCCCGGUAAAC
GGCGGCCG(PSU)AAC(3TD)A(PSU)AACGGUCCUAAGGUAGCGAAA(5MU)UCCUUGUCGGGUAAGUUCCGAC(5MC)
UGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACCCGAGACUCAGUGAAAUUGAACUCGCUGUG(6MZ)AGAUGCAG
UGUACCCGCGGCAAGACGGAAAGACCCCGU(G7M)AACCUUUACUAUAGCUUGACACUGAACAUUGAGCCUUGAUGUGUA
GGAUAGGUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGCCGACCUUGAAAUACCACCCUUUAAUGUUUGAUGU
UCUAACGUUGACCCGUAAUCCGGGUUGCGGACAGUGUCUGGUGGGUAGUUUGACUG(OMG)GGCGGUCUCCUCCUAAAGA
GUAACGGAGGAGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGUUAGUGCAAUGGCAUAAGCCAGCUUGACUGC
GAGCGUGACGGCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGGUUCUGAAUGGAAGGGCCAUCGCUCAACGGA
UAAAAGGUACUCCG(2MG)GGAUAACAGGC(PSU)GAUACCGCCCAAGAGUUCAUAUCGACGGCGGUGUUUGGCA(OMC)
CUCG(2MA)(PSU)GUCGGCUCAUCACAUCCUGGGGCUGAAGUAGGUCCCAAGGGUAUGGC(OMU)GUUCGCCAUUUAAA
GUGGUACGCGAGC(PSU)GGGUUUAGAACGUCGUGAGACAG(PSU)(PSU)CGGUCCCUAUCUGCCGUGGGCGCUGGAGA
ACUGAGGGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAUCACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUG
CCCGGUAGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGCACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACC
CUUUAAGGGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGCCGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAAC
CGGUACUAAUGAACCGUGAGGCUUAACCU5S rRNAEscherichia coli K-120.038790089999999991UGCCUGGCGGCCGUAGCGCGGUGGUCCCACCUGACCCCAUGCCGAACUCAGAAGUGAAACGCCGUAGCGCCGAUGGUAGU
GUGGGGUCUCCCCAUGCGAGAGUAGGGAACUGCCAGGCAU50S ribosomal protein L2Escherichia coli K-120.0296632441LEVOAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERL
EYDPNRSANIALVLYKDGERRYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGTY
VQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFG
KHPVTPWGVQTKGKKTRSNKRTDKFIVRRRS50S ribosomal protein L3Escherichia coli K-120.0222915621LEVOMIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDGYRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLW
EFRLAEGEEFTVGQSISVELFADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQN(MEQ)TPGKVF
KGKKMAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA50S ribosomal protein L4Escherichia coli K-120.0221215661LEVOMELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTRAQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRS
GGVTFAARPQDHSQKVNKKMYRGALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA50S ribosomal protein L5Escherichia coli K-120.0200732341LEVOAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQ
GYPIGCKVTLRGERMWEFFERLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTAK
SDEEGRALLAAFDFPFR50S ribosomal protein L6Escherichia coli K-120.0188015981LEVOSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTE
GFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKG
VRYADEVVRTKEAKKK50S ribosomal protein L9Escherichia coli K-120.015789021LEVOMQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEARRAELEAKLAEVLAAANARAEKINALETVTI
ASKAGDEGKLFGSIGTRDIADAVTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE50S ribosomal protein L13Escherichia coli K-120.0160506061LEVOMKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGH
IGGIKQATFEEMIARRPERVIEIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI50S ribosomal protein L14Escherichia coli K-120.0135650669999999981LEVOMIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFD
GNACVLLNNNSEQPIGTRIFGPVTRELRSEKFMKIISLAPEVL50S ribosomal protein L15Escherichia coli K-120.0150084709999999991LEVOMRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEIRLS
DLAKVEGGVVDLNTLKAANIIGIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE50S ribosomal protein L16Escherichia coli K-120.0153272611LEVOMLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAV
(4D4)MGKGKGNVEYWVALIQPGKVLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM50S ribosomal protein L17Escherichia coli K-120.0135057459999999991LEVOMRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKELRRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLF
NELGPRFASRAGGYTRILKCGFRAGDNAPMAYIELVDR50S ribosomal protein L18Escherichia coli K-120.0127946679999999991LEVOMDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAE
RALEKGIKDVSFDRSGFQYHGRVQALADAAREAGLQF50S ribosomal protein L19Escherichia coli K-120.0130280821LEVOSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVV
DSISVKRRGAVRKAKLYYLRERTGKAARIKERLN50S ribosomal protein L20Escherichia coli K-120.0133968281LEVOARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFIN
GLKKASVEIDRKILADIAVFDKVAFTALVEKAKAALA50S ribosomal protein L21Escherichia coli K-120.0115863741LEVOMYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRR
KHYRKQQGHRQWFTDVKITGISA50S ribosomal protein L22Escherichia coli K-120.0122533591LEVOMETIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGP
SMKRIMPRAKGRADRILKRTSHITVVVSDR50S ribosomal protein L23Escherichia coli K-120.0105464721LEVOMIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIKAAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDW
KKAYVTLKEGQNL50S ribosomal protein L24Escherichia coli K-120.0110788739999999991LEVOAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKKHQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKAD
RVGFRFEDGKKVRFFKSNSETI50S ribosomal protein L25Escherichia coli K-120.0107134651LEVOMFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDHDKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRH
PYKPKLQHIDFVRA50S ribosomal protein L27Escherichia coli K-120.0082754981LEVOTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE50S ribosomal protein L28Escherichia coli K-120.0088963539999999991LEVOSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEKRFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY50S ribosomal protein L29Escherichia coli K-120.0071552671LEVOKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLKQVRRDVARVKTLLNEKAGA50S ribosomal protein L30Escherichia coli K-120.0064236251LEVOAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAIRGMINAVSFMVKVEE50S ribosomal protein L32Escherichia coli K-120.0063322491LEVOAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHITADGYYRGRKVIAK50S ribosomal protein L33Escherichia coli K-120.0059281LEVOGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVRQHVIYKEAKI50S ribosomal protein L34Escherichia coli K-120.0053974631LEVOMKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTVSK50S ribosomal protein L35Escherichia coli K-120.0071818351LEVOPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHLRPKAMVSKGDLGLVIACLPYA50S ribosomal protein L36Escherichia coli K-120.004377391LEVOMKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG50S ribosomal protein L31Escherichia coli K-120.0075166931LEVOMKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFFTGKQRDVATGGRVDRFNKRFNI16S rRNAEscherichia coli K-120.497074749999999951AAAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGA
AGCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAAUGUCUGGGAAACUGCCCGAUGGAGGGGGAUAACUACUGGA
AACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUG
GGAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGA
ACUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGC
CGCGUGUAUGAAGAAGGCCUUCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUU
GACGUUACCCGCAGAAGAAGCACCGGCUAACUCCG(PSU)GCCAGCAGCC(G7M)CGGUAAUACGGAGGGUGCAAGCGUU
AAUCGGAAUUACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAGAUGUGAAAUCCCCGGGCUCAACCUGGGAACU
GCAUCUGAUACUGGCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGA
GGAAUACCGGUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCAAACAGGAUUAGA
UACCCUGGUAGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCCUUGAGGCGUGGCUUCCGGAGCUAACGCGUUA
AGUCGACCGCCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGU
GGUUUAAUUCGAU(2MG)(5MC)AACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAGAAUG
UGCCUUCGGGAACCGUGAGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGAAAUGUUGGGUUAAGUCCCGCAACG
AGCGCAACCCUUAUCCUUUGUUGCCAGCGGUCCGGCCGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUG
GGGAUGACGUCAAGUCAUCAUG(2MG)CCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCG
ACCUCGCGAGAGCAAGCGGACCUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACUCGACUCCAUGAAGUCGGAA
UCGCUAGUAAUCGUGGAUCAGAAUGCCACGGUGAAUACGUUCCCGGGCCUUGUACACACCG(4OC)CCGU(5MC)ACACC
AUGGGAGUGGGUUGCAAAAGAAGUAGGUAGCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGA
AGUCG(UR3)AACAAGGUAACCGUAGG(2MG)G(MA6)(MA6)CCUGCGGUUGGAUC30S ribosomal protein S2Escherichia coli K-120.0249717639999999971LEVOVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLEKTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDA
ALSCDQFFVNHRWLGGMLTNWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDALFV
IDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVAATVREGRSQ30S ribosomal protein S3Escherichia coli K-120.0230787849999999971LEVOGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLTKELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKG
EDVEKLRKVVADIAGVPAQINIAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGAE
IARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEI30S ribosomal protein S4Escherichia coli K-120.0233830021LEVOARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAAR
LKGNTGENLLALLEGRLDNVVYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALEL
AEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK30S ribosomal protein S5Escherichia coli K-120.0163618781LEVOELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNGRVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVH
TGSRVFMQPASEGTGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKSVEEI30S ribosomal protein S6Escherichia coli K-120.0123262511LEVOMRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRF
NDAVIRSMVMRTKHAVTEASPMVKAK30S ribosomal protein S7Escherichia coli K-120.0168615231LEVOPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVG
GSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFA30S ribosomal protein S8Escherichia coli K-120.0140153611LEVOSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEGFIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRP
GLRIYKRKDELPKVMAGLGIAVVSTSKGVMTDRAARQAGLGGEIICYVA30S ribosomal protein S9Escherichia coli K-120.0145548819999999981LEVONQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETARMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGI
TRALMEYDESLRSELRKAGFVTRDARQVERKKVGLRKARRRPQFSKR30S ribosomal protein S10Escherichia coli K-120.0113251169999999991LEVOQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPTRKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKT
VDALMRLDLAAGVDVQISL30S ribosomal protein S11Escherichia coli K-120.01248721LEVORKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPG
RESTIRALNAAGFRITNITDVTPIPHNGCRPPKKRRV30S ribosomal protein S12Escherichia coli K-120.0136830529999999991LEVOATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVIL
IRGGRVK(D2T)LPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA30S ribosomal protein S13Escherichia coli K-120.0126257531LEVOARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVKISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLM
DLGCYRGLRHRRGLPVRGQRTKTNARTRKGPRKP30S ribosomal protein S14Escherichia coli K-120.0115464419999999991LEVOAKQSMKAREVKRVALADKYFAKRAELKAIISDVNAASDEDRWNAVLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLS
RIKVREAAMRGEIPGLKKASW30S ribosomal protein S15Escherichia coli K-120.0101596209999999991LEVOSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQL
IERLGLRR30S ribosomal protein S16Escherichia coli K-120.0092075719999999991LEVOMVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIASEKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNK
AA30S ribosomal protein S17Escherichia coli K-120.0092639461LEVOKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKLHVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAV30S ribosomal protein S18Escherichia coli K-120.0064664771LEVOEIDYKDIATLKNYITESGKIVPSRITGTRAKYQRQLARAIKRARYLSLLPYTDRH30S ribosomal protein S19Escherichia coli K-120.0093239021LEVORSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNMIGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRG
HA30S ribosomal protein S20Escherichia coli K-120.0095772681LEVOANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTA
QINKLA30S ribosomal protein S21Escherichia coli K-120.0066297439999999991LEVOPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTERKRAKASAVKRHAermC Nacent chainEscherichia coli K-120.0008950971LEVOGIFSIFVIP-site Isoleucine tRNA (77-MER)Escherichia coli K-120.0248397541AGGCUUAUAGCUCAGGUGGUUAGAGCGCACCCCUGAUAAGGGUGAGGUCGGUGGUUCAAGUCCACUCAGGCCUACCAatRNA synthetic Stop tRNA (76-MER)Escherichia coli K-120.0246326929999999971GGGGUGGUGGCUCAGAAGGGAGAGCAGCGGCCUUCAGAGCCGCGAGACGGGGGUUCGAUUCCCCUCCGCUCCACCAE-site val tRNA (76-MER)Escherichia coli K-120.0244445311GGGUGAUUAGCUCAGCUGGGAGAGCACCUCCCUUACAAGGAGGUGGUCGGCGGUUCGAUCCCGUCAUCACCCACCAmRNAEscherichia coli K-120.0034911381GUAAUCUGAACMAGNESIUM ION2.4305e-05359MGERYTHROMYCIN A0.0007339271ERY2-[[[(3~{R},5~{R})-3-azanyl-6-(3-azanylpropylamino)-5-oxidanyl-hexanoyl]amino]-methyl-amino]ethanoic acid0.0003053742SY5water1.8015e-05854HOHsingleParticleparticle7.5QuantifoilCOPPER300CARBONHOLEY3.0GLOW DISCHARGEETHANE-PROPANE95FEI VITROBOT MARK IVFEI TALOS ARCTICASPOT SCANBRIGHT FIELDFIELD EMISSION GUN2002.7FEI FALCON III (4k x 4k)COUNTING160040.01.01GctfC12.9FSC 0.143 CUT-OFFRELION3.145000MAXIMUM LIKELIHOODRELION3.1MAXIMUM LIKELIHOODRELION3.1RELION3.1RIGID BODY FITREALemd_12035_additional_4.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
360
360360
0
00360360360345.6345.6345.690.090.090.0XYZ-0.0169613360.128340420.00323454550.0118342770.960000040.960000040.96000004relion final 3D refineemd_12035_additional_3.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
360
360360
0
00360360360345.6345.6345.690.090.090.0XYZ-0.109091220.2706040.00131594950.006889540.960000040.960000040.96000004Multi body SSU-heademd_12035_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
360
360360
0
00360360360345.6345.6345.690.090.090.0XYZ-0.160986450.313755420.0014107330.0091738170.960000040.960000040.96000004Multi body SSU-bodyemd_12035_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
360
360360
0
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