2021-04-28RELPDBePDBePDBe2021-02-042021-04-072021-04-072021-04-28Max Planck SocietyGermanyWellcome Trust201543/Z/16/ZUnited KingdomEuropean Research Council (ERC)856118European UnionMedical Research Council (MRC, United Kingdom)MR/R003106/1United KingdomEuropean Molecular Biology Organization (EMBO)ALTF 693-2018European UnionIn situ structure of the I-band thin filament including troponin complex from mouse psoas muscleWang ZGrange MWagner TKho ALGautel MRaunser SWang ZGrange MWagner TKho ALGautel MRaunser SThe molecular basis for sarcomere organization in vertebrate skeletal muscle.Cell18421352150.e13202133765442doi:10.1016/j.cell.2021.02.0471097-4172EMD-12292other EM volumeEMD-12291other EM volumeEMD-12289other EM volumeEMD-12293associated EM volumeI-band thin filament including troponin from mouse psoas muscleI-band thin filament including troponin from mouse psoas muscle0123456Mus musculusactin11Mus musculustropomyosin1456Mus musculustroponin1Mus musculussubtomogramAveragingcell7.020.0C8H18N2O4SHEPES140.0KClpotassium chloride2.0MgCl2magnesium chloride1.0EGTAegtazic acid1.0DTTdithiothreitolQuantifoilCOPPER200CARBONHOLEYGLOW DISCHARGEAIRETHANE100286FEI VITROBOT MARK IVThe sample was myofibrils.FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30050.02.781000.028409.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)COUNTING371038381-83.51C1BACK PROJECTION19.8FSC 0.143 CUT-OFFRELION3.0.47044704IMODRELION3.0.4MAXIMUM LIKELIHOODRELION3.0.46KN8RIGID BODY FITemd_12293_msk_1.mapemd_12293_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
160
160160
0
00160160160280.8280.8280.890.090.090.0XYZ-2.04721242.20980626.6062436e-050.237548661.75499991.75499991.7549999emd_12293_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)