REL
PDBe
PDBe
PDBe
2006-06-23
2006-07-26
2006-07-26
2014-03-19
The cryo-EM structure of a translation initiation complex from Escherichia coli.
Allen GS
Zavialov A
Gursky R
Ehrenberg M
Frank J
Allen GS
Zavialov A
Gursky R
Ehrenberg M
Frank J
The cryo-EM structure of a translation initiation complex from Escherichia coli.
CELL(CAMBRIDGE,MASS.)
121
703
712
2005
15935757
doi:10.1016/j.cell.2005.03.023
1zo1
FULLOVERLAP
1zo3
FULLOVERLAP
E. coli 70S, IF1, IF3, mRNA, fMet-tRNA, and IF2-GDPNP
E. coli 70S, IF1, IF3, mRNA, fMet-tRNA, and IF2-GDPNP
One of each component binds to the 70S
7
30S
false
Escherichia coli
ribosome-prokaryote: SSU 30S
50s
false
Escherichia coli
ribosome-prokaryote: LSU 50S
fmet-tRNA
Escherichia coli
TRANSFER
DOUBLE HELIX
false
beta IF2-GDPNP
Escherichia coli
1
monomer
true
Escherichia coli
IF1
Escherichia coli
1
1
true
Escherichia coli
IF3
Escherichia coli
1
1
true
Escherichia coli
mRNA
Escherichia coli
OTHER
SINGLE STRANDED
true
singleParticle
particle
7.6
polymix buffer
Quantifoil
ETHANE
90
100
OTHER
Vitrification instrument: Vitrobot
2 second blot
FEI TECNAI F30
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
2.26
0.93
3.93
39000.0
39000.0
GATAN HELIUM
80
2003-07-04
KODAK SO-163 FILM
ZEISS SCAI
14
116
1.2
12.
cryo stage
automated particle picking followed by manual verification
defocus groups
C1
OTHER
13.8
FSC 0.5 CUT-OFF
SPIDER
The falloff of Fourier amplitudes toward higher spatial
frequencies was corrected using the x-ray solution scattering
intensity distribution of 70S ribosomes from E. coli during each
round of refinement.
20283
RIGID BODY FIT
RSRef
Protocol: real-space refinement of rigid bodies. IF2 and IF1 were initially fit by hand in O then refined by RSRef
correlation coefficient
REAL