2021-06-16RELPDBePDBePDBe2021-03-052021-05-052021-05-052021-06-16European Research Council (ERC)789016-NEDD8ActivateGermanyGerman Research Foundation (DFG)SCHU 3196/1-1GermanySupramolecular assembly module of yeast Chelator-GID SR4 E3 ubiquitin ligaseChrustowicz JSherpa DPrabu JRSchulman BASherpa DChrustowicz JQiao SLanglois CRHehl LAGottemukkala KVHansen FMKarayel Ovon Gronau SPrabu JRMann MAlpi AFSchulman BAGID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme.Mol.CellUS812445202133905682doi:10.1016/j.molcel.2021.03.0251097-27652168MOCEFLSherpa DChrustowicz JQiao SLanglois CRHehl LAGottemukkala KVHansen FMKarayel OPrabu JRMann MAlpi AFSchulman BAGID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzymeBiorxivUS2021doi:10.1101/2021.04.07.436316EMD-12541other EM volumeLow resolution map of Apo Chelator-GID SR4EMD-12557other EM volumeStructure of Chelator-GID SR4 bound to Fbp1 substrateEMD-12548other EM volumeStructure of GID SR4EMD-12538other EM volumeStructure of endogenous GID AntEMD-12540other EM volumeStructure of endogenous Chelator-GID AntEMD-12563associated EM volumeSupramolecular assembly module of yeast Chelator-GID SR4 E3 ubiquitin ligase7nsbFULLOVERLAPSupramolecular assembly module of yeast Chelator-GID SR4 comprising Gid1, Gid8 and a homodimer of Gid7Supramolecular assembly module of yeast Chelator-GID SR4 comprising Gid1, Gid8 and a homodimer of Gid70123Generated by focused refinement of Chelator-GID SR4 + Fbp1 mapSaccharomyces cerevisiaeTrichoplusia ni0.33Vacuolar import and degradation protein 30Saccharomyces cerevisiae (strain ATCC 204508 / S288c)ATCC 204508 / S288c0.108287679999999981Trichoplusia niLEVOMSEYMDDVDREFINCLFPSYLLQQPVAYDLWILYLQHRKLFHKLKNTNLINADENPTGVGMGRTKLTALTRKEIWSKLMN
LGVLGTISFEAVNDDYLIQVYKYFYPDVNDFTLRFGVKDSNKNSVRVMKASSDMRKNAQELLEPVLSEREMALNSNTSLE
NDRNDDDDDDDDDDDDDDDDDDDDDESDLESLEGEVDTDTDDNNEGDGSDNHEEGGEEGSRGADADVSSAQQRAERVADP
WIYQRSRSAINIETESRNLWDTSDKNSGLQYYPPDQSPSSSFSSPRVSSGNDKNDNEATNVLSNSGSKKKNSMIPDIYKI
LGYFLPSRWQAQPNNSLQLSQDGITHLQPNPDYHSYMTYERSSASSASTRNRLRTSFENSGKVDFAVTWANKSLPDNKLT
IFYYEIKVLSVTSTESAENSNIVIGYKLVENELMEATTKKSVSRSSVAGSSSSLGGSNNMSSNRVPSTSFTMEGTQRRDY
IYEGGVSAMSLNVDGSINKCQKYGFDLNVFGYCGFDGLITNSTEQSKEYAKPFGRDDVIGCGINFIDGSIFFTKNGIHLG
NAFTDLNDLEFVPYVALRPGNSIKTNFGLNEDFVFDIIGYQDKWKSLAYEHICRGRQMDVSIEEFDSDESEEDETENGPE
ENKSTNVNEDLMDIDQEDGAAGNKDTKKLNDEKDNNLKFLLGEDNRFIDGKLVRPDVNNINNLSVDDGSLPNTLNVMIND
YLIHEGLVDVAKGFLKDLQKDAVNVNGQHSESKDVIRHNERQIMKEERMVKIRQELRYLINKGQISKCINYIDNEIPDLL
KNNLELVFELKLANYLVMIKKSSSKDDDEIENLILKGQELSNEFIYDTKIPQSLRDRFSGQLSNVSALLAYSNPLVEAPK
EISGYLSDEYLQERLFQVSNNTILTFLHKDSECALENVISNTRAMLSTLLEYNAFGSTNSSDPRYYKAINFDEDVLNLGlucose-induced degradation protein 7Saccharomyces cerevisiae (strain ATCC 204508 / S288c)ATCC 204508 / S288c0.0846072972Escherichia coliLEVOMSHTNKIAYVLNNDTEETASPSSVGCFDKKQLTKLLIHTLKELGYDSAANQLLLESGGYQNESNHIQTFFKLIKTGQFHL
INWQIVCSLPLAHSSPLRSEWLQRLLIPTPTPATTSLFDHMLLQLQYLQQLMSSVNSSTCSDAEIATLRNYVEIMILVNR
QIFLEFFHPVTNSASHKGPHTALPVLYLRKILKNFIEIWDSLLVSNDQFLNEENIFNPETTLRELSTYLTNPKLTAQLNL
ERDHLIDAISKYIDPNELVPKGRLLHLLKQAIKYQQSQDIFNIIDPDDDASFSSPPHRINLLQDNFSHDLTVTFQEWKTI
QDTTDEIWFLTFSPNGKYLASATSESSRGYFITVYDVEQDFKIYKTCVSLSQSVLYLMFSPDSRYLVACPFSEDVTIYDM
NATSLPDASATDSFLLYPSTRLSPMDSFKLDTTTYPDDTESSASSSSRPANANSNQSRVWCCDAFHTAERAGWMVVGSPD
REAIVHSLTTKESLFSLKGRTCIALGHDENISGRKSIDPAKVLYKPTSSNGNWQYVEDDETFPRVHDVKISYDDKYVLLM
THQGVIDVYDFSGFPSKEELSKQTVDPKNFLIPRIARLDVGKNMTCISLPLNTTHQGFHRQQISESQHLVLVSLQDNELQ
MWDYKENILIQKYFGQKQQHFIIRSCFAYGNKLVMSGSEDGKIYIWDRIRGNLVSVLSGHSTVMSNSTKPMGKNCNVVAS
NPADKEMFASGGDDGKIKIWKISRNGlucose-induced degradation protein 8Saccharomyces cerevisiae (strain ATCC 204508 / S288c)ATCC 204508 / S288c0.0558033089999999951Trichoplusia niLEVOMTISTLSNETTKSGSCSGQGKNGGKDFTYGKKCFTKEEWKEQVAKYSAMGELYANKTIHYPLKIQPNSSGGSQDEGFATI
QTTPIEPTLPRLLLNYFVSMAYEDSSIRMAKELGFIRNNKDIAVFNDLYKIKERFHIKHLIKLGRINEAMEEINSIFGLE
VLEETFNATGSYTGRTDRQQQQQQQQFDIDGDLHFKLLLLNLIEMIRSHHQQENITKDSNDFILNLIQYSQNKLAIKASS
SVKKMQELELAMTLLLFPLSDSADSGSIKLPKSLQNLYSISLRSKIADLVNEKLLKFIHPRIQFEISNNNSKFPDLLNSD
KKIITQNFTVYNNNLVNGSNGTKITHISSDQPINEKMSSNEVTAAANSVWLNQRDGNVGTGSAATTFHNLENKNYWNQTS
ELLSSSNGKEKGLEFNNYYSSEFPYEPRLTQIMKLWCWCENQLHHNQIGVPRVENSDENLYFQSGWSHPQFEKGGGSGGG
SGGSAWSHPQFEKsingleParticleparticle7.5ETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K3 BIOQUANTUM (6k x 4k)79.21EMD-125573.7FSC 0.143 CUT-OFF48629MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODemd_12563_msk_1.mapemd_12563_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
288
288288
0
00288288288455.04455.04455.0490.090.090.0XYZ-0.00178945841.9438540.00051992440.0170944391.581.581.58emd_12563_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
288
288288
0
00288288288455.04455.04455.0490.090.090.0XYZ-0.050020510.100843748.4395964e-050.0029876471.581.581.58emd_12563_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)