2021-11-24RELPDBePDBePDBe2021-03-082021-11-242021-11-242021-11-24German Research Foundation (DFG)FOR2969GermanyAL amyloid fibril from a lambda 1 light chainKarimi Farsijani SRadamaker LFandrich MRadamaker LKarimi-Farsijani SAndreotti GBaur JNeumann MSchreiner SBerghaus NMotika RHaupt CWalther PSchmidt VHuhn SHegenbart USchonland SOWiese SRead CSchmidt MFandrich MRole of mutations and post-translational modifications in systemic AL amyloidosis studied by cryo-EM.Nat CommunUK1264346434202134741031doi:10.1038/s41467-021-26553-92041-1723EMD-12570associated EM volumeAL amyloid fibril from a lambda 1 light chain7nslFULLOVERLAPAmyloid fibril of an antibody lambda 1 immunoglobulin light chainAmyloid fibril of an antibody lambda 1 immunoglobulin light chain01Extracted fibrils from the explanted heart of a patient suffering from systemic AL amyloidosisHomo sapiensHeartHeart muscleAmyloid lambda1 light chainHumanHeartHeart muscle tissue0.0121223539999999987LEVOQSVLTQPPSVSAAPGQNVTISCSGSSSNIGNNYVSWYQQLPGTAPKLLIYETDKRPSGIPDRFSGSKSGTSATLGITGLQ
TADEADYYCGTWESSLLAGVFGGGTKLTVLGQPKAAPShelicalfilament7.0H2ODistilled waterC-flat-1.2/1.3COPPERCARBONHOLEYGLOW DISCHARGEOTHERETHANE95295FEI VITROBOT MARK IIIblot for 9s before plunging. Sample in pure water, pH not determinedFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.7FEI TITAN KRIOS AUTOGRID HOLDERNITROGEN20GATAN K2 SUMMIT (4k x 4k)COUNTING383837101303240.01Motion-corrected and dose-weighted movie frames4.76311-1.45566C13.1FSC 0.143 CUT-OFFRELION3.043308Gctf1.06CTF was estimated from the non-dose-weighted micrographs43308manual particle picking helical start-end coordinatesInitial model generation in RELION, followed by a 3D classification of particles picked from 28 micrographs to generate rough 3D model which was used as a referenceNOT APPLICABLEOTHERSecondary structure restraints and NCS were applied during refinementREAL-SPACE (WEIGHTED MAP SUM AT ATOM CENTERS)REALemd_12570_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
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00260260260270.4270.4270.490.090.090.0XYZ-0.0259481180.0569598342.1486598e-050.00318610231.041.041.04emd_12570_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)