2021-07-07RELPDBePDBePDBe2021-04-302021-06-232021-06-232021-07-07Swiss National Science Foundation31003A_182341SwitzerlandEuropean Molecular Biology Organization (EMBO)1074-2019Swiss National Science Foundation182880State D0'' of the human mitoribosomal large subunit assembly intermediateLenarcic TJaskolowski MLeibundgut MScaiola ASchoenhut TSaurer MLee RGRackham OFilipovska ABan NLenarcic TJaskolowski MLeibundgut MScaiola ASchonhut TSaurer MLee RGRackham OFilipovska ABan NStepwise maturation of the peptidyl transferase region of human mitoribosomes.Nat CommunUK1236713671202134135320doi:10.1038/s41467-021-23811-82041-1723EMD-12851associated EM volumeState D0'' of the human mitoribosomal large subunit assembly intermediateState D0'' of the human mitoribosomal large subunit assembly intermediate0Homo sapiensHEK 293 EBNAsingleParticleparticle7.620.0HEPES-KOH100.0KCl20.0MgCl21.0DTTQuantifoil R2/2COPPERCARBONCONTINUOUSETHANE-PROPANE100277.15FEI VITROBOT MARK IV4 uL of the sample was blotted for 2-6 sec before plunging. FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300100.02.7GATAN K3 (6k x 4k)60.01cryoSPARC3.13.5FSC 0.143 CUT-OFFcryoSPARC3.132871MAXIMUM LIKELIHOODcryoSPARC3.1MAXIMUM LIKELIHOODcryoSPARC3.1