2022-01-19RELPDBePDBePDBe2021-08-022021-09-292021-09-292022-01-19Swiss National Science FoundationCRSK-3_190414SwitzerlandNovartis FreeNovationSwitzerlandPromedica Siftung1401/MSwitzerlandCryo-EM structure of the MoStoNano fusion proteinBenoit RMPoghosyan ECollu GBierig TKrebs ASEngilberge SVarma NGuixa-Gonzalez RSharpe TDeupi XOlieric VPoghosyan EBenoit RMChimeric single alpha-helical domains as rigid fusion protein connections for protein nanotechnology and structural biology.StructureUK3095106.e7202234587504doi:10.1016/j.str.2021.09.0020969-21262005STRUE6Benoit RMPoghosyan EChimeric single alpha-helical domains as rigid fusion protein connections for protein nanotechnology and structural biologyTo Be Published0353EMD-13292associated EM volumeMoStoNano fusion proteinMoStoNano fusion protein0Azotobacter vinelandiiEscherichia colisingleParticleparticle0.46.5UltrAuFoil R1.2/1.3GOLD200ETHANE100FEI VITROBOT MARK IVThe sample was monodisperse.FEI TITAN KRIOSOTHERBRIGHT FIELDFIELD EMISSION GUN3002.7165000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)COUNTING38383710247262.01The dataset was pre-processed using MotionCor2 with dose weighting for movie alignmentCTFFIND42.9FSC 0.143 CUT-OFFRELION3.0The final 3D reconstruction was calculated with D3 symmetry imposed.emd_13292_msk_1.mapemd_13292_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
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00300300300246.0246.0246.090.090.090.0XYZ-0.0217527360.064082981.3214979e-050.00210649570.820.820.82Unfiltered half map after the refinementemd_13292_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
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00300300300246.0246.0246.090.090.090.0XYZ-0.018919270.0657338651.248984e-050.00210981560.820.820.82Unfiltered half map after the refinement