2021-12-01RELPDBePDBePDBe2021-10-152021-11-242021-11-242021-12-01German Research Foundation (DFG)SFB 1035GermanyPreliminary Single particle cryo-EM reconstruction of a 40-mer assembly of Hsp26 purified from yeast.Peters CWeinkauf SBuchner JMuhlhofer MPeters CKriehuber TKreuzeder MKazman PRodina NReif BHaslbeck MWeinkauf SBuchner JPhosphorylation activates the yeast small heat shock protein Hsp26 by weakening domain contacts in the oligomer ensemble.Nat CommunUK1266976697202134795272doi:10.1038/s41467-021-27036-72041-1723White HEOrlova EVChen SWang LIgnatiou AGowen BStromer TFranzmann TMHaslbeck MBuchner JSaibil HRMultiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26.StructureUK14119712042006doi:10.1016/j.str.2006.05.0210969-21262005STRUE6EMD-12773other EM volumeEMD-12772other EM volumeEMD-12771other EM volumeEMD-12766other EM volumeEMD-13748associated EM volume40mer complex of WT Hsp2640mer complex of WT Hsp2601Saccharomyces cerevisiaeSaccharomyces cerevisiaeJT(DIP) GPD26(A)pJV5170.9552Hsp26Saccharomyces cerevisiaeSaccharomyces cerevisiaeLEVOMSFNSPFFDFFDNINNEVDAFNRLLGEGGLRGYAPRRQLANTPAKDSTGKEVARPNNYAG
ALYDPRDETLDDWFDNDLSLFPSGFGFPRSVAVPVDILDHDNNYELKVVVPGVKSKKDID
IEYHQNKNQILVSGEIPSTLNEESKDKVKVKESSSGKFKRVITLPDYPGVDADNIKADYA
NGVLTLTVPKLKPQKDGKNHVKKIEVSSQESWGNsingleParticleparticle7.4Quantifoil R2/1COPPER400ETHANE-PROPANE100294FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.70.62.8000000000000003105000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGIF Quantum LS20GATAN K2 SUMMIT (4k x 4k)COUNTING11708.0140.01107702ab initio reconstruction with cryosparcC410.0FSC 0.143 CUT-OFFcryoSPARC37763MAXIMUM LIKELIHOODcryoSPARCMAXIMUM LIKELIHOODcryoSPARC