2021-12-08RELPDBePDBePDBe2021-10-202021-12-012021-12-012021-12-08Russian Science Foundation19-74-20055Russian FederationGroEL - PrP complexMoiseenko AVPichkur EBKudryavtseva SSStanishneva-Konovalova TBMamchur AAMoiseenko AVPanina ISYaroshevich IAKudryavtseva SSPichkur EBSokolova OSMuronetz VIStanishneva-Konovalova TBStructural and Computational Study of the GroEL-Prion Protein Complex.Biomedicines9202134829878doi:10.3390/biomedicines91116492227-9059EMD-13762associated EM volumeComplex of GroEL with Prion proteinComplex of GroEL with Prion protein012GroEL11Escherichia coli K-12Prion protein12Ovis ariesEscherichia coliGroELEscherichia coli K-12LEVOAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMMPrion proteinOvis ariesEscherichia coliLEVOSKKRPKPGGGWNTGGSRYPGQGSPGGNRYPPQGGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGGWGQGGSHSQWNKPSKPKTNMKHVAGAAAAGAVVGGLGGYMLGSAMSRPLIHFGNDYEDRYYRENMYRYPNQVYYRPVDRYSNQNNFVHDCVNITVKQHTVTTTTKGENFTETDIKIMERVVEQMCITQYQRESQAYYQRGAsingleParticleparticle7.5Quantifoil R1.2/1.3COPPERGLOW DISCHARGEETHANE100277.5FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3000.05FEI TITAN KRIOS AUTOGRID HOLDERFEI FALCON II (4k x 4k)1621100.01154000CTFFINDInitial model generated in Relion with SGDC14.0FSC 0.143 CUT-OFFRELION3.0.8Final reconstruction was performed on C2-symmetry expanded dataset with the mask encapsulating one of the GroEL rings along with the PrP volume. The other ring density was subjected to particle subtraction.82300MAXIMUM LIKELIHOODRELION3.0.8MAXIMUM LIKELIHOODRELION3.0.86RELION3.0.8The classification performed on C2-symmetry expanded dataset with the mask encapsulating one of the GroEL rings along with the PrP volume. The other ring density was subjected to particle subtraction.