REL
PDBe
PDBe
PDBe
2008-02-05
2008-02-07
2009-03-31
2009-03-31
Human Parvovirus B19 (empty wildtype particle)
Kaufmann B
Chipman PR
Modrow S
Rossmann MG
icosahedral, empty viral particle, B19
Kaufmann B
Chipman PR
Kostyuchenko VA
Modrow S
Rossmann MG
Visualization of the externalized VP2 N termini of infectious human parvovirus B19.
J.VIROL.
82
7306
7312
2008
18508892
doi:10.1128/JVI.00512-08
Human Parvovirus B19 (empty wildtype particle)
Human Parvovirus B19 (empty wildtype particle)
icosahedral, empty viral particle, non-enveloped protein shell
60 VP subunits form icosahedral protein shell
1
3.6
Human parvovirus B19
empty wildtype particles purified from human plasma, no ssDNA genome encapsidated
Human parvovirus B19
Homo sapiens
VERTEBRATES
3.6
260
1
OTHER
SPECIES
false
true
Human Parvovirus B19
singleParticle
particle
1
7.5
25mM Tris-HCl
400 mesh copper
ETHANE
HOMEMADE PLUNGER
Vitrification instrument: guillotine-style plunge freezing device
A small vial of ethane is placed inside a larger liquid nitrogen reservoir. The grid holding a few microliters of the sample is held in place at the bottom of a plunger by the means of fine tweezers. Once the ethane in the vial is completely frozen, it needs to be slightly melted. When the liquid ethane is ready, a piece of filter paper is then pressed against the sample to blot of excess buffer, sufficient to leave a thin layer on the grid. After a predetermined time, the filter paper is removed, and the plunger is allowed to drop into the liquid ethane. Once the grid enters the liquid ethane, the sample is rapidly frozen, and the grid is transferred under liquid nitrogen to a storage box immersed liquid nitrogen for later use in the microscope.
FEI/PHILIPS CM300FEG/T
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
2.0
1.25
3.66
45000.0
47000.0
GATAN LIQUID NITROGEN
98
live FFT at 200K
low dose
KODAK SO-163 FILM
ZEISS SCAI
14
31
22
8.
Eucentric
0
0
manual particle selection
each particle
I
OTHER
11.3
FSC 0.5 CUT-OFF
EMPFT, POR, P3DR
final map includes data to 10.5 Ang resolution (fsc 0.3 cut-off), magnification of final map standardized to a map calculated from B19 VP2 VLP model coordinates (PDB accession no 1S58) resulting in final pixel separation of 2.8165Ang
1959