2020-08-12RELRCSBRCSBRCSB2019-04-262019-05-222019-11-132020-08-12National Institutes of Health/National Institute of General Medical SciencesR01GM083122United StatesNational Institutes of Health/National Institute of General Medical SciencesR37GM030626United StatesNational Institutes of Health/National Institute of General Medical SciencesR01GM112050United StatesNational Institutes of Health/National Institute of General Medical SciencesR35GM122574United StatesThe central pair apparatus focusing on the C1a-e-c supercomplex extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas fap216 mutant axonemeFu GNicastro DFu GZhao LDymek EHou YSong KPhan NShang ZSmith EFWitman GBNicastro DStructural organization of the C1a-e-c supercomplex within the ciliary central apparatus.J.Cell Biol.US21842364251201931672705doi:10.1083/jcb.2019060061540-81402019JCLBA3EMD-20160other EM volumeEMD-20161other EM volumeEMD-20162other EM volumeEMD-20163other EM volumeEMD-20164other EM volumeEMD-20165associated EM volumeEMD-20166other EM volumeThe C1a-e-c supercomplex of central pair apparatus averaged from Chlamydomonas fap216 mutant ciliaThe C1a-e-c supercomplex of central pair apparatus averaged from Chlamydomonas fap216 mutant cilia0Chlamydomonas reinhardtiiciliasubtomogramAveragingcell7.4CARBONHOLEYunspecifiedETHANE298HOMEMADE PLUNGERback-side blotting with No.1 Whatman filter for 1.5-2.5 seconds before plunging. Freshly isolated and demembranated cilia from Chlamydomonas fap216 mutant cellsFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300100.00.20.526000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENVOLTA PHASE PLATEGIF Bioquantum20GATAN K2 SUMMIT (4k x 4k)COUNTING371038381-156.01.571BACK PROJECTION22.0FSC 0.5 CUT-OFFIMOD1380241380MATLABOTHER