2024-03-20RELRCSBRCSBRCSB2019-07-182020-02-052020-02-122024-03-20National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI136680United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM069832United StatesNational Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI146017United StatesStructure of HIV cleaved synaptic complex (CSC) intasome bound with magnesium and INSTI XZ446 (compound 4f)Lyumkis DPassos Dintegrase, intasome, transposition, VIRAL PROTEIN-DNA complexPassos DOLi MJozwik IKZhao XZSantos-Martins DYang RSmith SJJeon YForli SHughes SHBurke Jr TRCraigie RLyumkis DStructural basis for strand-transfer inhibitor binding to HIV intasomes.ScienceUS367810814202032001521doi:10.1126/science.aay80151095-92030038SCIEAS6puzFULLOVERLAPAssembly of HIV integrase and viral DNAAssembly of HIV integrase and viral DNA0123hiv0.4Chimeric Sso7d and HIV-1 integraseHuman immunodeficiency virus 10.0423212584Escherichia coliLEVOMGSSHHHHHHSSGLVPRGSHMATVKFKYKGEEKEVDISKIKKVWRVGKMISFTYDEGGGKTGRGAVSEKDAPKELLQMLE
KQKKGGGGGGGGGGGFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVASCDKCQLKGEAMHGQVDCSPGIWQLDC
THLEGKVILVAVHVASGYIEAEVIPAETGQETAYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNP
QSQGVIESMNKELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQKQITKIQNFRV
YYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQMAGDDCVASRQDEDP39476Q76353viral DNA non-transferred strandHuman immunodeficiency virus 10.0081882709999999991(DA)(DC)(DT)(DG)(DC)(DT)(DA)(DG)(DA)(DG)(DA)(DT)(DT)(DT)(DT)(DC)(DC)(DC)(DG)(DC)
(DC)(DC)(DA)(DC)(DG)(DC)(DT)DNAviral DNA transferred strandHuman immunodeficiency virus 10.00777302299999999961(DA)(DG)(DC)(DG)(DT)(DG)(DG)(DG)(DC)(DG)(DG)(DG)(DA)(DA)(DA)(DA)(DT)(DC)(DT)(DC)
(DT)(DA)(DG)(DC)(DA)DNAMAGNESIUM ION2.4305e-052MGZINC ION6.5409e-052ZN4-azanyl-N-[[2,4-bis(fluoranyl)phenyl]methyl]-1-oxidanyl-2-oxidanylidene-6-[2-(phenylsulfonyl)ethyl]-1,8-naphthyridine-3-carboxamide0.0005145011XXJwater1.8015e-05170HOHsingleParticleparticle0.26.220.0Bis-Tris550.0NaClsodium chloride5.0MgClmagnesium chloride0.5TCEP5.0glycerolUltrAuFoilGOLD400PLASMA CLEANINGETHANE80HOMEMADE PLUNGERFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30070.02.71.53.037000.063291.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)COUNTING383837104-80207242.01412354C2FOURIER SPACE2.8FSC 0.143 CUT-OFFcisTEM1.0 beta143698PROJECTION MATCHINGcisTEM1.0PROJECTION MATCHINGcisTEM1.0 beta2cisTEM1.0 beta3L3UPDBexperimental model1K6YPDBexperimental model5U1CPDBexperimental modelFLEXIBLE FITCorrelation coefficientREALemd_20485_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
384
384384
0
00384384384303.36002303.36002303.3600290.090.090.0XYZ-7.52958717.786429999999999-0.0056326820.347487840.790.790.79Structure of HIV cleaved synaptic complex intasome bound to 4f. Full mapemd_20485_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
384
384384
0
00384384384303.36002303.36002303.3600290.090.090.0XYZ0.00.29817710.115212420.117730870.790.790.79local resolutionemd_20485_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
384
384384
0
00384384384303.36002303.36002303.3600290.090.090.0XYZ-12.11713999999999920.171672999999998-0.0277765650.76932990.790.790.79half map 1emd_20485_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)