2020-10-14
REL
RCSB
RCSB
RCSB
2019-08-26
2019-09-11
2020-10-14
2020-10-14
Canadian Institutes of Health Research
Operating grant
Canada
PmtCD peptide toxin ABC exporter in nucleotide free (apo) conformation
Zeytuni N
Strynadka NCJ
Yu Z
Chou HT
Zeytuni N
Dickey SW
Hu J
Chou HT
Worrall LJ
Alexander JAN
Carlson ML
Nosella M
Duong F
Yu Z
Otto M
Strynadka NCJ
Structural insight into the Staphylococcus aureus ATP-driven exporter of virulent peptide toxins
Sci Adv
US
6
eabb8219
2020
32998902
doi:10.1126/sciadv.abb8219
2375-2548
EMD-20639
other EM volume
ATP bound conformation
EMD-20640
associated EM volume
PmtCD
PmtCD
0
1
2
PmtCD ABC exporter
Staphylococcus aureus
0.12
PmtD
Staphylococcus aureus
DEXTRO
MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDNPKHIIVYGSVFAAAKW
LLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTISIILISILFALIHYVILI
VVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLASQKTAMIFSLGVFLVLIVPF
IKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDFSNWQWVISLGSIVIFFILNILY
VAKKDI
PmtC
Staphylococcus aureus
DEXTRO
MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMKVMNGNIIKFDGKVDID
NADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDKIIDAFGMRPYIKKKVKKY
SMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLTTIKSLVNELDMRILISSHKL
EDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVDHKDFDRTEKYLAEHFQLQNVDK
ADGHLMINAQKNYQVILKALSELDIYPKYIETRKSSLRDTYFNINQRGDK
singleParticle
particle
1
7.4
20.0
C4H11NO3
Tris
150.0
NaCl
sodium chloride
Solution made fresh from concentrated stocks and filtered
CARBON
HOLEY
unspecified
ETHANE
100
277
FEI VITROBOT MARK I
FEI TITAN KRIOS
FLOOD BEAM
BRIGHT FIELD
FIELD EMISSION GUN
300
GATAN K2 SUMMIT (4k x 4k)
SUPER-RESOLUTION
1.45
1
440420
CTFFIND
4
C1
8.14
FSC 0.143 CUT-OFF
cryoSPARC
1
76140
MAXIMUM LIKELIHOOD
MAXIMUM LIKELIHOOD
AB INITIO MODEL