2019-12-11RELRCSBRCSBRCSB2019-09-122019-12-042019-12-042019-12-11National Institutes of Health/National Institute Of Allergy and Infectious DiseasesR01AI127401United StatesIn vivo structure of the Legionella type II secretion system by electron cryotomography (aligning the IM-complex).Ghosal DJensen GJGhosal DKim KWZheng HKaplan MTruchan HKLopez AEMcIntire IEVogel JPCianciotto NPJensen GJIn vivo structure of the Legionella type II secretion system by electron cryotomography.Nat MicrobiolUK421012108201931754273doi:10.1038/s41564-019-0603-62058-5276EMD-20712associated EM volumeSubtomogram average of the Legionella pneumophila type II secretion system (aligning the IM-complex).Subtomogram average of the Legionella pneumophila type II secretion system (aligning the IM-complex).0We used electron cryotomography and subtomogram averaging to visualize L. pneumophila type II secretion system complex directly in intact, frozen-hydrated bacterial cellsLegionella pneumophilaLp02subtomogramAveragingcell6.9ETHANE-PROPANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)1.21C250.0FSC 0.143 CUT-OFFIMOD4401993440PEETOTHER