2024-03-20RELRCSBRCSBRCSB2019-09-262019-10-092019-10-092024-03-20National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)1 ZIA NS003165United StatesKatanin hexamer in the spiral conformation in complex with substrateZehr EARoll-Mecak Akatanin, microtubule-severing, MEI-1, microtubule cytoskeleton, MOTOR PROTEINZehr EASzyk ASzczesna ERoll-Mecak AKatanin Grips the beta-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules.Dev.CellUS52118131.e6202031735665doi:10.1016/j.devcel.2019.10.0101534-58076ugdFULLOVERLAPHexameric complex of C.elegans katanin bound to polyglutamate peptideHexameric complex of C.elegans katanin bound to polyglutamate peptide012Caenorhabditis elegans0.312Meiotic spindle formation protein mei-1Caenorhabditis elegans0.0535763366Escherichia coli BL21(DE3)LEVOGSFTMASMTGGQQMGRGSMNGDVQSVIRGYLERAQVAKTMSDAGRWNEAGDLLRQLMTDVKSCKISASNRDEHDARNTFL
RALEANLKLVQQNVRDEDDLHEAMTRQSGSPEPPADPDVWSKPSPPLPSSSKFGATKKGVGAAGPRPREISKSTSSMSTN
PADVKPANPTQGILPQNSAGDSFDASAYDAYIVQAVRGTMATNTENTMSLDDIIGMHDVKQVLHEAVTLPLLVPEFFQGL
RSPWKAMVLAGPPGTGKTLIARAIASESSSTFFTVSSTDLSSKWRGDSEKIVRLLFELARFYAPSIIFIDQIDTLGGQRG
NSGEHEASRRVKSEFLVQMDGSQNKFDSRRVFVLAATNIPWELDEALRRRFEKRIFIPLPDIDARKKLIEKSMEGTPKSD
EINYDDLAARTEGFSGADVVSLCRTAAINVLRRYDTKSLRGGELTAAMESLKAELVRNIDFEAALQAVSPSAGPDTMLKC
KEWCDSFGAMP348085.6.1.1Polyglutamate peptidesynthetic construct0.00182560899999999971LEVOEEEEEEEEEEEEEEADENOSINE-5'-TRIPHOSPHATE0.0005071816ATPMAGNESIUM ION2.4305e-055MGsingleParticleparticle17.520.0C8H18N2O4SHEPES300.0KClPotassium chloride10.0MgCl2Magnesium dichloride2.0C9H15O6PTCEP1.0C10H16N5O13P3ATPC-flat-2/1GOLD400CARBONHOLEYPLASMA CLEANINGOTHERETHANE90279.15LEICA EM GPLoad 5 ul sample, wait 10 sec, blot 4.5 sec. FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30070.02.71.01.02.80000000000000032.8000000000000003130000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGEN93.0103.0GIF Quantum LS20GATAN K2 SUMMIT (4k x 4k)SUPER-RESOLUTION742076761-504591110.073.01Frames were aligned, dose weighted and summed using MotionCor21876223Particles were automatically pickedEMD-8794Low-pass filtered to 35 A1C1FOURIER SPACE3.5FSC 0.143 CUT-OFFRELION3.040102MAXIMUM LIKELIHOODRELION3.0MAXIMUM LIKELIHOODRELION3.010122136.0RELION3.05WC0PDBexperimental modelFLEXIBLE FITCorrelation coefficientREAL