2024-03-06RELRCSBRCSBRCSB2019-11-052019-12-042019-12-042024-03-06National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM43949United StatesSingle particle cryo-EM structure of KvAPTao XMacKinnon Rvoltage-gated potassium channel, non-domain-swapped, TRANSPORT PROTEINTao XMacKinnon RCryo-EM structure of the KvAP channel reveals a non-domain-swapped voltage sensor topology.ElifeUS8201931755864doi:10.7554/eLife.521642050-084X6uwmFULLOVERLAPKvAP-6E1 Fab complexKvAP-6E1 Fab complex010.2KvAP11KvAP tetramerAeropyrum pernix0.136E1 Fab1unidentifiedVoltage-gated potassium channelAeropyrum pernix0.0316492074Escherichia coli BL21(DE3)LEVOMAGGRVRNIGDVMEHPLVELGVSYAALLSVIVVVVEYTMQLSGEYLVRLYLVDLILVIILWADYAYRAYKSGDPAGYVKK
TLYEIPALVPAGLLALIEGHLAGLGLFRLVRLLRFLRILLIISRGSKFLSAIADAADKIRFYHLFGAVMLTVLYGAFAIY
IVEYPDPNSSIKSVFDALWWAVVTATTVGYGDVVPATPIGKVIGIAVMLTGISALTLLIGTVSNMFQKILVGEPEPSSSP
AKLAEMVSSMSEEEFEEFVRTLKNLRRLENSMKLVPRGSRSHHHHHHQ9YDF8singleParticleparticle68.0150.0KClpotassium chloride20.0C4H11NO3Tris0.5C56H92O29DigitoninQuantifoil R1.2/1.3GOLD400CARBONHOLEYGLOW DISCHARGEAIRETHANE100295FEI VITROBOT MARK IVBlot for 4 seconds before plunging.. KvAP in complex with 6E1 FabFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30070.02.70.82.229000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)SUPER-RESOLUTION1-502800010.075.0118000002C45.9FSC 0.143 CUT-OFFFREALIGN9.1167000PROJECTION MATCHINGcryoSPARC2.9.0PROJECTION MATCHINGFREALIGN9.11332000.0RELION3.0.81ORSPDBexperimental model1ORQPDBexperimental modelRIGID BODY FITREAL