2024-03-06RELRCSBRCSBRCSB2020-02-052020-07-292020-07-292024-03-06Howard Hughes Medical Institute (HHMI)United StatesStructure of a bacterial Atm1-family ABC exporterFan CRees DCABC transporter, membrane protein, TRANSPORT PROTEINFan CKaiser JTRees DCA structural framework for unidirectional transport by a bacterial ABC exporter.Proc.Natl.Acad.Sci.USAUS1171922819236202032703810doi:10.1073/pnas.20065261171091-64900040PNASA66vquFULLOVERLAPhomodimeric Atm1-type heavy metal transporterhomodimeric Atm1-type heavy metal transporter01Novosphingobium aromaticivorans DSM 124440.133ATM1-type heavy metal exporterNovosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F1990.0677716022Escherichia coli BL21(DE3)LEVOMPPETATNPKDARHDGWQTLKRFLPYLWPADNAVLRRRVVGAILMVLLGKATTLALPFAYKKAVDAMTLGGGAQPALTVA
LAFVLAYALGRFSGVLFDNLRNIVFERVGQDATRHLAENVFARLHKLSLRFHLARRTGEVTKVIERGTKSIDTMLYFLLF
NIAPTVIELTAVIVIFWLNFGLGLVTATILAVIAYVWTTRTITEWRTHLREKMNRLDGQALARAVDSLLNYETVKYFGAE
SREEARYASAARAYADAAVKSENSLGLLNIAQALIVNLLMAGAMAWTVYGWSQGKLTVGDLVFVNTYLTQLFRPLDMLGM
VYRTIRQGLIDMAEMFRLIDTHIEVADVPNAPALVVNRPSVTFDNVVFGYDRDREILHGLSFEVAAGSRVAIVGPSGAGK
STIARLLFRFYDPWEGRILIDGQDIAHVTQTSLRAALGIVPQDSVLFNDTIGYNIAYGRDGASRAEVDAAAKGAAIADFI
ARLPQGYDTEVGERGLKLSGGEKQRVAIARTLVKNPPILLFDEATSALDTRTEQDILSTMRAVASHRTTISIAHRLSTIA
DSDTILVLDQGRLAEQGSHLDLLRRDGLYAEMWARQAAESAEVSEAAEHHHHHHQ2G506singleParticleparticle47.5100.0NaClSodium Chloride20.0TrisTrisQuantifoil R1.2/1.3GOLD200GLOW DISCHARGEETHANE100298FEI VITROBOT MARK IVThe sample was reconstituted with MSP1D1 nanodiscs and POPC.FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300100.02.71.03.5165000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K2 SUMMIT (4k x 4k)COUNTING36.0111454444MRNC23.88FSC 0.143 CUT-OFFcryoSPARC102076MAXIMUM LIKELIHOODcryoSPARCMAXIMUM LIKELIHOODcryoSPARC4MRNAPDBexperimental modelRIGID BODY FITREAL23.0emd_21357_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
400
400400
0
00400400400333.6333.6333.690.090.090.0XYZ-0.204053070.445026460.00039006430.011910520.8340.8340.834emd_21357_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
400
400400
0
00400400400333.6333.6333.690.090.090.0XYZ-0.687450470.61565470.00041334350.072623440.8340.8340.834emd_21357_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)