2021-04-14RELRCSBRCSBRCSB2020-02-172020-02-262020-02-262021-04-14National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI123498United StatesNational Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI127521United StatesCryo-EM structure of porcine epidemic diarrhea virus (PEDV) spike proteinKirchdoerfer RNWard ABKirchdoerfer RNBhandari MMartini OSewall LMBangaru SYoon KJWard ABStructure and immune recognition of the porcine epidemic diarrhea virus spike protein.StructureUK29385392.e5202133378641doi:10.1016/j.str.2020.12.0030969-21262005STRUE6EMD-21391associated EM volumeCryo-EM structure of porcine epidemic diarrhea virus (PEDV) spike protein6vv5FULLOVERLAPPorcine epidemic diarrhea virus spike proteinPorcine epidemic diarrhea virus spike protein01Recombinantly expressed in insect cells using a baculovirus vector.Porcine epidemic diarrhea virus13-019349Spodoptera frugiperdaSf90.6Spike glycoproteinPorcine epidemic diarrhea virus0.148463655999999973Spodoptera frugiperdaLEVOLPQDVTRCSANTNFRRFFSKFNVQAPAVVVLGGYLPIGENQGVNSTWYCAGQHPTASGVHGIFVSHIRGGHGFEIGISQE
PFDPSGYQLYLHKATNGNTNATARLRICQFPSIKTLGPTANNDVTTGRNCLFNKAIPAHMSEHSVVGITWDNDRVTVFSD
KIYYFYFKNDWSRVATKCYNSGGCAMQYVYEPTYYMLNVTSAGEDGISYQPCTANCIGYAANVFATEPNGHIPEGFSFNN
WFLLSNDSTLVHGKVVSNQPLLVNCLLAIPKIYGLGQFFSFNQTIDGVCNGAAVQRAPEALRFNINDISVILAEGSIVLH
TALGTNFSFVCSNSSNPHLATFAIPLGATQVPYYCFLKVDTYNSTVYKFLAVLPPTVREIVITKYGDVYVNGFGYLHLGL
LDAVTINFTGHGTDDDVSGFWTIASTNFVDALIEVQGTAIQRILYCDDPVSQLKCSQVAFDLDDGFYTISSRNLLSHEQP
ISFVTLPSFNDHSFVNITVSASFGGHSGANLIASDTTINGFSSFCVDTRQFTISLFYNVTNSYGYVSKSQDSNCPFTLQS
VNDYLSFSKFCVSTSLLASACTIDLFGYPEFGSGVKFTSLYFQFTKGELITGTPKPFEGVTDVSFMTLDVCTKYTIYGFK
GEGIITLTNSSFLAGVYYTSDSGQLLAFKNVTSGAVYSVTPCSFSEQAAYVDDDIVGVISSLSSSTFNSTRELPGFFYHS
NDGSNCTEPVLVYSNIGVCKSGSIGYVPSQSGQVKIAPTVTGNISIPTNFSMSIRTEYLQLYNTPVSVDCATYVCNGNSR
CKQLLTQYTAACKTIESALQLSARLESVEVNSMLTISDEALQLATISSFNGDGYNFTNVLGVSVYDPASGRVVQKRSFIE
DLLFNKVVTNGLGTVDEDYKRCSNGRSVADLVCAQYYSGVMVLPGVVDAEKLHMYSASLIGGMVLGGFTSAAALPFSYAV
QARLNYLALQTDVLQRNQQLLAESFNSAIGNITSAFESVKEAISQTSKGLNTVAHALTKVQEVVNSQGAALTQLTVQLQH
NFQAISSSIDDIYSRLDILSADAQVDRLITGRLSALNAFVAQTLTKYTEVQASRKLAQQKVNECVKSQSQRYGFCGGDGE
HIFSLVQAAPQGLLFLHTVLVPSDFVDVIAIAGLCVNDEIALTLREPGLVLFTHELQNHTATEYFVSSRRMFEPRKPTVS
DFVQIESCVVTYVNLTRDQLPDVIPDYIDVNKTLDEILASLPNRTGPSLPLDVFNATYLNLTGEIADLEQRSESLRNTTE
ELQSLIYNINNTLVDLEWLNRVETGSGYIPEAPRDGQAYVRKDGEWVLLSTFLENLYFQGGHHHHHHAWSHPQFEK2-acetamido-2-deoxy-beta-D-glucopyranose0.00022120815NAGPALMITOLEIC ACID0.000254407999999999976PAMsingleParticleparticle1.37.425.0Tris150.0sodium chlorideUltrAuFoilGOLD300HOLEYPLASMA CLEANINGOTHERETHANE100277FEI VITROBOT MARK IVFEI TECNAI ARCTICAFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN2000.61.4000000000000001FEI TITAN KRIOS AUTOGRID HOLDERNITROGEN100.0GATAN K2 SUMMIT (4k x 4k)COUNTING1-64112.854.9119436GctfSGD initial model generation1C33.5FSC 0.143 CUT-OFFRELION3.019436MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODRELION3.05SZSAOTHERREALemd_21391_msk_1.mapemd_21391_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
280
280280
0
00280280280322.0322.0322.090.090.090.0XYZ-0.031371550.0722750657.260421e-050.00363588151.151.151.15Second half mapemd_21391_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)