2020-09-02RELRCSBRCSBRCSB2020-02-212020-03-252020-09-022020-09-02National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01GM130051-01United StatesSingle Particle Cryo-EM Structure of the Natively Isolated Sec61, TMCO1, Nicalin, TMEM147, and CCDC47 Containing Ribosome-Translocon ComplexMcGilvray PTAnghel SASundaram ATrnka MJZhong FHu HBurlingame ALKeenan RJMcGilvray PTAnghel SASundaram AZhong FTrnka MJFuller JRHu HBurlingame ALKeenan RJAn ER translocon for multi-pass membrane protein biogenesis.ElifeUS9202032820719doi:10.7554/eLife.568892050-084XEMD-21427associated EM volumeEMD-21426other EM volumeEMD-21435other EM volumeNative complex of a translating ribosome and a novel translocon containing the Sec61 complex, TMEM147, NOMO, Nicalin, CCDC47, and TMCO1.Native complex of a translating ribosome and a novel translocon containing the Sec61 complex, TMEM147, NOMO, Nicalin, CCDC47, and TMCO1.0Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum0.05660S Subunit160S ribosomal protein L9
60S ribosomal protein L8
60S ribosomal protein L7a
60S ribosomal protein L7
60S ribosomal protein L6
60S ribosomal protein L5
60S ribosomal protein L4
60S ribosomal protein L39
60S ribosomal protein L38
60S ribosomal protein L37a
60S ribosomal protein L36a
60S ribosomal protein L36
60S ribosomal protein L35a
60S ribosomal protein L35
60S ribosomal protein L34
60S ribosomal protein L32
60S ribosomal protein L31
60S ribosomal protein L30
60S ribosomal protein L3
60S ribosomal protein L28
60S ribosomal protein L27a
60S ribosomal protein L27
60S ribosomal protein L26
60S ribosomal protein L24
60S ribosomal protein L23a
60S ribosomal protein L23
60S ribosomal protein L22-like 1
60S ribosomal protein L22
60S ribosomal protein L21
60S ribosomal protein L19
60S ribosomal protein L18a
60S ribosomal protein L18
60S ribosomal protein L17
60S ribosomal protein L15
60S ribosomal protein L14
60S ribosomal protein L13a
60S ribosomal protein L13
60S ribosomal protein L12
60S ribosomal protein L11
60S ribosomal protein L10a
60S ribosomal protein L10
60S ribosomal export protein NMD3
60S acidic ribosomal protein P2
60S acidic ribosomal protein P1
60S acidic ribosomal protein P0-like
60S acidic ribosomal protein P0
Ubiquitin-60S ribosomal protein L40Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum2.4240S Subunit140S ribosomal protein SA
40S ribosomal protein S9
40S ribosomal protein S8
40S ribosomal protein S7
40S ribosomal protein S6
40S ribosomal protein S5
40S ribosomal protein S4, X isoform
40S ribosomal protein S3a
40S ribosomal protein S30
40S ribosomal protein S3
40S ribosomal protein S29
40S ribosomal protein S28
40S ribosomal protein S27-like
40S ribosomal protein S27
40S ribosomal protein S26
40S ribosomal protein S25
40S ribosomal protein S24
40S ribosomal protein S23
40S ribosomal protein S21
40S ribosomal protein S20
40S ribosomal protein S2
40S ribosomal protein S19
40S ribosomal protein S18
40S ribosomal protein S17
40S ribosomal protein S16
40S ribosomal protein S15a
40S ribosomal protein S15
40S ribosomal protein S14
40S ribosomal protein S13
40S ribosomal protein S12
40S ribosomal protein S11
40S ribosomal protein S10
Ubiquitin-40S ribosomal protein S27aHomo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum1.12Translocon1Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic ReticulumSec61 Complex4Sec61alpha, beta, and gammaHomo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum0.07TMCO14Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic ReticulumNOMO-Nicalin-TMEM147 Complex4Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic ReticulumCCDC474Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S acidic ribosomal protein P02Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S acidic ribosomal protein P12Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S acidic ribosomal protein P22Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L102Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L10a2Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L112Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L122Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L132Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L13a2Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L142Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L152Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L172Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L182Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L18a2Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L192Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L212Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L222Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L232Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L23a2Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L242Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L262Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L272Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L27a2Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L282Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L32Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L302Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L312Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L322Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L342Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L352Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L35a2Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L362Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L36a2Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L37a2Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L382Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L392Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L42Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L52Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L62Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L72Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L7a2Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L82Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum60S ribosomal protein L92Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S103Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S113Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S123Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S133Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S143Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S153Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S15a3Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S163Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S173Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S183Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S193Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S23Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S213Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S233Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S243Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S253Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S263Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S273Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S27-like3Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S283Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S293Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S33Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S303Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S3a3Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S4, X isoform3Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S53Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S63Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S73Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S83Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein S93Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic Reticulum40S ribosomal protein SA3Homo sapiensKidneyHEK293Endoplasmic ReticulumEndoplasmic ReticulumsingleParticleparticle0.47.4150.0C2H3KO2potassium acetate50.0C8H18N2O4SHEPES, pH 7.45.0MgCl2magnesium chloride0.25C56H92O29digitoninC-flat-1.2/1.3COPPER200CARBONCONTINUOUS2.0GLOW DISCHARGEETHANE100298FEI VITROBOT MARK IVTwo filter papers were added to each arm, 2.5 microliters of sample were added to grids, and 0.5 second of drain time was allowed before vitrification.. Sample was well-dispersed on a thin (~2nm) carbon film.FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K3 (6k x 4k)COUNTING155623.850.011049128Gctf0.5EMD-5592EMD-5592, Human ribosome low pass-filtered to 50 Angstrom resolutionC13.4FSC 0.143 CUT-OFFRELION3.182684MAXIMUM LIKELIHOODRELION3.1MAXIMUM LIKELIHOODRELION3.19RELION3.1emd_21427_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
500
500500
0
0500500500500680.0680.0680.090.090.090.0XYZ-0.023059750.061633690.000088434940.00273132741.361.361.36emd_21427_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)