2021-03-24RELRCSBRCSBRCSB2020-02-222020-03-252020-03-252021-03-24National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM098672United StatesNational Science Foundation (NSF, United States)1453847United StatesNational Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)F32GM119322United StatesBurrH bound to DNA Origami GoniometerAksel TYu ZCheng YDouglas SMAksel TYu ZCheng YDouglas SMMolecular goniometers for single-particle cryo-electron microscopy of DNA-binding proteins.Nat.Biotechnol.US39378386202133077960doi:10.1038/s41587-020-0716-81087-01562119NABIF9Aksel TYu ZCheng YDouglas SMMolecular goniometers for single-particle cryo-EM of DNA-binding proteinsBiorxivUS2020doi:10.1101/2020.02.27.968883EMD-21443associated EM volumeBurrH bound to DNABurrH bound to DNA01Paraburkholderia rhizoxinicaEscherichia coli0.081BurrHDEXTROMGSTAFVDQDKQMANRLNLSPLERSKIEKQYGGATTLAFISNKQNELAQILSRADILKIASYDCAAHALQAVLDCGPMLG
KRGFSQSDIVKIAGNIGGAQALQAVLDLESMLGKRGFSRDDIAKMAGNIGGAQTLQAVLDLESAFRERGFSQADIVKIAG
NNGGAQALYSVLDVEPTLGKRGFSRADIVKIAGNTGGAQALHTVLDLEPALGKRGFSRIDIVKIAANNGGAQALHAVLDL
GPTLRECGFSQATIAKIAGNIGGAQALQMVLDLGPALGKRGFSQATIAKIAGNIGGAQALQTVLDLEPALCERGFSQATI
AKMAGNNGGAQALQTVLDLEPALRKRDFRQADIIKIAGNDGGAQALQAVIEHGPTLRQHGFNLADIVKMAGNIGGAQALQ
AVLDLKPVLDEHGFSQPDIVKMAGNIGGAQALQAVLSLGPALRERGFSQPDIVKIAGNTGGAQALQAVLDLELTLVEHGF
SQPDIVRITGNRGGAQALQAVLALELTLRERGFSQPDIVKIAGNSGGAQALQAVLDLELTFRERGFSQADIVKIAGNDGG
TQALHAVLDLERMLGERGFSRADIVNVAGNNGGAQALKAVLEHEATLNERGFSRADIVKIAGNGGGAQALKAVLEHEATL
DERGFSRADIVRIAGNGGGAQALKAVLEHGPTLNERGFNLTDIVEMAANSGGAQALKAVLEHGPTLRQRGLSLIDIVEIA
SNGGAQALKAVLKYGPVLMQAGRSNEEIVHVAARRGGAGRIRKMVAPLLERQWGSGHHHHHHsingleParticleparticle0.18.0Quantifoil R1.2/1.3GOLD300GRAPHENE OXIDECONTINUOUSETHANE100293FEI VITROBOT MARK IVDNA Origami goniometer complexed with BurrH. Total concentration comprises the concentrations of both.FEI TALOS ARCTICAFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN2005.020.022000.0GATAN K3 (6k x 4k)56.01Gctf1.061C16.5FSC 0.143 CUT-OFFRELION3.068482OTHERIn house written python package that can be installed via pip.A priori angles for tilt and rot angles are assigned from DNA Origami Goniometer barcode information. Psi angles are assigned from 2D classifications that are aligned to a common reference.MAXIMUM LIKELIHOODRELION3.0572816.0RELION3.04CJARIGID BODY FITRMSDREALemd_21443_msk_1.mapemd_21443_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
128
128128
0
00128128128232.96232.96232.9690.090.090.0XYZ-0.073779770.13713303-3.235203e-050.00621036071.821.821.82The first half map for BurrH bound to DNA Goniometer tilt DNAemd_21443_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
128
128128
0
00128128128232.96232.96232.9690.090.090.0XYZ-0.075504840.144544870.00021271340.00621387461.821.821.82The second half map for BurrH bound to DNA Goniometer tilt DNA