2024-03-06RELRCSBRCSBRCSB2020-03-082020-04-222020-04-222024-03-06National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM097014United StatesStructure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1)Neupane RPisareva VCrPV 5'-UTR IRES, Internal ribosome entry site, RIBOSOME, Ribosome-TRANSLATION complexNeupane RPisareva VPRodriguez CFPisarev AVFernandez ISA complex IRES at the 5'-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediate.ElifeUS9202032286223doi:10.7554/eLife.545752050-084X6w2sFULLOVERLAPStructure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1)Structure of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit in the open state (Class 1)012345678910111213141516171819202122232425262728293031323334353637383940414243Oryctolagus cuniculus18S rRNAOryctolagus cuniculus0.5477330621UACCUGGUUGAUCCUGCCAGUAGCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUACGCACGGCCGGUACAG
UGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUGGUUCCUUUGGUCGCUCGACUUGGAUAACUGUGGUAAUUCUAGAGCUA
AUACAUGCCGACGGGCGCUGACCCCCUUCGCGGGGGGGAUGCGUGCAUUUAUCAGUGGUGACUCUAGAUAACCUCGGGCC
GAUCGCACGCCCGGCGGCGACGACCCAUUCGAACGUCUGCCCUAUCAACUUUCGAUGGUAGUCGCCGUGCCUACCAUGGU
GACCACGGGUGACGGGGAAUCAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCA
GGCGCGCAAAUUACCCACUCCCGACCCGGGGAGGUAGUGACGAAAAAUAACAAUACAGGACUCUUUCGAGGCCCUGUAAU
UGGAAUGAGUCCACUUUAAAUCCUUUAACGAGGAUCCAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGC
UCCAAUAGCGUAUAUUAAAGUUGCUGCAGUUAAAAAGCUCGUAGUUGGAUCUUGGGAGCGGCCGUCCCCUGCUCGGCGCC
GGCCCGAAGCGUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCCGCCUGGAUACCGCAGCUAGGAAUAAUGGAAUAG
GACCGCGGUUCUAUUUUGUUGGUUUUCGGAACUGAGGCCAUGAUUAAGAGGGACGGCCGGGGGCAUUCGUAUUGCGCCGC
UAGAGGUGAAAUUCUUGGACCGGCGCAAGACGGACCAGAGCGAAAGCAUUUGCCAAGAAUGUUUUCAUUAAUCAAGAACG
AAAGUCGGAGGUUCGAAGACGAUCAGAUACCGUCGUAGUUCCGACCAUAAACGAUGCCGACCGGCGAUGCGGCGGCGUUA
UUCCCAUGACCCGCCGGGCAGCUUCCGGGAAACCAAAGUCUUUGGGUUCCGGGGGGAGUAUGGUUGCAAAGCUGAAACUU
AAAGGAAUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGAAACCUCACCCGGCCC
GGACACGGACAGGAUUGACAGAUUGAUAGCUCUUUCUCGAUUCCGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGA
GCGAUUUGUCUGGUUAAUUCCGAUAACGAACGAGACUCUGGCAUGCUAACUAGUUACGCGACCCCGGUCGGCGUAACUUC
UUAGAGGGACAAGUGGCGUUCAGCCACCCGAGAUUGAGCAAUAACAGGUCUGUGAUGCCCUUAGAUGUCCGGGGCUGCAC
GCGCGCUACACUGACUGGCUCAGCGUGUGCCUACCCUACGCCGGCAGGCGCGGGUAACCCGUUGAACCCCAUUCGUGAUG
GGGAUCGGGGAUUGCAAUUAUUCCCCAUGAACGAGGAAUUCCCAGUAAGUGCGGGUCAUAAGCUUGCGUUGAUUAAGUCC
CUGCCCUUUGUACACACCGCCCGUCGCUACUACCGAUUGGAUGGUUUAGUGAGGCCCUCGGAUCGGCCCCGCCGGGGUGC
CCUGGCGGAGCGCUGAGAAGACGGUCGAACUUGACUAUCUAGAGGAAGUAAAAGUCGUAACAAGGUUUCCGUAGGUGAAC
CUGCGGAAGGAUCAUUACrPV 5'-UTR IRESCricket paralysis virus0.121094952999999991UUCGAUACCAAGAGCUGGUGUCUGAGGGAUUACGACUCGCGUGGUCGGACAGCACAUGUUAGUCAUGUAUUAUGGUGUUU
CCCAACACGUAUCUUUGCUCUAGUAUUGAACUUUAGUGAUGAGUGUACUUGGCAUGUACACCUGAUUAGUUAGCCCUUGU
GGAUACGCCACUGCCGCUCAGGUACAGUAGGACAUACCCCAAAUAUUGGGUUUUUUUUUUAGAGAAGAAAAUUAGUGGCG
AAACGUACGGUUUAUGUCAAAGGAGAAGGUGAUUUUAAUUGCACCUGAAGCAACCCUAGGUCUUCCCUUUGAUGUGAGCU
AAUAAGGAGUAACCGAUUCUUACAAUGUGAUCAUGUCUUUUCAACAAACAAAGGAUCAF218039.1uS2Oryctolagus cuniculus0.0330220551LEVOMSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSR
NTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQTAFREPRLLVVTDPQADHQPLTEASYVNLPTIALCNTDSPLRYVDIA
IPCNNKGAHSVGLMWWMLAQEVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPEPEFTA
TQPEVADWSEGMQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWMGTTTEWSG1TWL4eS1Oryctolagus cuniculus0.0300020611LEVOMAVGKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVA
FRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQ
VRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGK
ATGDETGAKVERADGYEPPVQESVG1SS70uS5Oryctolagus cuniculus0.0275292381LEVOMADDAGAAGGPGGPGGPGNKEWLPVTKLGRLVKDMKIKSLEEIYLFSLPIKESEIIDFFLGAALKDEVLKIMPVQKQTRA
GQRTRFKAFVAIGDYNGHVGLGLKCSKEVATAIRGAIILAKLSIVPVRRGYWGNKIGKPHTVPCKVTGRCGSVLVRLIPA
PRGTGIVSAPVPKKLLLMAGIDDCYTSARGCTATLGNFAKATFDAISKTYSYLTPDLWKETVFTKSPYQEFTNHLMKTHT
RVSVQRTQAPAVATTG1SWM1eS4Oryctolagus cuniculus0.029596831LEVOMARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNKLKYALTGDEVKKICMQRFIKIDGKVRADI
TYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTI
QIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPR
GKGIRLTIAEERDKRLAAKQSSGG1TK17eS6Oryctolagus cuniculus0.0287519059999999971LEVOMKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKG
HSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRK
PLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRAS
TSKSESSQKG1TM55eS7Oryctolagus cuniculus0.0221689141LEVOMFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLV
RELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDK
AQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQLG1SVB0eS8Oryctolagus cuniculus0.0242633869999999971LEVOMGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIID
VVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISS
LLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGKG1TJW1uS4Oryctolagus cuniculus0.0226415639999999961LEVOMPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRR
LVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFS
LRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEEDB7NZS8uS17Oryctolagus cuniculus0.0184688261LEVOMADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKM
KMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKFG1TRM4uS15Oryctolagus cuniculus0.0172593891LEVOMGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGL
APDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVAG1SP51uS11Oryctolagus cuniculus0.0163027721LEVOMAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQD
VAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRLG1U472eS21Oryctolagus cuniculus0.0090982871LEVOMQSDAGEFVDLYVPRKCSASNRIIGAKDHASIQMNVAEVDKVTGRFNGQFKTYAICGAIRRMGESDDSILRLAKSDGIVS
NNFuS8Oryctolagus cuniculus0.0148655549999999991LEVOMVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFD
VQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFFG1TG89uS12Oryctolagus cuniculus0.0157845490000000021LEVOAGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK
ITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRSG1SZ47eS24Oryctolagus cuniculus0.0155483979999999981LEVOMNDTVTIRTRKFMTNRLLQRKQMVIDVLHPGKATVPKTEIREKLAKMYKTTPDVIFVFGFRTHFGGGKTTGFGMIYDSLD
YAKKNEPKHRLARHGLYEKKKTSRKQRKERKNRMKKVRGTAKANVGAGKKEPRGG1T3D8eS26Oryctolagus cuniculus0.0130155061LEVOMTKKRRNNGRAKKGRGHVQPIRCTNCARCVPKDKAIKKFVIRNIVEAAAVRDISEVSVFDAYVLPKLYVKLHYCVSCAIH
SKVVRNRSREARKDRTPPPRFRPAGAAPPPPPKPMG1TFE8eS27Oryctolagus cuniculus0.0094801861LEVOMPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFR
RKQHG1TZ76eS30Oryctolagus cuniculus0.0144988841LEVOMQLFVRAQELHTLEVTGRETVAQIKAHVASLEGIAPEDQVVLLAGTPLEDEATLGQCGVEALSTLEVAGRMLGGKVHGSL
ARVGKVRGQTLKVAKQEKKKKRTGRAKRRMQYNRRFVNVVPTFGKKKGPNANSG1T8A2uS3Oryctolagus cuniculus0.0311466071LEVOMSARRRRRRAAFRRRAEPFIPISVREPLPFLSAARGGKMAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVT
PTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGV
LRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPSGKIGPKK
PLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTAG1TNM3uS7Oryctolagus cuniculus0.0229134531LEVOMTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHG
RNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGARE
AAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNRG1TFM5eS10Oryctolagus cuniculus0.0171568651LEVOMLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLR
DYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEG1TPV3eS12Oryctolagus cuniculus0.0145389869999999981LEVOMAEEGIAAGGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEPMYVKLVEALCAEHQINLIKVD
DNKKLGEWVGLCKIDREGKPRKVVGCSCVVVKDYGKESQAKDVIEEYFKCKKG1SFR8uS19Oryctolagus cuniculus0.0170491821LEVOMAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLSRGLRRKQHSLLKRLRKAKKEAPPMEKPEVVKTHL
RDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLKG1U0Q2uS9Oryctolagus cuniculus0.0192134651LEVOSARVAPRFPFPVVAPRGEDRHSSAAAMPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEP
VLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGG
PGARARYQKSYRG1SGX4eS17Oryctolagus cuniculus0.0155521191LEVOMGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERER
RDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGAVG1TU13uS13Oryctolagus cuniculus0.0177597769999999971LEVOMSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIP
DWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKKG1TPG3eS19Oryctolagus cuniculus0.0162357961LEVOMPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGAGVGSMTKIYG
GRQRNGVMPSHFSRGSKSVARRVLQALEGLKMVEKDQDWGRKLTPQGQRDLDRIAGQVAAAKKKHG1TN62uS10Oryctolagus cuniculus0.0133987630000000011LEVOMAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRF
QMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADAG1SIZ2eS25Oryctolagus cuniculus0.0137762241LEVOMPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIR
GSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDAG1TDB3eS28Oryctolagus cuniculus0.0078550521LEVOMDTSRVQPIKLARVTKVLGRTGSQGQCTQVRVEFMDDTSRSIIRNVKGPVREGDVLTLLESEREARRLRG1TIB4eS29Oryctolagus cuniculus0.0066908211LEVOMGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLDG1U7M4eS31Oryctolagus cuniculus0.0180040410000000021LEVOMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKR
KKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDKG1SK22RACK1Oryctolagus cuniculus0.0351156521LEVOMTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALS
GSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNS
SNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALC
FSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTRG1SJB4Eukaryotic translation initiation factor 3 subunit AOryctolagus cuniculus0.164902655999999981LEVOMPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQ
VNIKSLEDVVRAYLKLAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQC
LDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSA
ISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMST
RVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNP
LKLCERVTKVLNWVREQPEKEPELQQYVPQLQSNTILRLLQQVAQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDL
QVRIDHTSRTLSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPAHILQEKEEQHQLAVTAYLKN
SRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERL
EQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRIK
DMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHNRLEERKRQRKE
ERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGE
DPLSRRDSRWGDRDSEGTWRKGPEADSEWRRGPPEKEWRRGEGRDDERSHRRDEERPRRVGDDEDRESSLRPDDDRTLRR
GMEDDRGPRRGPDEERFSRRGLEDERPSWRSTDDDRPPRRIGEEDRGSWRHAEDDRPPRRGLDDDRGSWRTADEDRGPRR
MMDEDRGPRRGGADDERPSWRSADDDRGPRRGIDDDRGPRRGLDDDRGPWRNADDDRIPRRGADDDRGPWRNMDDDRMSR
RADDDRISRRGEDSRPGPWRPFVKPGGWREKEKAREESWGPPRESRPPEEREWERERDRDNPDREENDKDPERERDRERD
GDREERFRRPRDEGGWRRGPAEEASSWRDSSRRDDRDRDDRRRERDDRRDLRDRRDLRDDRDRRGPPLRSERDEVSSWRR
ADDRKDDRAEERDPPRRVPPPVLSRDRDRDRDRDRDRDREREREREREGEKEKTSWRAEKDRESLRRTKNETDEDGWTTV
RRG1SMZ5Eukaryotic translation initiation factor 3 subunit COryctolagus cuniculus0.1057061561LEVOMSRFFTTGSDSESESSLSGEELVTKPVGGNYGKQPLLLSEDEEDTKRVVRSAKDKRFEELTNLIRTIRNAMKIRDVTKCL
EEFELLGKAYGKAKSIVDKEGVPRFYIRILADLEDYLNELWEDKEGKKKMNKNNAKALSTLRQKIRKYNRDFESHITNYK
QNPEQSADEDAEKNEEDSEGSSDEDEDEDGVSAAAFLKKKAEAPSGESRKFLKKMEDEDEDSEDSEDDEEWDTGSTSSDS
DSEEEEGKQTALASRFLKKAPTTEEDKKAAEKKREDKAKKKHDRKSKRLDEEEEDNEGGEWERVRGGVPLVKEKPKMFAK
GTEITHAVVIKKLNEILQARGKKGTDRAAQIELLQLLVQIASENNLGEGVIVKIKFNIIASLYDYNPNLATYMKPEMWQK
CLDCINELMDILFANPNIFVGENILEESENLQNVDQPLRVRGCILTLVERMDEEFTKIMQNTDPHSQEYVEHLKDEAQVC
AIIERVQRYLEEKGTTEEICRVYLRRILHTYYKFDYKAHQRQLTPPEGSSKSEQDQAENEGEDSAVLMERLCKYIYAKDR
TDRIRTCAILCHIYHHALHSRWYQARDLMLMSHLQDNIQHADPPVQILYNRTMVQLGICAFRQGLTKDAHNALLDIQSSG
RAKELLGQGLLLRSLQERNQEQEKVERRRQEPFHLHINLELLECVYLVSAMLLEIPYMAAHESDARRRMISKQFHHQLRV
GERQPLLGPPESMREHVVAASKAMKMGDWKTCHSFIINEKMNGKVWDLFPEADKVRTMLVRKIQEESLRTYLFTYSSVYD
SISMETLSDMFELDLPTVHSIISKMIINEELMASLDQPTQTVVMHRTEPTAQQNLALQLAEKLGSLVENNERVFDHKQGT
YGGYFRDQKDGYRKNEGYMRRGGYRQQQSQTAYG1U971Eukaryotic translation initiation factor 3 subunit EOryctolagus cuniculus0.0541998629999999941LEVOMLKIFTCNSHSDLPTNLMAEYDLTTRIAHFLDRHLVFPLLEFLSVKEIYNEKELLQGKLDLLSDTNMVDFAMDVYKNLYS
DDIPHALREKRTTVVAQLKQLQAETEPIVKMFEDPETTRQMQSTRDGRMLFDYLADKHGFRQEYLDTLYRYAKFQYECGN
YSGAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFF
NHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNF
DFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAK
IDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFYG1SUC8Eukaryotic translation initiation factor 3 subunit FOryctolagus cuniculus0.037846731LEVOMATPAVSASAPPATPAATPAAAAAPASAPAAAPAPTPAPAPAAAPAAGAAPAAAADPAAAAAAAGTGQTPASAQAPAQTS
APSLPGPALPGPFPGGRVVRLHPVILASIVDSYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFA
KNMYELHKKVSPNELILGWYATGHDITEHSVLIHEYYSREAPNPIHLTVDTSLQNGRMSIKAYVSTSMGVPGRTMGVMFT
PLTVKYAYYDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQDALSTVLQYAEDVLSGKVSADNTVGRFLMSLVN
QVPKIVPDDFETMLNSNINDLLMVTYLANLTQSQIALNEKLVNLG1SLC2Eukaryotic translation initiation factor 3 subunit HOryctolagus cuniculus0.0410835311LEVOLPTAHAQSVPQMASRKEGTGSGGTSSSSATGVVGKGKGKGGSGDSAVKQVQIDGLVVLKIIKHYQEEGQGTEVVQGVLLG
LVVEDRLEITNCFPFPQHTEDDADFDEVQYQMEMMRSLRHVNIDHLHVGWYQSTYYGSFVTRALLDSQFSYQHAIEESVV
LIYDPIKTAQGSLSLKAYRLTPKLMEVCKEKDFSPEALKKANITFEHMFEEVPIVIKNSHLINVLMWELEKKSAVADKHE
LLSLASSNHLGKNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQRRQQENMQRQSRGEPPLPEEDLSKLFKPPQ
PPARMDSLLIAGQINTYCQNIKEFTAQNLGKLFMAQALQEYNNG1ST95Eukaryotic translation initiation factor 3 subunit KOryctolagus cuniculus0.0251297089999999971LEVOMAMFEQMRANVGKLLKGIDRYNPENLATLERYVETQAKENAYDLEANLAVLKLYQFNPAFFQTTVTAQILLKALTNLPHT
DFTLCKCMIDQAHQEERPIRQILYLGDLLETCHFQAFWQALDENMDLLEGITGFEDSVRKFICHVVGITYQHIDRWLLAE
MLGDLTDSQLKMWMSKYGWSADESGQIFICSQEESIKPKNIVEKIDFDSVSSIMASSQG1T3L2Eukaryotic translation initiation factor 3 subunit LOryctolagus cuniculus0.0710014771LEVOPAGGCAQTSAFPAGPRRAELPAPRRTRGSLSSLSPAVFASAAAMSYPADDYESEAVYDPYAYPSDYDMHTGDPKQDLAYE
RQYEQQTYQVIPEVIRNFIQYFHKTVSDLIDQKVYELQASRVSSDVIDQKVYEIQDIYENSWTKLTERFFKNTPWPEAET
IAPQVGNDAVFLILYKELYYRHIYAKVSGGPSLAQRFESYYNYCNLFNYILNADGPAPLELPNQWLWDIIDEFIYQFQSF
SQYRCKTAKKSEEEIDFLRSNPKIWNVHSVLNVLHSLVDKSNINRQLEVYTSGGDPESVAGEYGRHSLYKMLGYFSLVGL
LRLHSLLGDYYQAIKVLENIELNKKSMYSRVPECQVTTYYYVGFAYLMMRRYQDAIRVFANILLYIQRTKSMFQRTTYKY
EMINKQNEQMHALLAIALTMYPMRIDESIHLQLREKYGDKMLRMQKGDPQVYEELFSYSCPKFLSPVVPNYDSVHPNYHK
EPFLQQLKVFSDEVQQQAQLSTIRSFLKLYTTMPVAKLAGFLDLTEQEFRIQLLVFKHKMKNLVWTSGISALDGEFQSAS
EVDFYIDKDMIHIADTKVARRYGDFFIRQIHKFEELNRTLKKMGQRPG1SED9Eukaryotic translation initiation factor 3 subunit MOryctolagus cuniculus0.0425558321LEVOMSVPAFIDISEEDQAAELRAYLKSKGAEISEENSEGGLHVDLAQIIEACDVCLKEDDKDVESVMNSVVSLLLILEPDKQE
ALIESLCEKLVKFREGERPSLRLQLLSNLFHGMDKNTPVRYTVYCSLIKVAASCGAIQYIPTELDQVRKWISDWNLTTEK
KHTLLRLLYEALVDCKKSDAASKVMVELLGSYTEDNASQARVDAHRCIVRALKDPNAFLFDHLLTLKPVKFLEGELIHDL
LTIFVSAKLASYVKFYQNNKDFIDSLGLLHEQNMAKMRLLTFMGMAVENKEISFDTMQQELQIGADDVEAFVIDAVRTKM
VYCKIDQTQRKVVVSHSTHRTFGKQQWQQLYDTLNAWKQNLNKVKNSLLSLSDTG1SLW8MAGNESIUM ION2.4305e-051MGZINC ION6.5409e-051ZNsingleParticleparticle7.5Quantifoil, UltrAuFoil, R1.2/1.3GOLD300ETHANE100277.15FEI VITROBOT MARK IVGrids were blotted for 2.5s and flash cooled in liquid ethane. FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)COUNTING1-4018.056.91The microscope was equipped with an energy filter with slits aperture of 20eV, installed before the detector.9156471C13.47FSC 0.143 CUT-OFFRELION36100MAXIMUM LIKELIHOODRELIONMAXIMUM LIKELIHOODRELIONemd_21529_msk_1.mapemd_21529_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
400
400400
0
00400400400424.2424.2424.290.090.090.0XYZ-0.0168670830.057210952-0.00005727440.00280703371.06051.06051.0605Unsharpened map before post-processingemd_21529_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
400
400400
0
00400400400424.2424.2424.290.090.090.0XYZ-0.0924470.153741430.000379390140.00537607351.06051.06051.0605Half map 1emd_21529_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)