2020-04-29RELRCSBRCSBRCSB2020-03-082020-04-082020-04-292020-04-29National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM097014United StatesHead masked 3D refined density of the Cricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit used to build the 5-UTR IRES modelNeupane RPisareva VRodriguez CFPisarev AFernandez ISNeupane RPisareva VPRodriguez CFPisarev AVFernandez ISA complex IRES at the 5'-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediate.ElifeUS9202032286223doi:10.7554/eLife.545752050-084XEMD-21529other EM volumeEMD-21530other EM volumeEMD-21531associated EM volumeCricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit from a head masked 3D refinementCricket Paralysis Virus 5-UTR IRES (CrPV 5-UTR-IRES) bound to the small ribosomal subunit from a head masked 3D refinement012345678910111213141516171819202122232425262728293031323334353637383940414243Oryctolagus cuniculussingleParticleparticle7.5ETHANE100277.15FEI VITROBOT MARK IVGrids were blotted for 2.5s and flash cooled in liquid ethane. FEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)COUNTING1-4018.056.91The microscope was equipped with an energy filter with slits aperture of 20eV, installed before the detector.915647GctfC12.79FSC 0.143 CUT-OFFRELION37701MAXIMUM LIKELIHOODRELIONMAXIMUM LIKELIHOODRELIONemd_21531_msk_1.mapemd_21531_additional.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
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