2020-09-02RELRCSBRCSBRCSB2020-06-082020-07-012020-07-012020-09-02National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)P01-AI138398-S1United StatesStructure of the SARS-CoV-2 spike glycoprotein in complex with the C105 neutralizing antibody Fab fragment (state 2)Barnes COBjorkman PJBarnes COWest Jr APHuey-Tubman KEHoffmann MAGSharaf NGHoffman PRKoranda NGristick HBGaebler CMuecksch FLorenzi JCCFinkin SHagglof THurley AMillard KGWeisblum YSchmidt FHatziioannou TBieniasz PDCaskey MRobbiani DFNussenzweig MCBjorkman PJStructures of Human Antibodies Bound to SARS-CoV-2 Spike Reveal Common Epitopes and Recurrent Features of Antibodies.Cell182828202032645326doi:10.1016/j.cell.2020.06.0251097-4172EMD-22128associated EM volumeStructure of the SARS-CoV-2 spike glycoprotein in complex with the C105 neutralizing antibody Fab fragment (state 2)EMD-22127other EM volumeStructure of the SARS-CoV-2 spike glycoprotein in complex with the C105 neutralizing antibody Fab fragment (state 1)6xcnFULLOVERLAPSARS-CoV-2 S 2P trimer complexed with monoclonal Fab fragments from neutralizing antibody C105SARS-CoV-2 S 2P trimer complexed with monoclonal Fab fragments from neutralizing antibody C10501230.75SARS-CoV-2 spike glycoprotein 2P trimer11Severe acute respiratory syndrome coronavirus 2Homo sapiensExpi293FHeLapCAGGSneutralizing antibody C105 Fab123Homo sapiensHomo sapiensExpi293FHeLap3BNCSpike glycoproteinSevere acute respiratory syndrome coronavirus 20.1397889533Homo sapiensLEVOMFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFD
NPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVY
SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQT
LLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRV
QPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSF
VIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPC
NGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFL
PFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNEVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGS
NVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTI
SVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGF
NFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAG
TITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALN
TLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRV
DFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNT
FVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDL
QELGKYEQYIKWPSGRLVPRGSPGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGHHHHHHC105 Fab Heavy ChainHomo sapiens0.0246625063Homo sapiensLEVOQVQLVESGGGLIQPGGSLRLSCAASGFTVSSNYMSWVRQAPGKGLEWVSVIYSGGSTYYADSVKGRFTISRDNSKNTLYL
QMNSLRAEDTAVYYCARGEGWELPYDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWN
SGALTSGVHTFPAVLQSS(UNK)LYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHHHHHHC105 Fab Light ChainHomo sapiens0.0228932273Homo sapiensLEVOQSALTQPPSASGSPGQSVTISCTGTSSDVGGYKYVSWYQQHPGKAPKLMIYEVSKRPSGVPDRFSGSKSGNTASLTVSGL
QAEDEADYYCSSYEGSNNFVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPV
KAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS2-acetamido-2-deoxy-beta-D-glucopyranose0.00022120824NAGsingleParticleparticle1.58100.0NaClsodium chloride20.0Tris-HClUltrAuFoil R1.2/1.3GOLD300GOLDHOLEY ARRAYGLOW DISCHARGEAIR60 s glow discharge in air at 0.20 mA currentETHANE100295FEI VITROBOT MARK IV3 microliters added, blotted for 3 s with 0 blot force. Monodisperse sample taken after size-exclusion chromatographyFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN3002.71.02.5105000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENCollected with 3x3 pattern and beam image shiftGATAN K3 BIOQUANTUM (6k x 4k)159401.8960.01Gain-normalized and collected in counting mode with 40 frames per movieCTFFIND4.1Ab initio volume generated in cryoSPARC1C33.66FSC 0.143 CUT-OFFcryoSPARC2.14.214119MAXIMUM LIKELIHOODcryoSPARC2.14.2MAXIMUM LIKELIHOODcryoSPARC2.14.23cryoSPARC2.14.26VYBBRIGID BODY FITCorrelation coefficientREAL90emd_22128_msk_1.mapemd_22128_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
352
352352
0
00352352352294.272294.272294.27290.090.090.0XYZ-0.126347140.364704640.00398408570.0183946270.8360.8360.836Unsharpened, non-uniform refined volume of C105-SARS-CoV-2 S 2P trimer complex (state 2)