2020-12-02RELRCSBRCSBRCSB2020-06-152020-09-022020-09-022020-12-02Cryo-EM structure of a biotinylated SARS-CoV-2 spike probe in the prefusion state (RBDs down)Cerutti GGorman JKwong PDShapiro LZhou TTeng ITOlia ASCerutti GGorman JNazzari AShi WTsybovsky YWang LWang SZhang BZhang YKatsamba PSPetrova YBanach BBFahad ASLiu LAcevedo SNLMadan Bde Souza MOPan XWang PWolfe JRYin MHo DDPhung EDiPiazza AChang LAbiona OCorbett KSDeKosky BJGraham BSMascola JRMisasi JRuckwardt TSullivan NJShapiro LKwong PDStructure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes.SSRN36396183639618202032742241doi:10.2139/ssrn.36396181556-5068EMD-22161associated EM volumeCryo-EM structure of a biotinylated SARS-CoV-2 spike probe in the prefusion state (RBDs down)6xf5FULLOVERLAPSARS-CoV-2 spike ectodomain biotinylated probe in the prefusion stateSARS-CoV-2 spike ectodomain biotinylated probe in the prefusion state01Severe acute respiratory syndrome coronavirus 2Homo sapiensSpike glycoproteinSevere acute respiratory syndrome coronavirus 20.1387232973Homo sapiensLEVOGPQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVY
FASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYV
SQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTP
GDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPN
ITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIA
PGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFP
LQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIAD
TTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLI
GAEHVNNSYECDIPIGAGICASYQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSM
TKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSK
PSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAG
AALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFG
AISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMS
FPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIG
IVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQGSG
YIPEAPRDGQAYVRKDGEWVLLSTFLGRSGGGLVPQQSGGLNDIFEAQKIEWHEG2-acetamido-2-deoxy-beta-D-glucopyranose0.00022120836NAGsingleParticleparticle7.4ETHANEFEI TITAN KRIOSOTHERBRIGHT FIELDFIELD EMISSION GUN300GATAN K3 BIOQUANTUM (6k x 4k)42.01cryoSPARCC33.45FSC 0.143 CUT-OFFcryoSPARC43176MAXIMUM LIKELIHOODcryoSPARCMAXIMUM LIKELIHOODcryoSPARC6VXXFLEXIBLE FITemd_22161_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
440
440440
0
00440440440470.80002470.80002470.8000290.090.090.0XYZ-0.165453550.7281073-0.000352184170.0182359271.071.071.07Local refinement map (C1 symmetry applied)emd_22161_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
440
440440
0
00440440440470.80002470.80002470.8000290.090.090.0XYZ-0.240258650.77692235-0.00032687620.017612381.071.071.07Unsharpened mapemd_22161_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
440
440440
0
00440440440470.80002470.80002470.8000290.090.090.0XYZ-0.957156061.22481720.000416700960.129733441.071.071.07structure of a biotinylated SARS-CoV-2 spike probe in the prefusion stateemd_22161_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
440
440440
0
00440440440470.80002470.80002470.8000290.090.090.0XYZ-1.17714691.17616770.000418817070.129419061.071.071.07structure of a biotinylated SARS-CoV-2 spike probe in the prefusion state