2021-02-24RELRCSBRCSBRCSB2020-06-152021-02-242021-02-242021-02-24National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI122528United StatesHoward Hughes Medical Institute (HHMI)United StatesCryo-EM map of KSHV ORF68Didychuk ALGates SNMartin AGlaunsinger BDidychuk ALGates SNGardner MRStrong LMMartin AGlaunsinger BAA pentameric protein ring with novel architecture is required for herpesviral packaging.ElifeUS10202133554858doi:10.7554/eLife.622612050-084XEMD-22167associated EM volumePentamer structure of KSHV ORF68Pentamer structure of KSHV ORF6801Human gammaherpesvirus 8Homo sapiens0.536singleParticleparticle1.257.650.0Hepes50.0NaClSodium Chloride50.0KClPotassium Chloride1.0TCEP1.0GlycerolC-flatCOPPER300CARBONHOLEY20.0ETHANE90295FEI VITROBOT MARK IV2 second blot, 3 second wait. FEI TALOS ARCTICAFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN200100.02.71.02.5OTHERNITROGENGATAN K2 SUMMIT (4k x 4k)124088.048.01662435GctfNegative stain initial modelC53.37FSC 0.143 CUT-OFFRELION60772ANGULAR RECONSTITUTIONRELIONANGULAR RECONSTITUTIONRELIONRELION