2020-12-02RELRCSBRCSBRCSB2020-07-112020-07-222020-07-222020-12-02National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI147884United StatesNational Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)AI147884 01A1S1United StatesDistinct conformational states of SARS-CoV-2 spike proteinZhang JCai YFXiao TSPeng HQSterling SMWalsh Jr RMRawson SVolloch SRChen BCai YZhang JXiao TPeng HSterling SMWalsh Jr RMRawson SRits-Volloch SChen BDistinct conformational states of SARS-CoV-2 spike protein.ScienceUS36915861592202032694201doi:10.1126/science.abd42511095-92030038SCIEASEMD-22292associated EM volumeDistinct conformational states of SARS-CoV-2 spike protein6xr8FULLOVERLAPclosed state of pre-fusion SARS-CoV-2 spike glycoproteinclosed state of pre-fusion SARS-CoV-2 spike glycoprotein01closed state of pre-fusion SARS-CoV-2 spike glycoproteinSevere acute respiratory syndrome coronavirus 2Homo sapiens440Spike glycoproteinSevere acute respiratory syndrome coronavirus 20.1449160623Homo sapiensLEVOMFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFD
NPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVY
SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQT
LLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRV
QPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSF
VIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPC
NGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFL
PFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGS
NVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTI
SVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGF
NFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAG
TITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALN
TLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRV
DFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNT
FVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDL
QELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYTLESGGGS
AWSHPQFEKGGGSGGGSGGSSAWSHPQFEK2-acetamido-2-deoxy-beta-D-glucopyranose0.00022120821NAGsingleParticleparticle0.37.50.15NaClsodium chloride0.025Tris-HClTris hydrochloride0.02NP-40Nonidet P40QuantifoilGOLD400CARBONCONTINUOUS0.2GLOW DISCHARGEETHANE100277.15FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K3 BIOQUANTUM (6k x 4k)54.3163799316VXXC32.9FSC 0.143 CUT-OFFRELION71339MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODRELION6VXXA14-1211AB INITIO MODEL