2021-01-13RELRCSBRCSBRCSB2020-09-272020-10-212020-10-212021-01-13National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)P01-AI138938-S1United StatesNational Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)P50-AI150464-13United StatesStructure of the SARS-CoV-2 S 2P trimer in complex with the human neutralizing antibody Fab fragment, C121 (State 2)Abernathy MEBarnes COBjorkman PJBarnes COJette CAAbernathy MEDam KAEsswein SRGristick HBMalyutin AGSharaf NGHuey-Tubman KELee YERobbiani DFNussenzweig MCWest Jr APBjorkman PJSARS-CoV-2 neutralizing antibody structures inform therapeutic strategies.NatureUK588682687202033045718doi:10.1038/s41586-020-2852-11476-46870006NATUASEMD-22735associated EM volumeEMD-22729other EM volumeEMD-22730other EM volumeEMD-22731other EM volumeEMD-22732other EM volumeEMD-22733other EM volumeEMD-22736other EM volumeEMD-22737other EM volumeEMD-22734other EM volume7k8yFULLOVERLAPTernary complex of SARS-CoV-2 S 2P trimer with human neutralizing antibody Fab fragment C121 (state 2)Ternary complex of SARS-CoV-2 S 2P trimer with human neutralizing antibody Fab fragment C121 (state 2)01230.72SARS-CoV-2 S 2P glycoprotein11Severe acute respiratory syndrome coronavirus 2Homo sapiensneutralizing antibody Fab fragment C121123Homo sapiensHomo sapiensSpike glycoproteinSevere acute respiratory syndrome coronavirus 20.1397879693Homo sapiensLEVOMFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFD
NPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVY
SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQT
LLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRV
QPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSF
VIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPC
NGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFL
PFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGS
NVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTI
SVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGF
NFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAG
TITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALN
TLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRV
DFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNT
FVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDL
QELGKYEQYIKWPSGRLVPRGSPGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGHHHHHHC121 Fab Heavy chainHomo sapiens0.0259651621Homo sapiensLEVOQVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGWISPVSGGTNYAQKFQGRVTMTRDTSISTAY
MELSRLRSDDTAVYYCARAPLFPTGVLAGDYYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKD
YFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHHHHH
HC121 Fab Light chainHomo sapiens0.0226199841Homo sapiensLEVOQSALTQPASVSGSPGQSITISCTGTSSDVGSYNLVSWYQQHPGKAPKLMIYEGSKRPSGVSNRFSGSKSGNTASLTISGL
QAEDEADYYCCSYAGSSTLVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVK
AGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS2-acetamido-2-deoxy-beta-D-glucopyranose0.00022120823NAGsingleParticleparticle2.58.00.02Tris0.15NaClsodium chloridGLOW DISCHARGEAIR0.2 mAETHANE100295FEI VITROBOT MARK IV3s blot. Monodisperse sampleFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30050.02.71.02.5105000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGIF Bioquantum20GATAN K3 BIOQUANTUM (6k x 4k)154812.660.01892954cryoSPARC2.15generated ab initio in cryoSPARCC14.4FSC 0.143 CUT-OFFcryoSPARC2.1514999MAXIMUM LIKELIHOODcryoSPARC2.15MAXIMUM LIKELIHOODcryoSPARC2.152cryoSPARC2.156VXX6W41RIGID BODY FITREALemd_22735_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
452
452452
0
00452452452377.872377.872377.87290.090.090.0XYZ-0.069723040.25494920.00043788940.010978140.8360.8360.836un-sharpened main map