2024-03-06RELRCSBRCSBRCSB2020-10-132021-04-142021-04-142024-03-06National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI145069United StatesCryo-electron microscopy structure of the heavy metal efflux pump CusA in a heterogeneous 2 open and 1 closed protomer conformationMoseng MAefflux, pump, heavy metal. copper, silver, closed, open, transporter, MEMBRANE PROTEIN, TRANSPORT PROTEINMoseng MALyu MPipatpolkai TGlaza PEmerson CCStewart PLStansfeld PJYu EWCryo-EM Structures of CusA Reveal a Mechanism of Metal-Ion Export.MbioUS12202133820823doi:10.1128/mBio.00452-212150-75117kf8FULLOVERLAPCusA with heterogeneous protomer conformationsCusA with heterogeneous protomer conformations01Escherichia coliCation efflux system protein CusAEscherichia coli0.115833945000000013Escherichia coliLEVOMGHHHHHHMIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSDVQVIIKTSYPGQAPQIVENQVTYPLTTT
MLSVPGAKTVRGFSQFGDSYVYVIFEDGTDPYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHD
LADLRSLQDWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAGGSSIELAEAEYMVR
ASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIAELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLK
SSLPEGVEIVTTYDRSQLIDRAIDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPALFISLLIITLSFIPIFTLEGQEG
RLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLW
PLNKVGGEFLPQINEGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVETTIQLKP
QEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIKVSGTVLADIDAMAEQIEEVARTVPGVAS
ALAERLEGGRYINVEINREKAARYGMTVADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPM
KQQITLADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAFSGQFELLERANHKL
KLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAI
EAVPSLNNPQTFSEQKLDEALYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIP
AAYKLMWLHRHRVRKP38054COPPER (I) ION6.354599999999999e-052CU1singleParticleparticle7.0Quantifoil R1.2/1.3COPPER300CARBONHOLEYETHANE100277.5FEI VITROBOT MARK IFEI TITAN KRIOSOTHERBRIGHT FIELDFIELD EMISSION GUN300GATAN K2 SUMMIT (4k x 4k)40.013.0FSC 0.143 CUT-OFF43395OTHEROTHERRIGID BODY FITREAL