2021-12-15RELRCSBRCSBRCSB2020-11-022020-12-092020-12-092021-12-15National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)U24 GM129539United StatesOther privateSF349247 (Simons Foundation)United StatesACE2-RBD Focused Refinement Using Symmetry Expansion of Applied C3 for Triple ACE2-bound SARS-CoV-2 Trimer Spike at pH 7.4Gorman JKwong PDShapiro LZhou TTsybovsky YGorman JRapp MCerutti GChuang GYKatsamba PSSampson JMSchon ABimela JBoyington JCNazzari AOlia ASShi WSastry MStephens TStuckey JTeng ITWang PWang SZhang BFriesner RAHo DDMascola JRShapiro LKwong PDCryo-EM Structures of SARS-CoV-2 Spike without and with ACE2 Reveal a pH-Dependent Switch to Mediate Endosomal Positioning of Receptor-Binding Domains.Cell Host MicrobeUS28867879.e5202033271067doi:10.1016/j.chom.2020.11.0041934-6069EMD-22922associated EM volumeACE2-RBD Focused Refinement Using Symmetry Expansion of Applied C3 for Triple ACE2-bound SARS-CoV-2 trimer Spike at pH 7.47kmbFULLOVERLAPACE2-RBD pH 7.4ACE2-RBD pH 7.4012Severe acute respiratory syndrome coronavirus 2Homo sapiensAngiotensin-converting enzyme 2Homo sapiens0.0691536641Homo sapiensLEVOSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQ
ALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRP
LYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGN
VQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHL
KSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKN
MNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYAD3.4.17.23Spike glycoproteinSevere acute respiratory syndrome coronavirus 20.140721265999999981Homo sapiensLEVOVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFAST
EKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPF
LMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSS
SGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNL
CPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQT
GKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSY
GFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDA
VRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEH
VNNSYECDIPIGAGICASYQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTS
VDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKR
SFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQ
IPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISS
VLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQS
APHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNN
TVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQGSGYIPE
APRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGGSGGSAWSHPQFEK2-acetamido-2-deoxy-beta-D-glucopyranose0.0002212085NAGsingleParticleparticle1.07.4PBSC-flat-1.2/1.3COPPERCARBONHOLEYPLASMA CLEANINGETHANE90293FEI VITROBOT MARK IVFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN30070.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENGATAN K3 (6k x 4k)COUNTING131042.053.491541541cryoSPARC3.39FSC 0.143 CUT-OFFcryoSPARC2.15128841NOT APPLICABLEMAXIMUM LIKELIHOODcryoSPARC2.126VXX6M0JFLEXIBLE FITREALemd_22922_msk_1.mapemd_22922_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
420
420420
0
00420420420444.36444.36444.3690.090.090.0XYZ-0.191336772.46736670.000907460340.0243265221.0581.0581.058Unsharpened mapemd_22922_additional_3.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
420
420420
0
00420420420444.36444.36444.3690.090.090.0XYZ-0.254775520.759189960.0019467710.0140377141.0581.0581.058Sharpened C3 unmasked mapemd_22922_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
420
420420
0
00420420420444.36444.36444.3690.090.090.0XYZ0.00.435486850.00191515820.0111741351.0581.0581.058C3 unmasked mapemd_22922_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
420
420420
0
00420420420444.36444.36444.3690.090.090.0XYZ-0.85802274.678680.0015426240.100301961.0581.0581.058Half map Bemd_22922_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
420
420420
0
00420420420444.36444.36444.3690.090.090.0XYZ-1.04932374.4062370.00154018810.100297441.0581.0581.058Half map A