2023-04-05RELRCSBRCSBRCSB2020-12-212021-04-142021-04-142023-04-05Cryo-EM structure of the SARS-CoV-2 spike glycoprotein bound to Fab 2-43Rapp MShapiro LRapp MGuo YReddem ERYu JLiu LWang PCerutti GKatsamba PBimela JSBahna FAMannepalli SMZhang BKwong PDHuang YHo DDShapiro LSheng ZModular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class.Cell RepUS35108950108950202133794145doi:10.1016/j.celrep.2021.1089502211-1247EMD-23165associated EM volumeCryo-EM structure of the SARS-CoV-2 spike glycoprotein bound to Fab 2-437l56FULLOVERLAPCryo-EM structure of the SARS-CoV-2 spike glycoprotein bound to Fab 2-43Cryo-EM structure of the SARS-CoV-2 spike glycoprotein bound to Fab 2-430123Homo sapiensSpike glycoproteinSevere acute respiratory syndrome coronavirus 20.1423993753Homo sapiensLEVOMFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFD
NPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVY
SSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQT
LLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRV
QPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSF
VIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPC
NGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFL
PFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGS
NVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTI
SVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGF
NFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAG
TITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALN
TLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRV
DFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNT
FVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDL
QELGKYEQGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEKGGGSGGGGSGGSA
WSHPQFEKFab 2-43 variable domain heavy chainHomo sapiens0.0142590490000000013Homo sapiensLEVOQVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGWINPNSGGTNYAQKFQGRVTMTRDTSITTAY
MELRRLRSDDTAVYYCARGLGVGCSGGNCYLDYYYMDVWGKGTTVTVSSFab 2-43 variable domain light chainHomo sapiens0.0114845043Homo sapiensLEVOQSALTQPASVSGSPGQSITISCTGTSSDVGGYNYVSWYQQHPGKAPKLMIYDVSKRPSGVSNRFSGSKSGNTASLTISGL
QAEDEGDYYCSSYTSSSTWVFGGGTKLTVL2-acetamido-2-deoxy-beta-D-glucopyranose0.00022120831NAGsingleParticleparticle5.5ETHANEFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300GATAN K3 BIOQUANTUM (6k x 4k)51.6913.6FSC 0.143 CUT-OFF199753MAXIMUM LIKELIHOODMAXIMUM LIKELIHOODemd_23165_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
384
384384
0
00384384384406.27197406.27197406.2719790.090.090.0XYZ-0.390673580.988525570.00060081650.026904831.0581.0581.058Global refinementemd_23165_additional_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
384
384384
0
00384384384406.27197406.27197406.2719790.090.090.0XYZ-0.22154621.55174660.00059699330.0162629691.0581.0581.058Unmasked, unsharpened local refinement of Fab & RBDemd_23165_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
384
384384
0
00384384384406.27197406.27197406.2719790.090.090.0XYZ-1.7617184e-081.89370660.00252807140.0235365851.0581.0581.058Concensus half map A for FSC calculationemd_23165_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
384
384384
0
00384384384406.27197406.27197406.2719790.090.090.0XYZ-2.0004006e-081.72532690.00252964580.023573131.0581.0581.058Concensus half map B for FSC calculation