2021-08-25RELRCSBRCSBRCSB2021-02-172021-07-142021-07-142021-08-25Cryo-EM structure of SARS-CoV-2 spike in complex with neutralizing antibody A23-58.1 that targets the receptor-binding domainZhou TTsybovsky YWang LZhou TZhang YYang ESSchramm CAShi WPegu AOloniniyi OKHenry ARDarko SNarpala SRHatcher CMartinez DRTsybovsky YPhung EAbiona OMAntia ACale EMChang LAChoe MCorbett KSDavis RLDiPiazza ATGordon IJHait SHHermanus TKgagudi PLaboune FLeung KLiu TMason RDNazzari AFNovik LO'Connell SO'Dell SOlia ASSchmidt SDStephens TStringham CDTalana CATeng ITWagner DAWidge ATZhang BRoederer MLedgerwood JERuckwardt TJGaudinski MRMoore PLDoria-Rose NABaric RSGraham BSMcDermott ABDouek DCKwong PDMascola JRSullivan NJMisasi JUltrapotent antibodies against diverse and highly transmissible SARS-CoV-2 variants.ScienceUS373202134210892doi:10.1126/science.abh17661095-92030038SCIEASEMD-23498associated EM volumeEMD-23499other EM volumeFull spike with antibodyEMD-23915other EM volumeStructure from same class member7lrsFULLOVERLAPSARS-CoV-2 spike in complex with neutralizing antibody A789-58.1SARS-CoV-2 spike in complex with neutralizing antibody A789-58.10123The complex was prepared by mixing SARS-CoV-2 spike protein and Fab fragment of antibody A789-58.1 at a molar ratio of 1:3.6.Severe acute respiratory syndrome coronavirus 20.539951Spike glycoproteinSevere acute respiratory syndrome coronavirus 20.0219866521Homo sapiensLEVOITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIA
PGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFP
LQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPantibody A23-58.1 heavy chainHomo sapiens0.0243634259999999971Homo sapiensLEVOQMQLVQSGPEVKKPGTSVKVSCKASGFTFTSSAVQWVRQARGQRLEWIGWIVVGSGNTNYAQKFQERVTITRDMSTSTAY
MELSSLRSEDTAVYYCAAPNCSNVVCYDGFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPV
TVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKantibody A23-58.1 light chainHomo sapiens0.0234750061Homo sapiensLEVOEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYSASSRATGIPDRFSGSGSGTDFTLTISRLE
PEDFAVYFCQQYGTSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNS
QESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECsingleParticleparticle0.57.4100 mM HEPES, pH 7.4, 150 mM NaClETHANE95277FEI VITROBOT MARK IVBlot for 1.5 seconds before plugging.. Complex at 0.5 mg/mL concentration in the presence of 0.005% DDMFEI TITAN KRIOSFLOOD BEAMBRIGHT FIELDFIELD EMISSION GUN300100.02.71.12.1105000.0FEI TITAN KRIOS AUTOGRID HOLDERNITROGENOTHER40.01Local refinement of the SARS-CoV-2 RBD region in complex with the antibody A789-58.1 Fab123016Particle subtraction from the spike-Fab complexcryoSPARC2.157KMS1FOURIER SPACE3.89FSC 0.5 CUT-OFF123016NOT APPLICABLENOT APPLICABLE7KMSOTHERREALemd_23498_msk_1.mapemd_23498_additional_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
512
512512
0
00512512512437.76437.76437.7690.090.090.0XYZ-0.371771631.3092911-0.00063860360.0076960210.8550.8550.855emd_23498_half_map_2.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
512
512512
0
00512512512437.76437.76437.7690.090.090.0XYZ-1.1210621.9502363-0.000189329280.126087960.8550.8550.855emd_23498_half_map_1.map.gz1IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)